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Antifungal Activities of Selected Plant Extracts in In-Vitro Control of Anthracnose and Root Rot Diseases on Cucumber (Cucumis sativus B.)

Wuraola Funmi Ogundipe

,

Ayotunde Ajoke Pitan

,

Oluwafemi Michael Adedire

,

Adekunle Opeyemi Farinu

,

Barry Oyeyemi Oyewole

Wuraola Funmi Ogundipe 1

Ayotunde Ajoke Pitan 2 

Oluwafemi Michael Adedire 3 Adekunle Opeyemi Farinu 3 Barry Oyeyemi Oyewole 2 

  1. Federal College of Agriculture, Moor Plantation Ibadan, Nigeria. 1

  2. National Horticultural Research Institute, Ibadan, Nigeria. 2

  3. Federal College of Agriculture, Moor Plantation, Ibadan, Nigeria. 3

Pages: 80-86

DOI: 10.18488/journal.70.2021.82.80.86

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Article History:

Received: 08 January, 2021
Revised: 05 February, 2021
Accepted: 26 February, 2021
Published: 11 March, 2021


Abstract:

An in-vitro experiment was conducted at National Centre for Genetic Resources and Biotechnology and the Nigeria Agricultural Quarantine Service, Moor plantation, Ibadan. The experiment was carried out to test the antifungal efficacy of some plant extracts (Ageratum conyzoides, Azadirachta indica, Morinda lucida, and Chromolaena odorata) and a chemical fungicide (mancozeb). The mycelial growth inhibition potentials of five concentrations of aqueous plant extracts were assayed at different incubation periods on the growth of Colletotrichum orbiculare and Lasiodiplodia theobromae. The experiment was carried out in a Completely Randomized Design (CRD) with five replications. A 3 mm mycelial disc of each test fungus was placed at the center of a 9 cm Petri dish containing 5, 10, 15, 20, and 25 g of the plant extracts or 0.25g/100mL of mancozeb (synthetic fungicide) in Potato Dextrose Agar. The results obtained revealed that all the plant extracts, at all concentrations, significantly inhibited the growth of these mycopathogens, with 25g C. odorata having the highest percentage inhibition of 70.78% and 73.68% at 48 and 96 hours of incubation on C. orbiculare and L. theobromae respectively. All the selected aqueous extracts inhibited more than 50% C. orbiculare mycelial growth. Antifungal extracts recovered from the selected plants could be further purified to improve and characterize their fungicidal activities in controlling plant diseases. Extracts of plant materials, which are readily available to the farmers, are better alternatives to the commonly used hazardous, synthetic fungicides.
Contribution/ Originality
This paper’s primary contribution is finding that all the plant extracts used against cucumber pathogens had significant inhibitory properties inhibited at different concentrations and incubation periods respectively.

Keywords:

Plant extracts, Inhibition, Lasiodiplodia theobromae, Colletotrichum orbiculare, Mycelial growth, In-vitro.

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Funding:

This study received no specific financial support.

Competing Interests:

The authors declare that they have no competing interests.

Acknowledgement:

All authors contributed equally to the conception and design of the study.

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