Contact Us

For Marketing, Sales and Subscriptions Inquiries
Rockefeller Center, 45 Rockefeller Plaza
20th Flr Unit #5, New York, NY 10111
United States

Conference List

Antifungal Activities of Selected Plant Extracts in In-Vitro Control of Anthracnose and Root Rot Diseases on Cucumber (Cucumis sativus B.)

Wuraola Funmi Ogundipe


Ayotunde Ajoke Pitan


Oluwafemi Michael Adedire


Adekunle Opeyemi Farinu


Barry Oyeyemi Oyewole

Wuraola Funmi Ogundipe 1

Ayotunde Ajoke Pitan 2 

Oluwafemi Michael Adedire 3 Adekunle Opeyemi Farinu 3 Barry Oyeyemi Oyewole 2 

  1. Federal College of Agriculture, Moor Plantation Ibadan, Nigeria. 1

  2. National Horticultural Research Institute, Ibadan, Nigeria. 2

  3. Federal College of Agriculture, Moor Plantation, Ibadan, Nigeria. 3

Pages: 80-86

DOI: 10.18488/journal.70.2021.82.80.86

Share :

Article History:

Received: 08 January, 2021
Revised: 05 February, 2021
Accepted: 26 February, 2021
Published: 11 March, 2021


An in-vitro experiment was conducted at National Centre for Genetic Resources and Biotechnology and the Nigeria Agricultural Quarantine Service, Moor plantation, Ibadan. The experiment was carried out to test the antifungal efficacy of some plant extracts (Ageratum conyzoides, Azadirachta indica, Morinda lucida, and Chromolaena odorata) and a chemical fungicide (mancozeb). The mycelial growth inhibition potentials of five concentrations of aqueous plant extracts were assayed at different incubation periods on the growth of Colletotrichum orbiculare and Lasiodiplodia theobromae. The experiment was carried out in a Completely Randomized Design (CRD) with five replications. A 3 mm mycelial disc of each test fungus was placed at the center of a 9 cm Petri dish containing 5, 10, 15, 20, and 25 g of the plant extracts or 0.25g/100mL of mancozeb (synthetic fungicide) in Potato Dextrose Agar. The results obtained revealed that all the plant extracts, at all concentrations, significantly inhibited the growth of these mycopathogens, with 25g C. odorata having the highest percentage inhibition of 70.78% and 73.68% at 48 and 96 hours of incubation on C. orbiculare and L. theobromae respectively. All the selected aqueous extracts inhibited more than 50% C. orbiculare mycelial growth. Antifungal extracts recovered from the selected plants could be further purified to improve and characterize their fungicidal activities in controlling plant diseases. Extracts of plant materials, which are readily available to the farmers, are better alternatives to the commonly used hazardous, synthetic fungicides.
Contribution/ Originality
This paper’s primary contribution is finding that all the plant extracts used against cucumber pathogens had significant inhibitory properties inhibited at different concentrations and incubation periods respectively.


Plant extracts, Inhibition, Lasiodiplodia theobromae, Colletotrichum orbiculare, Mycelial growth, In-vitro.


Abbey, B., Nwachoko, N., & Ikiroma, G. (2017). Nutritional value of cucumber cultivated in three selected states of Nigeria. Biochemistry and Analytical Biochemistry, 6(3), 1-3. Available at:

Abd-El-Khair, H., & Haggag, W. M. (2007). Application of some Egyptian medicinal plant extracts against potato late and early blights. Research Journal of Agriculture and Biological Sciences, 3(3), 166-175.

Abeer, A. E., Hoballah, E. M., Abdel-Halim, K. Y., & Sanaa, A. M. (2017). Antifungal activities of some botanical extracts and synthetic compounds against down mildew in cucumber plants. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 8(3), 798-805.

Akinbode, O. (2010). Evaluation of antifungal efficacy of some plant extracts on Curvularia lunata, the causal organism of maize leaf spot. African Journal of Environmental Science and Technology, 4(11), 797-800.

Amadioha, A. C., & Obi, V. I. (1999). Control of anthracnose disease of cowpea Cymbopogon citratus and Ocimum gratissimum. Acta of Phytopathology and Entomology Hungerica, 34(4), 85-89.

