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Current Research in Agricultural Sciences

June 2021, Volume 8, 1, pp 31-36

Azotobacter and Total Bacterial Population in Enriched Compost: Preliminary Study

Reginawanti Hindersah

,

Agustinus Marthin Kalay

Reginawanti Hindersah 1

Agustinus Marthin Kalay 2

  1. Faculty of Agriculture, Universitas Padjadjaran Jalan Raya Bandung-Sumedang, Indonesia. 1

  2. Faculty of Agriculture, Universitas Pattimura, Jalan Ir. Putuhena, Poka Campus, Indonesia. 2

Pages: 31-36

DOI: 10.18488/journal.68.2021.81.31.36

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Article History:

Received: 14 January, 2021
Revised: 11 February, 2021
Accepted: 05 March, 2021
Published: 25 March, 2021


Abstract:

Compost enrichment with nitrogen fixing Azotobacter is a method to enhance the used of biofertilizer in sustainable agriculture. The objective of a laboratory experiment was to verify the change in Azotobacter and total bacteria population in two kind of compost after inoculation with nitrogen fixing A. chroococcum. The experiment was setup in completely randomized design with four combination treatments of sterilized and unsterilized composted manure as well as vermicompost. The control treatments are the sterilized one. The results verified that irrespective of treatments, Azotobacter enrichment increased Azotobacter but reduced total bacteria population after 18-day incubation compared to the composts before treatment. At the end of experiment, the population of Azotobacter in both sterilized and unsterilized vermicompost were slightly lower than in manure; but the count total heterotroph bacteria in both organic matter was similar. The acidity of both enriched compost was neutral while their humidity was declined to 18-19% at the end of experiment. This experiment showed that inoculating the compost with Azotobater maintain the Azotobacter count but decline the total bacteria. This suggested that negative interaction between Azotobacter and other heterotroph bacteria in compost might be occured.
Contribution/ Originality
This study is one of very few studies about the viability of total bacteria and Azotobacter in Azotobacter-enriched compost. Compost enrichment with Azotobacter enhanced Azotobacter population but decreased total bacteria decreased in the compost. This enriched compost can serve as organic matter and Azotobacter inoculant once introducing to the soil.

Keywords:

Azotobacter, Total bacteria, Sterilized compost, Compost quality, Microbial density, Compost acidity, Compost humidity.

Reference:

[1]          M. M. Tahat, K. M. Alananbeh, Y. A. Othman, and D. I. Leskovar, "Soil health and sustainable agriculture," Sustainability, vol. 12, pp. 1-26, 2020.

[2]          C. Gougoulias, J. M. Clark, and L. J. Shaw, "The role of soil microbes in the global carbon cycle: Tracking the below-ground microbial processing of plant-derived carbon for manipulating carbon dynamics in agricultural systems," Journal of the Science of Food and Agriculture, vol. 94, pp. 2362-2371, 2014. Available at: https://doi.org/10.1002/jsfa.6577.

[3]          C. Francou, M. Poitrenaud, and S. Houot, "Stabilization of organic matter during composting: Influence of process and feedstocks," Compost Science & Utilization, vol. 13, pp. 72-83, 2013. Available at: https://doi.org/10.1080/1065657x.2005.10702220.

[4]          E. J. Rubio, M. S. Montecchia, M. Tosi, F. D. Cassán, A. Perticari, and O. S. Correa, "Genotypic characterization of azotobacteria isolated from argentinean soils and plant-growth-promoting traits of selected strains with prospects for biofertilizer production," The Scientific World Journal, vol. 51, pp. 1-12, 2013. Available at: https://doi.org/10.1155/2013/519603.

[5]          V. Patil, "Production of indole acetic acid by Azotobacter sp," Recent Research in Science and Technology, vol. 3, pp. 14-16, 2011.

[6]          R. Hindersah, "Growth and exopolysachharide composition of nitrogen fixing bacteria Azotobacter spp. in the presence of cadmium," presented at the Proc Seminar Nasional Masyarakat Biodiversitas Indonesia, 2015.

[7]          E. G. d. Moura, C. Gehring, H. Braun, A. D. S. L. Ferraz Junior, F. d. O. Reis, and A. D. C. F. Aguiar, "Improving farming practices for sustainable soil use in the humid tropics and rainforest ecosystem health," Sustainability, vol. 8, pp. 1-21, 2016. Available at: https://doi.org/10.3390/su8090841.

[8]          S. Gaind, A. K. Pandey, and Lata, "Microbial biomass, P-nutrition, and enzymatic activities of wheat soil in response to phosphorus enriched organic and inorganic manures," Journal of Environmental Science and Health Part B, vol. 41, pp. 177-187, 2006. Available at: https://doi.org/10.1080/03601230500365044.

[9]          B. Nilay, N. Deka, J. Deka, I. Barua, D. Nath, and B. Medhi, "Enrichment of compost through microbial inoculation–effect on quality," International Journal of Current Research, vol. 6, pp. 8026-8031, 2014.

[10]        R. Kavitha and P. Subramanian, "Bioactive compost-a value added compost with microbial inoculants and organic additives," Journal of Applied Sciences, vol. 7, pp. 2514-2518, 2007. Available at: https://doi.org/10.3923/jas.2007.2514.2518.

[11]        K. Rajasekar, T. Daniel, and N. Karmegam, "Microbial enrichment of vermicompost," International Scholarly Research Notices, vol. 2012, p. 13, 2012.

[12]        B. M. Sudjana and V. Subardja, "Enrichment of mushroom compost heap quality using azotobacter and pseudomonas as a renewable ameliorant," International Journal of Agriculture Innovations and Research, vol. 5, pp. 866-871, 2017.

[13]        E. Sanders, "Aseptic laboratory techniques: Plating methods," Journal of Visualized Experiments, vol. 63, p. e3064, 2012. Available at: https://doi.org/10.3791/3064.

[14]        AOAC, Official methods of analysis, 19th ed. Washington DC, USA: Association of Official Analytical Chemist, 2012.

[15]        E. A. Paul and F. E. Clark, Soil microbiology and biochemistry, 2nd ed. San Diego: Academic Press, 1996.

[16]        H. Mukhtar, H. Bashir, and A. Nawaz, "Optimization of growth conditions for Azotobacter species and their use as biofertilizer," Journal of Bacteriology & Mycology: Open Access, vol. 6, pp. 274-278, 2018. Available at: https://doi.org/10.15406/jbmoa.2018.06.00217.

[17]        S. K. Sethi and S. P. Adhikary, "Azotobacter: A plant growth-promoting rhizobacteria used as biofertilizer," Dynamic Biochemistry, Process Biotechnology and Molecular Biology, vol. 6, pp. 68-74, 2012.

[18]        S. S. Porter, R. Bantay, C. A. Friel, A. Garoutte, K. Gdanetz, K. Ibarreta, B. M. Moore, P. Shetty, E. Siler, and M. L. Friesen, "Beneficial microbes ameliorate abiotic and biotic sources of stress on plants," Functional Ecology, vol. 34, pp. 2075-2086, 2020. Available at: https://doi.org/10.1111/1365-2435.13499.

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Funding:

This research is funded by Laboratory of Soil Biology, Faculty of Agriculture, Universitas Padjadjaran, Indonesia.

Competing Interests:

The authors declare that they have no competing interests.

Acknowledgement:

Authors thank sugar factory of Jatitujuh in Majalengka, West Java to provide the vermicompost

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