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Journal of Food Technology Research

June 2020, Volume 7, 1, pp 9-47

The Oils Refining Process and Contaminants in Edible Oils: A Review

Souleymane Zio


Hama Cisse


Oumarou Zongo


Flibert Guira


Francois Tapsoba


Namwin Siourime Somda


Fatoumata Hama-Ba


Laurencia Toulsoumde Songre-Ouattara


Cheikna Zongo


Yves Traore


Aly Savadogo

Souleymane Zio 1 Yves Traore 1 Aly Savadogo 1 ,
Hama Cisse 1 Oumarou Zongo 1 Flibert Guira 6
Francois Tapsoba 1 Namwin Siourime Somda 8

Fatoumata Hama-Ba 9 Laurencia Toulsoumde Songre-Ouattara 9 Cheikna Zongo 1 

  1. Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Burkina Faso. 1

  2. Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Burkina Faso; Gaoua Polytechnic University Center, Burkina Faso. 6

  3. Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Burkina Faso; Department of Food Technology, Research Institute of Applied Sciences and Technology, Burkina Faso. 8

  4. Department of Food Technology, Research Institute of Applied Sciences and Technology, Burkina Faso. 9

Pages: 9-47

DOI: 10.18488/journal.58.2020.71.9.47

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Article History:

Received: 28 October, 2019
Revised: 03 December, 2019
Accepted: 08 January, 2020
Published: 24 February, 2020


Edible oils are widely consumed foods. These oils come from various animal materials raw and vegetable products. Edible oils are prone to many contaminants. Contaminants can be found at all levels from oilseed production to conservation through refining processes and end up in oils. The contaminants origin may be of endogenous or exogenous. These are water, phosphorus, non-visible insoluble compounds, free fatty acids, residual hexane, benzo [a] pyrene, pesticides, dioxins, mycotoxins, mineral oils, cargo residues, minerals such as iron, copper, lead, gossypol, many primary and secondary oxidation products, etc. To eliminate or limit these compounds having a nuisance or toxicity for the consumer, it is allowed the refining of oils (chemical, physical or enzymatic). In addition, the regulatory limits of anti-nutritional factors in edible oils have been set in order to obtain quality oils and to guarantee the health of consumer living in developing country. This results in analytical methods developed for quantitative and qualitative evaluation of oils intended for human consumption.
Contribution/ Originality
This study on the contaminants of edible oils contributes to knowledge the sanitary consumption of these oils and their analytical methods. Previously, the contaminants in the oils did not experience infatuation. Our study focuses on pesticides, mycotoxins and gossypol which are anti-nutritional factors present in edible oils widely consumed.


Edible oil, Refining, Contaminant, Mycotoxin, Pesticide, Gossypol.


Abalaka, J., 1984. Aflatoxin distribution in edible oil-extracting plants and in poultry feedmills. Food and Chemical Toxicology, 22(6): 461-463.Available at:

Abalaka, J.A. and J.A. Elegbede, 1982. Aflatoxin distribution and total microbial counts in an edible oil extracting plant. I. Preliminary observations. Food and Chemical Toxicology, 20(1): 43-46.Available at:

Abalo, A., 2017. Traditional processing and marketing of palm oil. European University Editions. Available from

Abrunhosa, L., H. Morales, C. Soares, T. Calado, A.S. Vila-Chã, M. Pereira and A. Venâncio, 2016. A review of mycotoxins in food and feed products in Portugal and estimation of probable daily intakes. Critical Reviews in Food Science and Nutrition, 56(2): 249-265.Available at:

Adams, R., T. Geissman and J. Edwards, 1960. Gossypol, a pigment of cottonseed. Chemical Reviews, 60(6): 555-574.Available at:

Addis, P. and G. Warner, 1991. The potential health aspects of lipid oxidation products in food. Free Radicals and Food Additives: 77-119. Available from

Addis, P.B., 1986. Occurrence of lipid oxidation products in foods. Food and Chemical Toxicology, 24(10-11): 1021-1030.Available at:

AFSSA (French Food Safety Agency), 2009. Risk assessment linked to the presence of mycotoxins in the human and animal food chains. Final Report.

Afzali, D., M. Ghanbarian, A. Mostafavi, T. Shamspur and S. Ghaseminezhad, 2012. A novel method for high preconcentration of ultra trace amounts of B1, B2, G1 and G2 aflatoxins in edible oils by dispersive liquid–liquid microextraction after immunoaffinity column clean-up. Journal of Chromatography A, 1247: 35-41.Available at:

Agarwal, D.K., P. Singh, M. Chakrabarty, A.J. Shaikh and S.G. Gayal, 2003. Cottonseed oil quality, utilization and processing. Available from

Ali, R.F., 2010. Improvement the stability of fried sunflower oil by using different levels of pomposia (Syzyygium cumini). Electronic Journal of Environmental, Agricultural and Food Chemistry, 9(2): 396-403.

Alshannaq, A. and J.H. Yu, 2017. Occurrence, toxicity, and analysis of major mycotoxins in food. International Journal of Environmental Research and Public Health, 14(6): 1-20.Available at:

Amini, A. and F. Kamkar, 2005. The effects of gossypol on spermatogenesis in NMRI mice. Iranian Journal of Science and Technology (Sciences), 29(1): 123-133.

Anjorin, S., H. Makun and H. Iheneacho, 2008. Effect of Lippia Multiflora leaf extract and aspergillus flavus on germination and vigour indices of sorghum Bicolor [L](Moench). International Journal of Tropical Agriculture and Food System, 2(1): 130-134.

Anon, 1989. IARC monographs on the evaluation of carcinogenic risks to humans: Diesel and engine exhausts and some Nitroarenes. IARC Monogr Eval Carcinog Risks Hum, 33(3): 174.Available at:

ANSES, 2016. Opinion of the national agency for food safety, environment, and labor relating to the analysis of monitoring and control plans for undesirable substances in animal feed. Available from

Asmellash, S. and E. Dagne, 1989. Gossypol levels in cottonseed oil and cottonseed cake produced in Ethiopia. Bulletin of the Chemical Society of Ethiopia, 3(1): 51-55.

Bahrami, R., Y. Shahbazi and Z. Nikousefat, 2016. Occurrence and seasonal variation of aflatoxin in dairy cow feed with estimation of aflatoxin M 1 in milk from Iran. Food and Agricultural Immunology, 27(3): 388-400.Available at:

Bailey, A.E., 1948. Cottonseed and cottonseed products. Their chemistry and chemical technology. Cottonseed and cottonseed products. Their chemistry and chemical technology. New York: Interscience Publishers.

