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Animal Review

March 2014, Volume 1, 1, pp 1-10

The Effect of Steaming Process on Fat Soluble Vitamins Content and Fatty Acid Profile in Bluefish and Rainbow Trout Fillets

Stancheva M


Merdzhanova A


Galunska B


Dobreva A.D

Stancheva M 1 ,

Merdzhanova A 1 Galunska B 1 Dobreva A.D 1 
  1. Department of Chemistry, Faculty of Pharmacy, Medical University of Varna, Varna, Bulgaria 1


Fatty acid composition and all-trans-retinol, alpha-tocopherol, and cholecalciferol content was determined and compared in raw and steamed Bluefish and Rainbow trout. Total lipids were extracted by Bligh and Dyer method followed by GC-MS. All-trans-retinol, cholecalciferol and alpha-tocopherol were analyzed simultaneously using HPLC. In comparison with raw fish fillets, analyzed fat soluble vitamin’s content in steamed fish filletsfor the Trout and Bluefish decreased significantly to about 54.2% and 49.8% for retinol and 32.6% and 43.5% for alpha-tocopherol, respectively. After steaming, the cholecalciferol amounts in processed fillets decreased significantly only in Rainbow trout (23.5%), whereas in Bluefish the losses were non-significant. After cooking, the polyunsaturated fatty acid content changed significantly in the Rainbow trout (45.8%), whereas the variations in the Bluefish were minor. The major PUFA in all samples were linoleic acid (LA) and docosahexaenoic acid (DHA). PUFA/SFA ratios were between 1.01 and 1.68 for both species. Steaming increases PUFA/SFA ratio by 8.33% in Rainbow trout, but does not affect this ratio in Bluefish.
Contribution/ Originality


