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Current Research in Agricultural Sciences

June 2017, Volume 4, 2, pp 51-60

Molecular Characterization of Parental Lines of Rice Aiming to Address High Yield and Nutritional Quality Under Drought and Cold Stress Condition

Nomita Das


Partha S. Biswas

Nomita Das 1
Partha S. Biswas 2

  1. Jahangirnagar University, Savar, Dhaka, Bangladesh 1

  2. Bangladesh Rice Research Institute, Gazipur, Bangladesh 2

Pages: 51-60

DOI: 10.18488/journal.68.2017.42.51.60

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Article History:

Received: 02 March, 2017
Revised: 30 March, 2017
Accepted: 03 May, 2017
Published: 07 June, 2017


Abiotic stresses limit crop growth at different growth stages resulting low yield of rice. Molecular characterization of parental materials gives precise information on the extent of genetic diversity exists between them. A set of 60 SSRs randomly distributed over 12 chromosomes were used to analyze eight cultivars intend to be used as parent in breeding programs to address cold and drought tolerance, and nutritional quality of rice. A total of 300 alleles were detected across the cultivars for 51 polymophic markers with 5.88 alleles per loci. On average 30.6% of the genotypes shared a common allele at any given locus. UPGMA cluster analysis showed that 67% common alleles were shared by the cultivars. The cultivars were clearly grouped into two distinct clusters at 67.0% genetic similarity. Hbj. BVI showing high tolerance to cold stress at seedling stage differed from cold susceptible BR1, BRRI dhan28 and BRRI dhan29 by 33% alleles while BR18 that showed moderately cold tolerance differed by 29.0 - 29.9% alleles which indicated that only 3-4% alleles difference caused higher cold tolerance in Hbj. BVI. The moderate genetic distance between cold tolerant Hbj. BVI and high yielding BRRI dhan28 and BRRI dhan29 indicated that there is higher possibility of obtaining high yielding cold tolerant segregants from the crosses between them. On the other hand,  Kalobokri, which had 31.7 mg zinc a kilogram of polished rice differed by 33.0% alleles from the drought tolerant rainfed low varieties BRRI dhan56 and BRRI dhan57 having moderate level of zinc (~20 mg/kg), which also indicated that crosses between them might produce progenies with higher nutritional quality under drought environment.

Contribution/ Originality
This study is one of the few studies which have investigated the genetic distance between the parental lines that are intended to be used in the breeding program to address abiotic stresses, like drought and cold tolerance with high yield potential and enhanced nutritional quality, particularly zinc content in rice.  


Drought, Cold, Grain zinc content, Genetic diversity, SSR, Rice.



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This study received no specific financial support.

Competing Interests:

The authors declare that they have no competing interests.


We acknowledge Bangladesh Rice Research Institute for providing laboratory support for this study. We are also grateful to the scientists and staffs working in the molecular laboratory of Plant Breeding Division in BRRI. We express our gratitude to BRRI Ge