Bailey, C.A., R.D. Stipanovic, M.S. Ziehr, A.U. Haq, M. Sattar, L.F. Kubena, H.L. Kim and R. de M. Vieira, 2000. Cottonseed with a high (+)-to (−)-gossypol enantiomer ratio favorable to broiler production. Journal of Agricultural and Food chemistry, 48(11): 5692-5695.Available at:

Baldi, I., P. Lebailly, B. Mohammed-Brahim, L. Letenneur, J.F. Dartigues and P. Brochard, 2003. Neurodegenerative diseases and exposure to pesticides in the elderly. American Journal of Epidemiology, 157(5): 409-414.Available at:

Band, V., A.P. Hoffer, H. Band, A.E. Rhinehardt, R.C. Knapp, S.A. Matlin and D.J. Anderson, 1989. Antiproliferative effect of gossypol and its optical isomers on human reproductive cancer cell lines. Gynecologic Oncology, 32(3): 273-277.Available at:

Bao, L., M.W. Trucksess and K.D. White, 2010. Determination of aflatoxins B1, B2, G1, and G2 in olive oil, peanut oil, and sesame oil. Journal of AOAC International, 93(3): 936-942.

Bauer, W.J., R. Badoud and J. Löliger, 2010. Food science and technology: Principles of constituent chemistry and process technology. PPUR Polytechnic Presses. Available from

Benabid, H., 2009. Characterization of Algerian olive oil contributions of chemometric methods. Algeria: INATAA. Mentouri University of Constantine.

Benjelloun, N., 2014. Argan oil: Pharmacological properties and implementation of a control process. Doctoral Thesis.

Bennett, J.W. and M. Klich, 2003. Mycotoxins. Clinical Microbiology Review, 16: 497-516.Available at:

Benz, C.C., M.A. Keniry, J.M. Ford, A.J. Townsend, F.W. Cox, S. Palayoor, S.A. Matlin, W.N. Hait and K.H. Cowan, 1990. Biochemical correlates of the antitumor and antimitochondrial properties of gossypol enantiomers. Molecular Pharmacology, 37(6): 840-847.

Benzine, M., 2006. Pesticides: Toxicity, residues and analysis. Laboratory Technologies, 1. Available from

Berthiller, F., C. Hametner, P. Krenn, W. Schweiger, R. Ludwig, G. Adam, R. Krska and R. Schuhmacher, 2009. Preparation and characterization of the conjugated Fusarium mycotoxins zearalenone-4 O-β-D-glucopyranoside, α-zearalenol-4 O-β-D-glucopyranoside and β-zearalenol-4 O-β-D-glucopyranoside by MS/MS and two-dimensional NMR. Food Additives and Contaminants, 26(2): 207-213.Available at:

Betina, V., 1994. Bioactive secondary metabolite of microorganisms. Bibliographical Bases Pascal and Francis, 30: 468.

Birnin-Yauri, U.A. and S. Garba, 2011. Comparative studies on some physicochemical properties of baobab, vegetable, peanut and palm oils. Nigerian Journal of Basic and Applied Sciences, 19(1): 64- 67.Available at:

Blackstaffe, L., M. Shelley and R. Fish, 1997. Cytotoxicity of gossypol enantiomers and its quinone metabolite gossypolone in melanoma cell lines. Melanoma Research, 7(5): 364-372.Available at:

Boland, J., I. Koomen, J. van Lidth De Jeude and J. Oudejans, 2004. Pesticides: Composition, use and risks. Wageningen: Agromisa Foundation.

Bordin, K., M.M. Sawada, C.E. Da Costa Rodrigues, C.R. Da Fonseca and C.A.F. Oliveira, 2014. Incidence of aflatoxins in oil seeds and possible transfer to oil: A review. Food Engineering Reviews, 6(1-2): 20-28.Available at:

Boune, M.V.S., 2019. Valorization in organic synthesis of phytoconstituents (gossypol derivatives) extracted from cienfuegosia digitata. Cav. Doctoral Thesis, University of Normandy and the University of Nouakchott.

Bouvier, G., 2005. Contribution to the evaluation of the exposure of the population of Ile-de-France to pesticides. Doctoral Thesis, René Descartes-Paris V University, France.

Bové, F.J., 1970. The history of ergot. In the story of Ergot. Karger Publishers. pp: 134-165. Available from

Brink, M. and E.G. Achigan-Dako, 2012. Fiber plants. PROTA. Available from,+M.+and+E.G.+Achigan Dako,+2012.+Fiber+plants.+PROTA.&ots=eXY5r3HYp0&sig=ZxSIDJ8v6Bt5iiyS5MrSV_UvVRs#v=onepage&q=Brink%2C%20M.%20and%20E.G.%20Achigan-Dako%2C%202012.%20Fiber%20plants.%20PROTA.&f=false.

Brochard, G. and L.C. Bâcle, 2009. Mycotoxin in the workplace. Documents for the Occupational Physician, 119: 299-323.

Cai, Y., H. Zhang, Y. Zeng, J. Mo, J. Bao, C. Miao, J. Bai, F. Yan and F. Chen, 2004. An optimized gossypol high-performance liquid chromatography assay and its application in evaluation of different gland genotypes of cotton. Journal of Biosciences, 29(1): 67-71.Available at:

Cairns-Fuller, V., D. Aldred and N. Magan, 2005. Water, temperature and gas composition interactions affect growth and ochratoxin a production by isolates of penicillium verrucosum on wheat grain. Journal of Applied Microbiology, 99(5): 1215-1221.Available at:

Camard, J.P. and C. Magdelaine, 2001. Phytosanitary products: risks for the environment and health, knowledge of uses in non-agricultural areas. IAU IdF / ORS: 58. Available from

Camin, F., R. Larcher, M. Perini, L. Bontempo, D. Bertoldi, G. Gagliano, G. Nicolini and G. Versini, 2010. Characterization of authentic Italian extra-virgin olive oils by stable isotope ratios of C, O and H and mineral composition. Food Chemistry, 118(4): 901-909.Available at:

Canella, M. and G. Sodini, 1977. Extraction of gossypol and oligosaccharides from oilseed meals. Journal of Food Science, 42(5): 1218-1219.Available at:

Casas, J.S., C.D.M. Gordillo, E.O. Bueno, J.M. Expósito, M.F. Mendoza, T.A. Hierro, L.G. Gonzalez and M.M. Cano, 2009. Characteristics of virgin olive oils from the olive zone of extremadura (spain), and an approximation to their varietal origin. Journal of the American Oil Chemists' Society, 86(10): 933-940.Available at:

Cass, Q.B., E. Tiritan, S.A. Matlin and E.C. Freire, 1991. Gossypol enantiomer ratios in cotton seeds. Phytochemistry, 30(8): 2655-2657.Available at:

Catherine, R.R., 2014. Plant protection products: Innovation and security for sustainable agriculture. Lavoisier. Available from

Cavaliere, C., P. Foglia, C. Guarino, M. Nazzari, R. Samperi and A. Lagana, 2007. Determination of aflatoxins in olive oil by liquid chromatography–tandem mass spectrometry. Analytica Chimica Acta, 596(1): 141-148.Available at:

CEC, 2006. Commission Regulation (EC) No 1881/2006 of 19 December 2006 fixing maximum levels for certain contaminants in food. Text with EEA relevance. Available from

Chandler, D., A.S. Bailey, G.M. Tatchell, G. Davidson, J. Greaves and W.P. Grant, 2011. The development, regulation and use of biopesticides for integrated pest management. Philosophical Transactions of the Royal Society B: Biological Sciences, 366(1573): 1987-1998.