Steaming Process, Fatty Acid Profile, Rainbow Trout Fillets


  1. Μ. Karapetkova and Μ. Jivkov, Fishes in Bulgaria. Bulgaria: Geya-Libris, Sofia, 2006.
  2. E. O. Elvevoll and D. G. James, "Scientific and ethical challenges in agriculture to meet human needs. FAO's Fishery Industries Division, FAO/WHO." Available, 1994.
  3. R. Krauss, R. J. Deckelbaum, E. Fisher, B. V. Howard, and R. H. Knopp, "Dietary guidelines for health American adults-A statement for health professionals from the nutrition committee," Am. Heart Ass., vol. 94, pp. 1795-1800, 1996.
  4. C. Cahu, P. Salen, and M. D. Lorgeril, "Farmed and wild fish in the prevention of cardiovascular diseases: Assessing possible differences in lipid nutritional values," Nutr. Metabolism and Cardiovascular Diseases, vol. 14, pp. 34–41, 2004.
  5. Report of the Joint FAO/WHO Expert Consultation on the Risks and Benefits of Fish Consumption.
  6. D. Mozaffarian and E. Rimm, "Fish intake, contaminants, and human health evaluating the risks and the benefits," J. Am. Med. Ass., vol. 296, pp. 1885-1899, 2006.
  7. National Center of Public Health and Analyses (NCPHA), Available, 2011.
  8. AOAC, "Moisture in meat. Official method of analysis 950.46 of AOAC International," 1991.
  9. D. A. Dobreva, B. Galunska, and M. Stancheva, "Liquid chromatography method for the simultaneous quantification of fat soluble vitamins in fish tissue," Scr. Sci. Med., Varna Medical University, vol. 43, pp. 276-279, 2011.
  10. E. Bligh and W. J. Dyer, "A rapid method of total lipid extraction and purification," Can. J. Biochem. Phys., vol. 37, pp. 913–917, 1959.
  11. EN ISO 5509, Animal and vegetable fats and oils - preparation of methyl esters of fatty acids. Geneva: International Organization for Standartization, 2001.
  12. EN ISO 5508, Animal and vegetable fats and oils - analysis by gas chromatography of methyl esters of fatty acids. Geneva: International Organization for Standartization, 2000.
  13. Z. E. Sikorski and E. K. Olakowska, Chemical and functional properties of food lipids. Boca Raton: CRC Press, 2002.
  14. D. Larsen, S. Y. Quek, and L. Eyres, "Effect of cooking method on the fatty acid profile of New Zealand King salmon (Oncorhychustshawytscha)," Food Chem., vol. 119, pp. 785-790, 2010.
  15. P. Mattila, V. Piironen, E. Uusi-Rauva, and P. Koivistoinen, "Cholecalciferol and 25-Hydroxycholecalciferol contents in fish and fish products," J. Food Comp. Anal., vol. 8, pp. 232-243, 1995.
  16. P. Mattila, R. Ronkainen, K. Lehikoinenand, and V. Piironen, "Effect of household cooking on the vitamin D content in fish, eggs and wild mushrooms," J. Food Comp. Anal., vol. 12, pp. 153-160, 1999.
  17. B. Ersoy and A. Ozeren, "The effect of cooking methods on mineral and vitamin contents of African catfish," Food Chem., vol. 115, pp. 419-422, 2009.
  18. N. Erkan, A. Selcuk, and O. Ozden, "Amino acid and vitamin composition of raw and cooked horse mackerel," Food Anal. Meth., vol. 3, pp. 269-275, 2010.
  19. M. Gladyshev, N. Sushnichk, G. Gubabenko, S. Demircheva, and G. Kalachova, "Effect of boiling and frying on the content of essential polyunsaturated fatty acid in muscle tissue of four fish species," Food Sci., vol. 101, pp. 1694-1700, 2007.
  20. A. Kolakowska, Z. Domiszewski, and G. Bienkiewicz, "Effects of biological and technological factors on the utility of fish as a source of n3 PUFA, Omega 3 fatty acid research," Nova Sc. Publ., pp. 83-107, 2006.
  21. E. W. Murphy, P. E. Criner, and B. C. Gray, "Comparison of methods for determining retentions of nutrients in cooked foods," J.Agr.Food Chem., vol. 23, pp. 1153-1157, 1975.
  22. L. Kalyoncu, Yaman.Y.A., and D. Aktumsek, "Determination of the seasonal changes on total fatty acid composition of Rainbow trout, Oncorhynchusmykiss in Ivriz Dam Lake, Turkey," Afr. J. Biot., vol. 9, pp. 4783-4787, 2010.
  23. S. Saglik and S. Imre, "?3–Fatty acids in some fish species from Turkey," J. Food Sci., vol. 66, pp. 210-212, 2001.
  24. Y. Özogul, F. Özogul, and S. Alagoz, "Fatty acid profile and fat content of commercially important seawater and freshwater fish species of Turkey: A comparative study," Food Chem., vol. 103, pp. 217-223, 2007.
  25. X. Su and J. R. Babb, "The effect of cooking process on the total lipid and n-3 LC-PUFA content of Australian bass strait scallops, pectenfumatus," As. Pac. J. Cl.Nutr., vol. 16, pp. 407-411, 2007.
  26. W. Steffens and M. Wirth, "Freshwater fish - an important source of n-3 polyunsaturated fatty acids: A review," Arch. Pol. Fish, vol. 13, pp. 5-16, 2005.
  27. A. Simopoulos and L. Cleland, "Importance of the ratio of Omega-6/Omega-3 essential fatty acids: Evolutionary aspects. Omega-6/Omega-3 essential fatty acid ratio: The scientific evidence," World Rev. Nutr. Diet., vol. 92, pp. 1-22, 2003.
  28. R. J. Henderson and D. R. Tocher, "The lipid composition and biochemistry of freshwater fish," Prog. Lip. Res., vol. 26, pp. 281-347, 1987.
  29. Department of Health, Nutritional aspects of cardiovascular disease: Health and social subjects. London, UK: HMSO, 1994.


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