Indole Acetic Acid Production by Bacillus Popillae Isolated From Few Legume Plants Rhizosphere Soils

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 Plant rhizosphere soil represents a unique biological niche with diverse micro flora comprised of bacteria, fungi, protozoa and algae. A collection of 185 micro organisms were isolated from the rhizosphere soils of four legume plants (Vigna angularis, Vigna mungo, Vigna radiata and Vigna aconitifolia) and tested for the production of the plant growth hormone indole-3-acetic acid (IAA).The organism with maximum production was characterized and was mutated. The wild and mutated species were tested for the efficiency in IAA production with different medium such as LB medium, King’s B medium and Czapek dox medium. The media were optimized for the production of IAA. The increased quantity of IAA was produced by Bacillus popillae wild (39 µg/ml) and mutant (32 µg /ml) in LB Medium. Production media was prepared with optimized concentrations of carbon source (Bactotryptone 0.40g/25 ml), nitrogen source (Yeast extract 0.18g/25 ml) and mineral source (NaCl 0.30 g/25 ml) for wild and carbon source (Bactotryptone 0.40g g/25 ml), nitrogen source (Yeast extract 0.18g/25 ml) and mineral source (NaCl 0.30 g/25 ml) for mutant strain which produced 65 μg/ml and 40 μg/ml of IAA respectively. The IAA from this strain was extracted, purified and identified by thin layer chromatography and HPLC.