Chau, N.T.T., 2008. Total racemic synthesis of structural analogues of gossypol and apogossypol involving aromatic metalation reactions. Doctoral Thesis, University of Maine, France.

Chen, S., X. Yu, X. He, D. Xie, Y. Fan and J. Peng, 2009. Simplified pesticide multiresidues analysis in fish by low-temperature cleanup and solid-phase extraction coupled with gas chromatography/mass spectrometry. Food Chemistry, 113(4): 1297-1300.Available at:

Cherry, J.P. and M.S. Gray, 1981. Methylene chloride extraction of gossypol from cottonseed products. Journal of Food Science, 46(6): 1726-1733.Available at:

Chulze, S.N., 2010. Strategies to reduce mycotoxin levels in corn during storage: A review. Food Additives and Contaminants, 27(5): 651–657.Available at:

Codex, A., 1999. Standard for vegetable oils with a specific name. Codex-stan 210-1999. Adopted 1999. Revisions 2001, 2003, 2009. Amended 2005, 2011, 2013. 1-17. Available from

Codex, A., 2003. Maximum residue limits for processed or ready-to-eat products intended for human and animal consumption. Thirty-Fifth Session Rotterdam (Netherlands), March 13-April 5, 2003. pp: 30.

Compaoré, H., H. Sawadogo-Lingani, S. Samandoulougou, F. Guira, A. Savadogo and A.S. Traore, 2017. Ability of three mold strains to produce extracellular enzymes in solid medium in Burkina Faso. Journal of Applied Biosciences, 110(1): 10776-10782.Available at:

Corbett, J.R., K. Wright and A.C. Baillie, 1984. Compounds interfering with cell division. The biochemical mode of action of pesticides. 2nd Edn., London: Academic Press, 202.

Coumoul, X., 2016. Food contaminants and the risk of cancer. Nutrition and Dietetics Notebooks, 51(2): 104-110.Available at:

Couteux, A. and C. Salaün, 2009. Acta phytosanitary index. 45th Edn.: MAME. Retrieved from:

Creppy, E.E., F.C. Stormer, R. Röschenthaler and G. Dirheimer, 1983. Effects of two metabolites of ochratoxin A, (4R)-4-hydroxyochratoxin A and ochratoxin alpha, on immune response in mice. Infection and Immunity, 39(3): 1015-1018.Available at:

CSP, 2018. Global list of pesticides authorized by the Sahelian Pesticides Committee: 47. Available from

Cubero-Leon, E., R. Penalver and A. Maquet, 2014. Review on metabolomics for food authentication. Food Research International, 60: 95-107.Available at:

Cuvelier, M.E. and M.N. Maillard, 2012. Stability of edible oils during storage. Oilseeds, Fats, Lipids, 19(2): 125-132.Available at:

D'Mello, J., 2004. Contaminants and toxins in animal feeds. FAO Animal Production and Health Paper: 107-128. Available from

D'Mello, J.P.F., J.K. Porter and C.M. Placinta, 1997. Fusarium mycotoxins. In: D'MELLO, J.P.F. (Ed.), Handbook of Plant and Fungal Toxicants. Boca Raton, FL: CRC Press. pp: 287-301.

Dahlke, K., 1998. An enzymatic process for the physical refining of seed oils. Chemical Engineering & Technology: Industrial Chemistry-Plant Equipment-Process Engineering-Biotechnology, 21(3): 278-281.Available at:<278::aid-ceat278>;2-9.

Damaty, S.M. and B.J. Hudson, 1975. Preparation of low-gossypol cottonseed flour. Journal of the Science of Food and Agriculture, 26(1): 109-115.Available at:

Daniels, J.L., A.F. Olshan and D.A. Savitz, 1997. Pesticides and childhood cancers. Environmental Health Perspectives, 105(10): 1068-1077.

Dao, V.T., 2002. Gossypol and its new derivatives: Synthesis and study of biological activities. Doctoral Thesis, University of Paris Sud-Paris XI, France.

De Kock, J., W. De Greyt, V. Gibon and M. Kellens, 2005. Recent developments in refining and modifications: Elimination of contaminants in edible oils and reduction in the level of trans fatty acids. Oilseeds, Fats, Fat, 12(5-6): 378-384.Available at:

Deravel, J., F. Krier and P. Jacques, 2013. Biopesticides, supplements and alternatives to chemical plant protection products (bibliographic summary). Biotechnology, Agronomy, Society and Environment, 18(2): 220-232.

Dewailly, É., P. Ayotte, S. Bruneau, S. Gingras, M. Belles-Isles and R. Roy, 2000. Susceptibility to infections and immune status in inuit infants exposed to organochlorines. Environmental Health Perspectives, 108(3): 205-211.Available at:

DGAL, 2000. Data on the exposure of the French population to pesticide, lead, cadmium, arsenic and radionuclide residues from food. Our food; 24. Available from

Di Renzo, L., P. Gualtieri, L. Romano, G. Marrone, A. Noce, A. Pujia, M.A. Perrone, V. Aiello, C. Colica and A. De Lorenzo, 2019. Role of personalized nutrition in chronic-degenerative diseases. Nutrients, 11(8): 1-24.Available at:

Diaw, M.T., A. Dieng, G. Mergeai and J.L. Hornick, 2011. Cottonseed co-products in feed for broilers. In Annals of Veterinary Medicine, 155: 61-82.

Dieme, E., R. Fall, I. Sarr, F. Sarr, D. Traore and M. Seydi, 2016. Aflatoxin contamination of cereals in Africa: Review of existing control methods. International Journal of Biological and Chemical Sciences, 10(5): 2285-2299.Available at:

Dijkstra, A.J., 1998. Degumming revisited. Oleaginous Corps Gras Lipids, 5(5): 367-370.Available at: https://doi:10.1007/978-1-4614-3351-4.