Biu, A., Yusufu, S., & Rabo, J. (2009). Phytochemical screening of Azadirachta indica (Neem)(Meliaceae) in Maiduguri, Nigeria. Bioscience Research Communications, 21(6), 281-283.

Bobbarala, V. V., Katikala, P. K., Naidu, K. C., & Penumajji, S. (2009). Antifungal activity of selected plant extracts against phytopathogenic fungi Aspergillus niger F2723. Indian Journal of Science and Technology, 2(4), 6839-6846.

Burgess, L. W., Knight, T. E., Tesoriero, L., & Phan, H. T. (2008). Diagnostic manual for plant diseases in Vietnam. ACIAR Monograph No. 129, ACIAR: Canberra, pp 210.

Dania, V., Fadina, O., Ayodele, M., & Kumar, P. L. (2015). Allelopathic potential of some biocontrol agents for the control of fungal rot of yellow yam (Dioscorea cayenensis Lam). African Journal of Biotechnology, 14(6), 474-481. Available at:

De Billerbeck, V. G., Roques, C. G., Bessière, J.-M., Fonvieille, J.-L., & Dargent, R. (2001). Effects of Cymbopogon nardus (L.) W. Watson essential oil on the growth and morphogenesis of Aspergillus niger. Canadian journal of microbiology, 47(1), 9-17. Available at:

Gurjar, M. S., Ali, S., Akhtar, M., & Singh, K. S. (2012). Efficacy of plant extracts in plant disease management. Agricultural Sciences, 3(3), 425-433.

Gwa, V., & Nwankiti, A. (2017). Efficacy of some plant extracts in in-vitro control of Colletotrichum species, causal agent of yam (Dioscorea rotundata Poir.) tuber rot. Asian Journal of Plant Science and Research, 7(2), 8-16.

Iwuagwu, C., Onejeme, F., Ononuju, C., Umechuruba, C., & Nwogbaga, A. (2018). Effects of plant extracts and synthetic fungicides on the radial growth of Phoma oryzae on Rice (Oryza sativa L.) in some rice growing areas of South Eastern Nigeria. J Plant Pathol Microbiol, 9(12), 1-5.

Mares, D., Tosi, B., Poli, F., Andreotti, E., & Romagnoli, C. (2004). Antifungal activity of Tagetes patula extracts on some phytopathogenic fungi: Ultrastructural evidence on Pythium ultimum. Microbiological Research, 159(3), 295-304. Available at:

Nene, Z. H., & Thapilyal, M. (2002). Management of mushroom pathogens through botanicals. Indian Phytopathology, 58, 189-193.

Ngane, A. N., Etame, R. E., Ndifor, F., Biyiti, L., Zollo, P. A., & Bouchet, P. (2006). Antifungal activity of Chromolaena odorata (L.) King & Robinson (Asteraceae) of Cameroon. Chemotherapy, 52(2), 103-106.

Onifade, A. (2000). Antifungal effect of Azadirachta indica A JUSS extracts on Colletotrichum lindemuthianum. Global Journal of Pure and Applied Sciences, 6(3), 425-428.

Perez-Sanchez, R., Infante, F., Galvez, C., & Ubera, J. (2007). Fungitoxic activity against phytopathogenic fungi and the chemical composition of Thymus zygis essential oils. Food Science and Technology International, 13(5), 341-347. Available at:

Sidra, J., & Bashir, U. (2012). Antifungal activity of different extracts of Ageratum conyzoides for the management of Fusarium solani. African Journal of Biotechnology, 11(49), 11022-11029.

Tohamy, M., Aly, A., Abd-El-Moity, T., Atia, M., & Abed-Rl-Moneim, M. (2002). Evaluation of some plant extracts in control damping-off and mildew diseases of cucumber. Egyptian Journal of Phytopathology, 30(2), 71-80.

Utobo, E. B., Ekwu, L. G., Nwogbaga, A. C., & Nwanchor, K. (2016). The Efficacy of eco-friendly botanicals in the management of damping-off and downy mildew. International Journal of Science and Research, 5(7), 1972 – 1977.