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( 146 ) Alleviating Climate Change Effect using Environmentally Friendly Process to Preserve Wood against Biodeteriaorating Agent
( 148 ) Management of Fusarium Wilt Disease of Tomato (Solanum lycopersicum L.) Using Selected Plant Extracts
( 149 ) Effect of Land Use and Slope Position on Infiltration Characteristics on Sandstone-Derived Soils of Southern Nigeria
( 150 ) Phytogeographic of Bromelia L. (Bromeliaceae) in the State Paraiba Brazil
( 151 ) A Survey of Wood Borer Apate terebrans Tunnels on Terminalia mantaly in Nigeria with Special Reference to the Niger Delta Region
( 152 ) Effect of Desiccation and Storage Environment on Longevity of Ehretia cymosa Thonn. Seeds
( 153 ) Factors Determining the Smallholder Milk Producers Participation in Contractual Agreements: The Case of North-West Bangladesh
( 155 ) Quality Characteristics of High-Oleic Sunflower Oil Extracted from Some Hybrids Cultivated Under Egyptian Conditions
( 156 ) Flaxseed Nutritied Meat Balls With High Antioxidant Potential
( 158 ) Effect of Integrated Application of Poultry Manure and Chemical NP Fertilizers on Growth, Yield and Yield Components of Highland Maize Variety on Vertisol at Ambo University on Station, Ethiopia
( 161 ) High-Sensitivity Testing of Effectiveness of Citrus Limon, Vitis Vinifera and Citrus Sinensis in the Postharvest Control of Callosobruchus Maculatus Fabricius (Coleoptera: Chrysomelidae) Infestation of Cowpea Seeds
( 162 ) Root Density, Distribution and Yield Relationships of High Yielding Sugarcane Varieties Under Sandy Soil Condition
( 163 ) Effect of Different Sources of Fertilizers on the Yield and Yield Components of Wheat Variety Kohat-2000 Under Rainfed Conditions of Kohat During 2012
( 164 ) GGE and Ammi Biplot Analysis for Field PEA Yield Stability in Snnpr State, Ethiopia
( 165 ) Effect of Different Types of Surfectants on Zinc Efficiency in Spinach Yield
( 166 ) Evaluation of Field Pea (Pisum Sativum L) Genotypes Performance for Yield and Yield Components at Five Growing Environments of Southern Ethiopia
( 168 ) Association between Yield Components of Sorghum (Sorghum Bicolor L. (Moench) Under Different Watering Intervals
( 169 ) Effect of Sowing Date on Maize Seed Yield and Quality: A Review
( 170 ) Comparative Growth Analysis and Yield Performance of Glycine Max under Jatropha Curcas Based Agrisilviculture System of Agroforestry in the Northern Part of Bangladesh
( 171 ) Correlation between Milk Yield, Somatic Cell Count and Milk Quality in Dairy Farming
( 173 ) Genotype X Environment Interaction and Stability Analysis for Yield and Yield Related Traits of Desi-Type Chickpea (Cicer Arietinum L.) In Ethiopia
( 174 ) Effect of Intra Spacing on Yield and Yield Components of Carrot (Daucus Carrota L.Sub Sp. Sativus)
( 175 ) Genotypic Difference in Growth and Yield Related Traits of Onion (Allium Cepa L.) Varieties at Southern Tigray
( 178 ) Evaluation of Different Tillage Practices on Growth and Yield of Fluted Pumpkin Telfairia Occidentalis in Uyo, Southeastern Nigeria
( 179 ) Heterosis for Nitrogen Fixation and Seed Yield and Yield Components in Chickpea (Cicer arietinum L.)
( 181 ) Harvesting Date Influences Cassava (Manihot Esculenta Crantz) Yield and Quality of Based-Products
( 182 ) Uncertainty Analysis and Calibration of Swat Model for Estimating Impacts of Conservation Methods on Streamflow and Sediment Yield in Thika River Catchment, Kenya
( 183 ) The Impact of Intensive Utilization of Cultivated Land on Grain Yields: A Case Study of Shandong Province in China
( 184 ) Genotype-Environment Interactions and Yield Stability of Cowpea (Vigna Unguiculata L. Walp) in Lomami Province, Central Part of Democratic Republic of Congo
( 185 ) Effect of Blended Fertilizer and Lime Application on Yield of Finger Millet, and Soil Properties of Acidic Soils in Western Ethiopia
( 186 ) Physiological Aspects of Yield Variation among Seven Groundnut Genotypes Cultivated under Rain-Fed Conditions
( 187 ) Effect of Planting Date on Growth and Yield of Bambara Groundnut (Vigna subtermnea (L) Verdc.) Varieties in Rainforest Zone of Delta State
( 190 ) Insect Activities and their Impact on the Yield of Abelmoschus esculentus L ( Malvaceae) in Bambili ( Mezam - Cameroon)
( 191 ) On-Farm Evaluation of Conservation Agriculture Practice on Weed Control and Yield of Wheat in Northern Bangladesh
( 192 ) Exogenous Application of Antioxidants on Leaf Chlorophyll, Yield Dynamics and Berry Quality of Sweet Pepper (Capsicum annuum L.)
( 193 ) Interactive Effects of Zinc-Arbuscular Mycorrhizal (AM) Fungi on Cadmium Uptake, Rubisco, Osmolyte Synthesis and Yield in Cajanus cajan (L.) Millsp.
( 194 ) Effect of Beneficial Soil Microbes on Growth and Yield of Celery in Volcanic Soil of West Java
( 195 ) Nutritional Properties of Some Novel Selected Fish Species in Khuzestan Province, Iran
( 197 ) Evaluation of Chemical-Nutritional Characteristics of Rainbow Trout Samples Affected by the