Dikshith, T., S. Kumar, G. Tandon, R. Raizada, P. Ray, J. Woodrow, J. Wong, J. Seiber, A. Bimurzaev and G. Nikonov, 1989. Pesticide residues in edible oils and oil seeds. Bulletin of Environmental Contamination and Toxicology, 42(1): 50-56.Available at:

Diop, A., 2013. Diagnosis of pesticide use practices and quantification in the Niayes area of Dakar (Senegal). Littoral. Available from file:///C:/Users/nghf/Downloads/DIOPAmadou%20(4).pdf.

Diouf, F.B.H., 2015. Analysis of the genetic determinism of the inhibition of gossypol synthesis in the seed in the progeny of the hybrid [(Gossypium hirsutum L. x G. raimondii Ulb.) ² x G. sturtianum Willis]. Doctoral Thesis, University of Liège, Belgium.

Dodou, K., 2005. Investigations on gossypol: Past and present developments. Expert Opinion on Investigational Drugs, 14(1): 1419-1434.Available at:

EFSA (European Food Safety Authority), 2008. Scientific opinion of the panel on contaminants in the food chain on a request from the European Commission on gossypol as undesirable substance in animal feed. The EFSA Journal, 908: 1-55.

Elzupir, A.O., M.A. Suliman, I.A. Ibrahim, M.H. Fadul and A.M. Elhussein, 2010. Aflatoxins levels in vegetable oils in Khartoum State, Sudan. Mycotoxin Research, 26(2): 69-73.Available at:

Engel, E., M. Meurillon, C. Planche and P. Peyret, 2014. Fate of toxic food contaminants in the digestive environment. Agronomic Innovations, 36: 135-149.

Escobar, J., S. Lorán, I. Giménez, E. Ferruz, M. Herrera, A. Herrera and A. Ariño, 2013. Occurrence and exposure assessment of fusarium mycotoxins in maize germ, refined corn oil and margarine. Food and Chemical Toxicology, 62: 514-520.Available at:

Evrard, J., X.P.X. Pages, C. Argenson and O. Morin, 2007. Processes for obtaining and nutritional compositions of sunflower, olive and rapeseed oils. Nutrition and Diet Books, 1(42): 13-23.Available at:

Fanelli, C. and A.A. Fabbri, 1989. Relationship between lipids and aflatoxin biosynthesis. Mycopathologia, 107(2-3): 115-120.Available at: https://doi:10.1007/BF00707547.

Fangeat, L., 2008. Mycotoxins in cattle. Doctoral Dissertation, Thesis Presented at Claude University.

FAO, 1986. International code of conduct on the distribution and use of pesticides. Rome: Food and Agriculture Organization of the United Nations. pp: 28

FAO, 1993. Food codex commission: Codex standards for fats, oils and derived products. Rome: CAC, 8.

Farhoosh, R. and K.R. Esmaeilzadeh, 2009. Anti-rancidity effects of sesame and rice bran oils on canola oil during deep frying. Journal of the American Oil Chemists' Society, 86(6): 539-544.Available at:

Fellinger, A., 2006. Worldwide mycotoxin regulations and analytical challenges. World Grain Summit: Foods and Beverages: 17-20.

Fenik, J., M. Tankiewicz and M. Biziuk, 2011. Properties and determination of pesticides in fruits and vegetables. Trends in Analytical Chemistry, 30(6): 814-826.Available at:

Ferrer, C., M.J. Gómez, J.F. García-Reyes, I. Ferrer, E.M. Thurman and A.R. Fernández-Alba, 2005. Determination of pesticide residues in olives and olive oil by matrix solid-phase dispersion followed by gas chromatography/mass spectrometry and liquid chromatography/tandem mass spectrometry. Journal of Chromatography A, 1069(2): 83-194.Available at:

Fillatre, Y., 2011. Phytosanitary products: Development of a multi-residue analysis method in essential oils by coupling liquid chromatography with mass spectrometry in tandem mode. Doctoral Thesis, University of Angers.

Fine, F., C. Brochet, M. Gaud, P. Carre, N. Simon, F. Ramli and F. Joffre, 2016. Micronutrients in vegetable oils: The impact of crushing and refining processes on vitamins and antioxidants in sunflower, rapeseed, and soybean oils. European Journal of Lipid Science and Technology, 118(5): 680-697.Available at:

Fink-Gremmels, J., 2008. Mycotoxins in cattle feeds and carry-over to dairy milk: A review. Food Additives and Contaminants, 25(2): 172-180.Available at:

Firmin, S., 2011. Detoxification efficiency of aflatoxin B1 and ochratoxin A by an organic adsorbent: evaluation by excretion balance and toxicokinetic parameters in rats and dairy sheep. Doctoral Thesis, Blaise Pascal University - Clermont-Ferrand II, NNT.

Frankel, E.N., 1989. The antioxidant and nutritional effects of tocopherols, ascorbic acid and beta-carotene in relation to processing of edible oils. Library Nourishment and Diet(43): 297-312.Available at:

Fuentes, E., M.E. Báez and A. Quiñones, 2008. Suitability of microwave-assisted extraction coupled with solid-phase extraction for organophosphorus pesticide determination in olive oil. Journal of Chromatography A, 1207(1-2): 38-45.Available at:

Gamboa, D.A., M.C. Calhoun, S.W. Kuhlmann, A.U. Haq and C.A. Bailey, 2001a. Use of expander cottonseed meal in broiler diets formulated on a digestible amino acid basis. Poultry science, 80(6): 789-794.Available at:

Gamboa, D.A., M.C. Calhoun, S.W. Kuhlmann, A.U. Haq and C.A. Bailey, 2001b. Tissue distribution of gossypol enantiomers in broilers fed various cottonseed meals. Poultry Science, 80(7): 920-925.

Garcia-Reyes, J.F., C. Ferrer, M.J. Gómez-Ramos, A.R. Fernández-Alba and A. Molina-Díaz, 2007. Determination of pesticide residues in olive oil and olives. Trac Trends in Analytical Chemistry, 26(3): 239-251.

Gauthier, A., 2016. Mycotoxins in food and their impact on health PhD. Thesis, University of Bordeaux, Bordeaux: France. Available from

Genot, C. and M.C. Michalski, 2010. Metabolic impact of lipid structures and oxidation in food. Agronomic Innovations, 10: 43-67.

Gerrit, V.D., 2008. Industrial experiences with pesticide removal during edible oil refining. European Journal of Lipid Science and Technology, 110(11): 982-989.Available at:

Gibon, V. and A. Tirtiaux, 1998. Un raffinage SOFT®: Innovations technologiques. OCL. Oléagineux, Corps Gras, Lipides, 5(5): 371-377.