Google Scholor ideas Microsoft Academic Search bing Google Scholor


This study received no specific financial support.

Competing Interests:

The authors declare that they have no competing interests.


All authors contributed equally to the conception and design of the study.

Related Article

( 1 ) Antifungal Activities of Selected Plant Extracts in In-Vitro Control of Anthracnose and Root Rot Diseases on Cucumber (Cucumis sativus B.)
( 2 ) Gender Differentials in Labour Market Participation of Rural Households in Non-Farm Activities in Oyo State, Nigeria
( 3 ) Insect Activities and their Impact on the Yield of Abelmoschus esculentus L ( Malvaceae) in Bambili ( Mezam - Cameroon)
( 4 ) Relationship between Human Activities and Deforestation in Karongi District of Rwanda
( 6 ) Nutritional Properties of Some Novel Selected Fish Species in Khuzestan Province, Iran
( 7 ) Blood Picture and Selected Oxidative Stress Biomarkers in Dromedary Camels Naturally Infected With Trypanosoma Evansi
( 8 ) Determination of Milk Composition, Bacteriology and Selected Blood Parameters of Dairy Goats Under Different Feeding Systems
( 9 ) Evaluation of Selected Botanical Extracts against Mendi Termite Macrotermes subhyalinus (Isoptera: Termitidae), under Laboratory Condition
( 10 ) Assessment of Potassium Leaching Behaviour in Selected Soils of Southeastern Nigeria
( 11 ) The Value of Selected Ecosystem Services: A Case Study of East Mau Forest Ecosystem, Kenya
( 12 ) Contextual Estimation of Marketable and Marketed Surplus of Selected Seasonal Fruits: A Study Based on Chittagong Hill Tracts (CHT) of Bangladesh
( 13 ) Adoption Impact of Improved Cowpea Variety in Selected Areas of Chattogram District of Bangladesh
( 14 ) An Assessment of Food Hygiene Practices among Food Vendors in Some Selected Basic Schools in the Birim Central Municipality
( 15 ) Death in the Mouth: The Current Status of Pesticide Residues in Cured Fish from Selected Markets in Rivers State Nigeria
( 16 ) Carbon Sequestration Assessment of Selected Campus Champion Trees
( 17 ) Production and Value Chain Analysis of Lentil in Some Selected Areas of Bangladesh
( 18 ) Ovitrap Surveillance of the Dengue Vectors Aedes aegypti and Aedes albopictus in Selected Endemic Areas in Sandakan, Sabah
( 19 ) Socio Economic Determinant and Profitability of Bari Mungbean Variety in Some Selected Areas of Bangladesh
( 21 ) Management of Fusarium Wilt Disease of Tomato (Solanum lycopersicum L.) Using Selected Plant Extracts
( 22 ) Efficacy of Allicin (Allium Sativum Linn.) Against Bipolaris Sorokiniana in Barley Plants
( 23 ) Ethnomedicinal Survey of Plants Used In Treating Sexually Transmitted Diseases in Abia State, Nigeria
( 24 ) Review: Plant Resistance to Agriculture Insect Pests
( 25 ) Evaluation of In Vitro Protocols for Elimination of Banana Streak Virus from Tissue Cultured Explants in Banana Seedling Production
( 26 ) Effect of Ethanol and Petroleum Ether Extracts of Some Medicinal Plants on Sclerotiumrolfsii, Causal Agent of Postharvest Rot of Yam (Dioscorea Spp.)
( 27 ) The Particle Size and Thermal Properties of Flour from three Plantain (Musa Paradisiaca) Cultivars Grown in Nigeria
( 28 ) Management of Root-Knot Nematode, Meloidogyne Javanica Infecting Sunflower by Some Composted Plant or Animal Residues as Soil Amendments in Egypt
( 29 ) Effect of a Plant Extract in Several Traits of Plymouth Rock Barred Hens and Pullets Challenged with Salmonella Typhimurium in a Rural Village in Central Mexico
( 30 ) Duration of Freezing Influences Sensory Attributes of Cassava (Manihot esculenta Crantz) and Plantain (Musa paradisiaca AAB)
