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Abstract of Applied Sciences and Engineering

October 2015, Volume 4, 4, pp 22

Effect of Selenium Incorporated In Feed on the Hematological Profile of Oreochromis Niloticus

Muhammad Younus, Sonia Iqbal, Muhammad Sharif Mughal, Arshad Javid, Muhammad Kamran Rafique, Aman Ullah Khan, Noor Khan, Usman Atique

Muhammad Younus 1 

Sonia Iqbal 2 

Muhammad Sharif Mughal 2 Arshad Javid 2 

Muhammad Kamran Rafique 5 

Aman Ullah Khan 1 Noor Khan 5 Usman Atique 2 
  1. College of Veterinary and Animal SciencesJhang, Sub-campus of University of Veterinary and Animal Sciences Lahore. 1

  2. Department of Wildlife and Ecology, University of Veterinary and Animal Sciences Lahore 2

  3. College of Veterinary and Animal SciencesJhang, Sub-campus of University of Veterinary and Animal Sciences Lahore 5


Abstract:

Present study was conducted toevaluate the effect of selenium (Se) supplemented in feed on hematology of Tilapia (Oreochromisniloticus) and variations in different physico-chemical parameters. Three doses of Se i.e. 2, 4, and 8 mg Se/kg of fish feed were prepared and each dose was considered as an independent treatment.Four cemented rectangular tanks (2.896x0.762x0.914 m, length x width x depth) with three equal partitions were used to maintain the fish. Tank 1, 2 and 3 were designated as treatment tanks whereas the 4th one as control. Fifteen fish per tank (5fish/replicate) were stocked having weight between 10 to 25g. The fish were fed at the rate of 3% body weight (BW) twice a day. Variations in different hematological parameters viz.red blood cells (RBC’s), total leucocyte count (TLC) and hemoglobin (Hb) were noted. However, physico-chemical parameters were recorded fortnightly. The results were statistically analyzed by using one way ANOVA. WBC’s were non-significant in treatment-1, 2, 3 and control.  Whereas, neutrophils,RBC’sand Hb was higher in treatment-1(2mg Se/kg). Hb level and neutrophils and RBC’scount was lower in treatment-3(8mg Se/kg) and WBC were lower in treatment-2 (4mg Se/kg).Lymphocytes and monocytes were significantly higher in treatment-3 (8mg Se/kg). However, differences in physico-chemical parameters were not significantly different among the treatments of this study and were within the recommended ranges for Tilapia (Oreochromisniloticus).In conclusion, supplementation of selenium did not altered any hematological parameters however, had significantly improved the physio-biochemical factors and productivity in Tilapia (Oreochromisniloticus).

Keywords:

Selenium, hematology, WBC’s, RBC’s, Physico-chemical parameters, Tilapia

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