Greenlee, A.R., T.E. Arbuckle and P.H. Chyou, 2003. Risk factors for female infertility in an agricultural region. Epidemiology, 14(4): 429-436.Available at:

Gromadzka, J. and W. Wardencki, 2011. Trends in edible vegetable oils analysis. Part A. Determination of different components of edible oils-a review. Polish Journal of Food and Nutrition Sciences, 61(1): 33-43.Available at:

Guardia, R.M., A. Ruiz Medina, D.A. Molina and C.M.J. Ayora, 2006. Influence of harvesting method and washing on the presence of pesticide residues in olives and olive oil. Journal of Agricultural and Food chemistry, 54(22): 8538-8544.Available at:

Guéguen, M., J.C. Amiard, N. Arnich, P.M. Badot, D. Claisse, T. Guérin and J.P. Vernoux, 2011. Shellfish and residual chemical contaminants: Hazards, monitoring, and health risk assessment along French coasts. Available from

Guerre, P., 1998. Main mycotoxicoses observed in ruminants. The Veterinary Point, 29: 1231-1238.

Guezlane-Tebibel, N., N. Bouras and M. Ould El Hadj, 2016. Mycotoxins: A public health danger. Algerian Journal of Arid Environment, 6(1).

Hadjeba-Medjdoub, K., 2012. Risk of multi-contamination with mycotoxins and means of deactivation by the walls of yeasts and yeasts enriched in glutathione or seleno-methionine. Doctoral Thesis.

Halliwell, B. and J.C. Gutteridge, 1984. Lipid peroxidation, oxygen radicals, cell damage, and antioxidant therapy. The Lancet, 323(8391): 1396-1397.Available at:

Hanny, B.W., 1980. Gossypol, flavonoid, and condensed tannin content of cream and yellow anthers of five cotton (Gossypium hirsutum L.) cultivars. Journal of Agricultural and Food Chemistry, 28(3): 504-506.Available at:

Harris, B. and C.R. Staples, 1992. The problems of mycotoxins in dairy cattle rations. Cooperative Extension Service, University of Florida, Institute of Food and Agricultural Sciences. Available from

Hassall, K.A., 1982. The chemistry of pesticides: Their metabolism, mode of action and uses in crop protection. Macmillan. Available from

He, Z., Y. Wang, L. Wang, Y. Peng, W. Wang and X. Liu, 2017. Determination of 255 pesticides in edible vegetable oils using QuEChERS method and gas chromatography tandem mass spectrometry. Analytical and Bioanalytical Chemistry, 409(4): 1017-1030.Available at:

Henry, M., G. Pesti and T. Brown, 2001. Pathology and histopathology of gossypol toxicity in broiler chicks. Avian Diseases, 45(3): 598-604.Available at:

Hernández-Borges, J., J.C. Cabrera, M.A. Rodríguez-Delgado, E.M. Hernández-Suárez and V.G. Saúco, 2009. Analysis of pesticide residues in bananas harvested in the Canary Islands (Spain). Food Chemistry, 113(1): 313-319.Available at:

Heussner, A.H. and L.E. Bingle, 2015. Comparative ochratoxin toxicity: A review of the available data. Toxins, 7(10): 4253-4282.Available at:

Hodgson, E. and P.E. Levi, 1996. Pesticides: An important but underused model for the environmental health sciences. Environmental Health Perspectives, 104(suppl 1): 97-106.Available at:

Holbrook, C.C., B.Z. Guo, D.M. Wilson and C. Kvien, 2004. Effect of drought tolerance on preharvest aflatoxin contamination in peanut. Proceedings of the 4th International Crop Science Congress Brisbane, Australia.

Huang, L., Y.-K. Si, G. Snatzke, D.-K. Zheng and J. Zhou, 1988. Absolute configuration of gossypol. Collection of Czechoslovak Chemical Communications, 53(11): 2664-2666.Available at:

Hui, Y.H., 1996. Bailey's industrial oil and fat products. Edible Oil and Fat Products Oils and Oil Seeds (No. L-0332). John Wiley and Sons, 2.

Huybrechts, B., E.K. Tangni, P. Debongnie, J. Geys and A. Callebaut, 2013. Analytical methods for the determination of mycotoxins in agricultural products: A review. Cahiers Agricultures, 22(3): 202-215.Available at:

Idris, Y.M., A.A. Mariod, I.A. Elnour and A.A. Mohamed, 2010. Determination of aflatoxin levels in Sudanese edible oils. Food and Chemical Toxicology, 48(8-9): 2539-2541.Available at:

International Agency for Research on Cancer, 1993. Monographs on the evaluation of carcinogenic risks to humans: Heterocyclic amines and mycotoxins. Lyon, France: IARC, 56.

Iterg, 2019. The technology of fatty substances. Available from

Jaroszewski, J.W., 1998. Compound from the cotton plant. In Cotton. Berlin, Heidelberg: Springer. pp: 335-349.

Jayaraj, R., P. Megha and P. Sreedev, 2016. Organochlorine pesticides, their toxic effects on living organisms and their fate in the environment. Interdisciplinary Toxicology, 9(3-4): 90-100.Available at:

Jayashree, T. and C. Subramanyam, 2000. Oxidative stress as a prerequisite for aflatoxin production by aspergillus parasiticus. Free Radical Biology and Medicine, 29(10): 981-985.Available at:

Joseph, A., S. Matlin and P. Knox, 1986. Cytotoxicity of enantiomers of gossypol. British Journal of Cancer, 54: 511-513.Available at:

Jouve, J.L., 1993. The microbiological quality of food. Mastery and criteria. Polytechnica, 7: 124-125.

Judde, A., 2004. Prevention of fatty acid oxidation in a cosmetic product: Mechanisms, consequences, means of measurement, which antioxidants for which applications? Oilseeds, Fat, Lipids, 11(6): 414-418.

Kamal-Eldin, A. and L.A. Appelqvist, 1996. Aldehydic acids in frying oils: Formation, toxicological significance and analysis. Fats and Oils, 47(5): 342-348.Available at:

Kamimura, H., M. Nishijima, S. Tabata, K. Yasuda, H. Ushiyama and T. Nishima, 1986. Survey of mycotoxins contamination in edible oil and fate of mycotoxins during oil-refining processes. Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi), 27(1): 59-63_51.Available at:

Kamimura, H., M. Nishijima, S. Tabata, K. Yasuda, H. Ushiyama and T. Nishima, 1986. Survey of mycotoxins contamination in edible oil and fate of mycotoxins during oil-refining processes. Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi), 27(1): 59 -63_51.

Kanoi, F., 2004. Agri-inputs marketing research. Cotton crop track. Cotton Crop Track, A Report on Seeds. Chennai, 2005.