( 31 ) Patterns of Plant Community Formation and Vegetation Structure in the Afro- Alpine Vegetation of Simien Mountains National Park, Ethiopia
( 32 ) Evaluation of Repellency of Plant-Derived Insecticide against Brinjal and Pepper Pest Populations
( 33 ) Effect of Planting Date on Growth and Yield of Bambara Groundnut (Vigna subtermnea (L) Verdc.) Varieties in Rainforest Zone of Delta State
( 34 ) Chemical Changes Associated with Repetitive Re-Use of Vegetable Oil during Deep Frying of Bean Cake, Plantain and Yam
( 35 ) Comparing the Molluscicidal Action of Metaldehyde, Oxamyl, Potassium Nitrate and a Botanical Insecticide for Managing Limacolaria Spp Infesting Banana Plantations in Cameroon
( 36 ) Study of Heavy Metals Variability and their Effect on Plant Growth in Kanzenze River of Upper Akagera Catchment, Rwanda
( 37 ) Plant Diversity and Regeneration Potentials in Protected Area Forests of Sierra Leone
( 39 ) Heat Tolerance Stability of Bread Wheat Genotypes under Early and Late Planting Environments through Stress Selection Indices
( 40 ) Penicillium and Aspergillus Species Characterization: Adaptation to Environmental Factors and Sensitivity to Aqueous Medicinal Plants Extracts
( 42 ) Olfactory Response of an Assassin Bug, Rhynocoris Longifrons (Insecta: Hemiptera: Reduviidae) To the Hexane Extracts of Different Agricultural Insect Pests
( 44 ) Evaluation of the Phytochemical, Antioxidant and Antimicrobialproperties of Extracts from Chrysophyllum Albidum (African Star Apple) Leaf
( 50 ) Growing Jatropha Curcas and Jatropha Gossypiifolia as a Interculture with Sunflower for Control of Meloidogyne Javanica in Egypt
( 51 ) Experimental Model Validation and Control of a Lactic Fermentation Process
( 52 ) Insecticide Spraying Regime to Control Insect Pest of Cowpea: Management and Monitored Application of Cypermethrin in Lomami Province, DR Congo
( 53 ) High-Sensitivity Testing of Effectiveness of Citrus Limon, Vitis Vinifera and Citrus Sinensis in the Postharvest Control of Callosobruchus Maculatus Fabricius (Coleoptera: Chrysomelidae) Infestation of Cowpea Seeds
( 54 ) Two Stubborn Storage Insect Pests, Callosobruchus maculatus and Sitophilus zeamais: Biology, Food Security Problems and Control Strategies
( 55 ) On-Farm Evaluation of Conservation Agriculture Practice on Weed Control and Yield of Wheat in Northern Bangladesh
( 56 ) Chemical Control of Alternaria Brown Spot on Mandarins Cultivars in Tunisia
( 59 ) Effect of Different Concentrations of Vermicompost (Biohumus) On the Root Collar Diameter and Height Growth in the Seedlings of Anatolian Black Pine
( 61 ) Effects of Different Storage Conditions on Rooting and Shooting Performance of Grapevine (Vitis Vinifera L.) Cuttings in Hydroponic Culture System
( 62 ) Changes in Certain Agronomic Characteristics of Table Grape Cultivar Alphonse Lavallee’ in Response to Water Deficit under the Different Rootstock Effect
( 63 ) Root Density, Distribution and Yield Relationships of High Yielding Sugarcane Varieties Under Sandy Soil Condition
( 65 ) Farmer’s Decision To Practice Crop Rotation in Arsi Negelle, Ethiopia: What are the Determinants?
( 66 ) Effect of Some Botanicals against Termites, Macroterms Spp. (Isoptera: Termitidae) Under Laboratory Conditions
( 67 ) Soil-Blade Interaction of a Rotary Tiller: Soil Bin Evaluation
( 68 ) Protective Bioactivity of Moringa (Moringa Oleifera Lam.) Seed Oil against Callosobrochus Maculatus (F.) Infestation in Stored Cowpea (Vigna Unguiculata L. Walp.)
( 69 ) Urban Area - Review of Protective Forest Management