Keller, S.E., T.M. Sullivan and S. Chirtel, 1997. Factors affecting the growth of Fusarium proliferatum and the production of fumonisin B 1: Oxygen and pH. Journal of Industrial Microbiology and Biotechnology, 19(4): 305-309.Available at:

Kim, H.J. and D.B. Min, 2008. Tocopherol stability and prooxidant mechanisms of oxidized tocopherols in lipids. Food Lipids: Chemistry, Nutrition, and Biotechnology, 3: 435-448.

Klere, J., 1992. History of fatty substances. Manual of fatty substances, Ed Lavoisier. Paris.

Koné, S., 2001. Modern small and very small scale oilseed processing units: Performance and limits. Info gate, gtz. 12. Available from

Krieger, R., 2001. Handbook of pesticide toxicology: Principles and agents. Academic Press. Available from

Kubow, S., 1990. Toxicity of dietary lipid peroxidation products. Trends in Food Science & Technology, 1: 67-71.Available at:

Kuiper-Goodman, T. and P.M. Scott, 1989. Risk assessment of the mycotoxin ochratoxin A. Biomedical and Environmental Sciences, 2(3): 179-248.

Lacey, J., 1991. Natural occurrence of mycotoxins in growing and conserved forage crops. Natural Occurrence of Mycotoxins in Growing and Conserved Forage Crops: 363-397. Available from

Lacoste, F., H. Lechat, X. Pages, J.N. Arnaud, B. E. Brenne, B. Soulet, C. Camisuli, S. Birot, J. Fazeuilh and J. Escabasse, 2005. Control of undesirable compounds in vegetable oils and establishment of observatories. Oilseeds, Fats, Fat, 12(5-6): 372-377. Available from

Laguerre, M., L.J. López-Giraldo, J. Lecomte, M. Pina and P. Villeneuve, 2007. Tools for in vitro evaluation of antioxidant capacity. Oilseeds, Fats, Lipids, 14(5): 278-292.

Lecerf, J.M., 2011. Vegetable oils: Particularities and usefulness: Vegetable oils: Particularities and usefulness. Medicine for Metabolic Diseases, 5(3): 257-262.Available at:

Leng, P., Z. Zhang, G. Pan and M. Zhao, 2011. Applications and development trends in biopesticides. African Journal of Biotechnology, 10(86): 19864-19873.Available at:

Lentza-Rizos, C., E. Avramides and F. Cherasco, 2001. Low-temperature clean-up method for the determination of organophosphorus insecticides in olive oil. Journal of Chromatography A, 912(1): 135-142.Available at:

Lerma-García, M.a.J.s., V. Concha-Herrera, J.M. Herrero-Martínez and E.F. Simó-Alfonso, 2009. Classification of extra virgin olive oils produced at la comunitat valenciana according to their genetic variety using sterol profiles established by high-performance liquid chromatography with mass spectrometry detection. Journal of Agricultural and Food Chemistry, 57(22): 10512-10517.Available at:

Li, L., Z. Zhou, C. Pan, C. Qian, S. Jiang and F. Liu, 2007. Determination of organophosphorus pesticides in soybean oil, peanut oil and sesame oil by low-temperature extraction and GC-FPD. Chromatographia, 66(7-8): 625-629.Available at:

Lindberg, M., R. Naqvi, S. Matlin, R. Zhou, G. Bialy and R. Blye, 1987. Comparative anti-fertility effects of gossypol enantiomers in male hamsters. International Journal of Andrology, 10(4): 619-623.Available at:

Liu, B., 1957. A modest proposal for the use of crude cottonseed oil for antifertility. Shanghai Journal of Traditional Chinese Medicine, 6: 43.

LNE, 2008. Pesticides. 15. Available from

Lordelo, M., A. Davis, M. Calhoun, M. Dowd and N. Dale, 2005. Relative toxicity of gossypol enantiomers in broilers. Poultry Science, 84(9): 1376-1382.Available at:

Louis, K., 2013. Synthesis and functionalization of aldehydes resulting from the cleavage of unsaturated fatty esters. Doctoral Thesis, University of Poitiers, France.

Luchese, R.H. and W. Harrigan, 1993. Biosynthesis of aflatoxin—the role of nutritional factors. Journal of Applied Bacteriology, 74(1): 5-14.Available at:

Lyman, C.M., B.P. Baliga and M.W. Slay, 1959. Reactions of proteins with gossypol. Archives of Biochemistry and Biophysics, 84(2): 486-497.Available at:

Maata, N., B. Kartah, H. Harhar, S. Gharby, E. Benazzouz, D. Guillaume and Z. Charrouf, 2011. Detection of adulteration of argan oil by virgin and refined vegetable oils. First International Congress on the Argan Tree. pp: 15-17.

Magan, N. and D. Aldred, 2005. Conditions of formation of ochratoxin A in drying, transport and in different commodities. Food Additives and Contaminants, 22(s1): 10-16.Available at:

Mahmoudi, R. and R. Norian, 2015. Aflatoxin B1 and M1 contamination in cow feeds and milk from Iran. Food and Agricultural Immunology, 26(1): 131-137.Available at:

Mannon, J. and E. Johnson, 1985. Fungi down on the farm. New Scientist, 105: 12-16.

Marasas, W.F.O., T.S. Kellerman, W.C. Gelderblom, P.G. Thiel, J.J. Van der Lugt and J.A. Coetzer, 1988. Leukoencephalomalacia in a horse induced by fumonisin B- isolated from fusarium moniliforme. Available from

Marin, S., A. Ramos, G. Cano-Sancho and V. Sanchis, 2013. Mycotoxins: Occurrence, toxicology, and exposure assessment. Food and Chemical Toxicology, 60: 218-237.Available at:

Markman, A.L. and V.P. Rzhekhin, 1969. Gossypol and its derivatives (Translated from Russian). Jerusalem: Israel Program for Scientific Translations.

Martin, J., A. Ba, P. Dimanche and R. Schilling, 1999. How to fight aflatoxin contamination of peanuts? Experiments conducted in Senegal. Agriculture and Development, 23: 58-67.

Matlin, S., R. Zhou, G. Bialy, R. Blye, R. Naqvi and M. Lindberg, 1985. (−)-gossypol: An active male antifertility agent. Contraception, 31(2): 141-149.Available at:

Mawussi, G., 2008. Environmental balance of the use of organochlorine pesticides in cotton, coffee and cocoa crops in Togo and search for alternatives by evaluating the insecticidal power of extracts of local plants against the bark beetle (Hypothenemus hampei Ferrari). (Doctoral Dissertation, INPT).

Mazet, A., 2005. Ecotoxicological and environmental study of the Drôme river: Application to the survival of the otter. Doctoral Thesis of the Joseph-Fourier-Grenoble I University.

Menegaux, F., A. Baruchel, Y. Bertrand, B. Lescoeur, G. Leverger, B. Nelken, D. Sommelet, D. Hémon and J. Clavel, 2006. Household exposure to pesticides and risk of childhood acute leukaemia. Occupational and Environmental Medicine, 63(2): 131-134.Available at:

Mirghani, M. and M.Y. Che, 2003. A new method for determining gossypol in cottonseed oil by FTIR spectroscopy. Journal of the American Oil Chemists' Society, 80(7): 625-628.Available at:

Monbaliu, S., C. Van Poucke, C.l. Detavernier, F. Dumoulin, M. Van De Velde, E. Schoeters, S. Van Dyck, O. Averkieva, C. Van Peteghem and S. De Saeger, 2010. Occurrence of mycotoxins in feed as analyzed by a multi-mycotoxin LC-MS/MS method. Journal of Agricultural and Food Chemistry, 58(1): 66-71.Available at:

Moreno-González, D., J.F. Huertas-Pérez, A.M. García-Campaña and L. Gámiz-Gracia, 2014. Determination of carbamates in edible vegetable oils by ultra-high performance liquid chromatography–tandem mass spectrometry using a new clean-up based on zirconia for QuEChERS methodology. Talanta, 128: 299-304.Available at:

Murphy, P.A., S. Hendrich, C. Landgren and C.M. Bryant, 2006. Food mycotoxins: An update. Journal of Food Science, 71(5): R51-R65.Available at:

Nabizadeh, S., S. Shoeibi, G. Jahed-Khaniki, R. Nabizadeh-Nodeh and E. Shokoohi, 2015. Validation of simultaneous analysis method for determination of aflatoxins in olive oil by high performance liquid chromatographyfluorescence detector. Journal of Food Safety and Hygiene, 1(2): 63-68.

National Cancer Institute, 2014. Pesticides and risks of cancer. State of knowledge as of April 2014: 12. Available from

Njobeh, P.B., M.F. Dutton and H.A. Makun, 2010. Mycotoxins and human health: Significance, prevention and control. In Smart Biomolecules in Medicine. VBRI Press India. pp: 132-177. Available from file:///C:/Users/nghf/Downloads/Chapter_6Njobehetal..pdf.

Nolen, G.A., J.C. Alexander and N.R. Artman, 1967. Long-term rat feeding study with used frying fats. The Journal of Nutrition, 93(3): 337-348.Available at:

Nomeir, A. and M. Abou-Donia, 1982. Gossypol: High-performance liquid chromatographic analysis and stability in various solvents. Journal of the American Oil Chemists' Society, 59(12): 546-549.Available at:

Nwanguma, B.C., A.C. Achebe, L.U.S. Ezeanyika and L.C. Eze, 1999. Toxicity of oxidized fats ii: Tissue levels of lipid peroxides in rats fed a thermally oxidized corn oil diet. Food and chemical Toxicology, 37(4): 413-416.Available at:

Nzikou, J.M., L. Matos, J.E. Moussounga, C.B. Ndangui, N.P. Pambou-Tobi, E.M. Bandzouzi, A. Kimbonguila, M. Linder and S. Desobry, 2009. Study of oxidative and thermal stability of vegetable oils during frying. Research Journal of Applied Science, 4(2): 94-100.

Oatley, J.T., M.D. Rarick, G.E. Ji and J.E. Linz, 2000. Binding of aflatoxin B1 to bifidobacteria in vitro. Journal of Food Protection, 63(8): 1133-1136.Available at:

Oliva, A., A. Spira and L. Multigner, 2001. Contribution of environmental factors to the risk of male infertility. Human Reproduction, 16(8): 1768-1776.Available at:

Oliver, C.L., M.B. Miranda, S. Shangary, S. Land, S. Wang and D.E. Johnson, 2005. (−)-Gossypol acts directly on the mitochondria to overcome Bcl-2-and Bcl-XL-mediated apoptosis resistance. Molecular Cancer Therapeutics, 4(1): 23-31.

Ollivier, D., 2003. Research on adulteration in vegetable oils: Application to the quality of virgin oils and in particular olive oil. Oilseeds, Fat, Lipids, 10(4): 315-320.

Pages, X., O. Morin, C. Birot, M. Gaud, S. Fazeuilh and M. Gouband, 2010. Refining of oils and fatty substances and elimination of contaminants. Oilseeds, Fats, Fats, 17(2): 86-99.

Panigrahi, S., V.E. Plumb and D.H. Machin, 1989. Effects of dietary cottonseed meal, with and without iron treatment, on laying hens. British Poultry Science, 30(3): 641-651.Available at:

Papachristou, A. and P. Markaki, 2004. Determination of ochratoxin A in virgin olive oils of Greek origin by immunoaffinity column clean-up and high-performance liquid chromatography. Food Additives and Contaminants, 21(1): 85-92.Available at:

Park, D., L. Lee, R. Price and A. Pohland, 1988. Review of the decontamination of aflatoxins by ammoniation: Current status and regulation. Journal-Association of official analytical chemists, 71(4): 685-703.

Parker, W.A. and D. Melnick, 1966. Absence of aflatoxin from refined vegetable oils. Journal of the American Oil Chemists Society, 43(11): 635-638.Available at:

Pearson, A.M., J.I. Gray, A.M. Wolzak and N.A. Horenstein, 1983. Safety implications of oxidized lipids in muscle foods. Food Technology (USA).

Peers, F.G. and C.A. Linsell, 1975. Aflatoxin contamination and its heat stability in Indian cooking oils. Tropical Science, 17(4): 229-232.

Pierides, M., H. El-Nezami, K. Peltonen, S. Salminen and J. Ahokas, 2000. Ability of dairy strains of lactic acid bacteria to bind aflatoxin M1 in a food model. Journal of Food Protection, 63(5): 645-650.Available at:

Pitt, J.I., 1996. What are mycotoxins? Australian Mycotoxin Newsletter, 7(4).

Polgár, L., B. Kmellár, J.F. García-Reyes and P. Fodor, 2012. Comprehensive evaluation of the clean-up step in QuEChERS procedure for the multi-residue determination of pesticides in different vegetable oils using LC-MS/MS. Analytical Methods, 4(4): 1142-1148.Available at:

Polsky, B., S.J. Segal, P.A. Baron, J.W. Gold, H. Ueno and D. Armstrong, 1989. Inactivation of human immunodeficiency virus in vitro by gossypol. Contraception, 39(6): 579-587.Available at:

Proctor, J., H. O’Neill, H. Reilich, R. Levi and W. Pons, 1968. Physiological evaluation of solvent-treated cottonseed meals in rations for laying hens. Journal of the American Oil Chemists’ Society, 45(5): 393-396.Available at:

Qi, X., 2010. Development of a matrix solid-phase dispersion-sonication extraction method for the determination of fungicides residues in ginseng extract. Food Chemistry, 121(3): 758-762.Available at:

Qian, S. and Z. Wang, 1984. Gossypol: A potential antifertility agent for males. Annual Review of Pharmacology and Toxicology, 24(1): 329-360.Available at:

Quin, L.D., 2000. A guide to organophosphorus chemistry. John Wiley and Sons. Available from:

Rahma, E.H. and M.N. Rao, 1984. Gossypol removal and functional properties of protein produced by extraction of glanded cottonseed with different solvents. Journal of Food Science, 49(4): 1057-1060.Available at:

Ravelo-Pérez, L.M., J. Hernández-Borges, T.M. Borges-Miquel and M.A. Rodríguez-Delgado, 2008. Solid-phase micro-extraction and sample stacking micellar electrokinetic chromatography for the analysis of pesticide residues in red wines. Food Chemistry, 111(3): 764-770.Available at:

Razzazi-Fazeli, E., B. Rabus, B. Cecon and J. Böhm, 2002. Simultaneous quantification of a-trichothecene mycotoxins in grains using liquid chromatography–atmospheric pressure chemical ionisation mass spectrometry. Journal of Chromatography A, 968(1-2): 129-142.Available at:

Reboux, G., 2006. Mycotoxins: Health effects and interactions with other organic components. French Review of Allergology and Clinical Immunology, 46(3): 208-212.Available at:

Repetto, R. and S.S. Baliga, 1996. Pesticides and the immune system: The public health risks. World Resources Institute.

Richard, J.L., G.A. Payne, A.E. Desjardins, C. Maragos, W. Norred and J. Pestka, 2003. Mycotoxins: Risks in plant, animal and human systems. CAST Task Force Report, 139: 101-103.

Riemersma, R.A., 2002. Analysis and possible significance of oxidized lipids in food. European Journal of Lipid Science and Technology, 104(7): 419-420.Available at:<419::aid-ejlt419>;2-s.

Robertson, A.E., 2005. Risk of aflatoxin contamination increases with hot and dry growing conditions. Available from

Roszko, M., A. Szterk, K. Szymczyk and B. Waszkiewicz-Robak, 2012. PAHs, PCBs, PBDEs and pesticides in cold-pressed vegetable oils. Journal of the American Oil Chemists' Society, 89(3): 389-400.Available at:

Sahay, S.S. and T. Prasad, 1990. The occurrence of aflatoxins in mustard and mustard products. Food Additives & Contaminants, 7(4): 509-513.Available at:

Samarajeewa, U., T. Gamage and S. Arseculeratne, 1983. Aflatoxin contamination of coconut oil from small scale mills: Toxin levels and their relation to free fatty acid content. Journal of the National Science Foundation of Sri Lanka, 11(2): 203 - 210.Available at:

Schmidely, P. and D. Sauvant, 2001. Butyrous rate and fat composition of milk in small ruminants: Effects of the intake of fat or concentrated feed. INRA Animal Production, 14(5): 337–354.

Scott, P.M., 1998. Industrial and farm detoxification processes for mycotoxins. Journal of Veterinary Medicine (France), 149: 543 548.

Scudamore, K.A. and C.T. Livesey, 1998. Occurrence and significance of mycotoxins in forage crops and silage: A review. Journal of the Science of Food and Agriculture, 77(1): 1-17.Available at:<1::aid-jsfa9>;2-4.

Sharmili, K., S. Jinap and R. Sukor, 2016. Development, optimization and validation of QuEChERS based liquid chromatography tandem mass spectrometry method for determination of multi-mycotoxin in vegetable oil. Food Control, 70: 152-160.Available at:

Sheen, L.Y., H.W. Chen, Y.L. Kung, C.T. Liu and C.K. Lii, 1999. Effects of garlic oil and its organosulfur compounds on the activities of hepatic drug-metabolizing and antioxidant enzymes in rats fed high-and low-fat diets. Nutrition and Cancer, 35(2): 160-166.Available at:

Shelley, M.D., L. Hartley, R.G. Fish, P. Groundwater, J.J.G. Morgan, D. Mort and A. Evans, 1999. Stereo-specific cytotoxic effects of gossypol enantiomers and gossypolone in tumour cell lines. Cancer Letters, 135(2): 171-180.Available at:

Silva, L., J. Pinto, J. Carrola and F. Paiva-Martins, 2010. Oxidative stability of olive oil after food processing and comparison with other vegetable oils. Food Chemistry, 121(4): 1177-1187.Available at:

Slope, S.L., S. Wu, Z. Wu, H. Shen and X.J. Chang, 2013. Patent CN 103205311B. A method for industrially removing zearalenone out of maize germ. State Grain Administration Institute. (Granted 24 Dec 2014).

Sobhanzadeh, E., N.K.A. Bakar, M.R.B. Abas and K. Nemati, 2011. An efficient extraction and clean-up procedure for pesticide determination in olive oil. European Journal of Lipid Science and Technology, 113(7): 862-869.Available at:

Spanjer, M.C., P.M. Rensen and J.M. Scholten, 2008. LC–MS/MS multi-method for mycotoxins after single extraction, with validation data for peanut, pistachio, wheat, maize, cornflakes, raisins and figs. Food Additives and Contaminants, 25(4): 472-489.Available at:

Srivastava, S., M. Siddiqui and T. Seth, 1983. Organochlorine pesticide residues in groundnut oil. Journal of Food Science and Technology, 20(1): 25-27.

Staprans, I., D.A. Hardman, X.M. Pan and K.R. Feingold, 1999. Effect of oxidized lipids in the diet on oxidized lipid levels in postprandial serum chylomicrons of diabetic patients. Diabetes Care, 22(2): 300-306.Available at:

Stoytcheva, M. and R. Zlatev, 2011. Organophosphorus pesticides analysis. Pesticides in the modern world-Trends in Pesticides Analysis (M. Stoytcheva, Ed.). InTech, Croacia. pp: 143-164. Available from

Sun, Y.E., W.D. Wang, H.W. Chen and C. Li, 2011. Autoxidation of unsaturated lipids in food emulsion. Critical Reviews in Food Science and Nutrition, 51(5): 453-466.Available at:

Sweeney, M.J. and A.D. Dobson, 1998. Mycotoxin production by aspergillus, fusarium and penicillium species. International Journal of Food Microbiology, 43(3): 141-158.Available at:

Tabuc, C., 2007. Fungal flora of different substrates and optimal conditions for the production of mycotoxins. Doctoral Dissertation.

Tantaoui-Elaraki, A. and B. Le Tutour, 1985. Possible mycotoxin contamination of olives and olive products: Latest developments. Oleagineux, 40(8): 451-454.

Thakare, S.K., R.S. Dewan and K.C. Gulati, 1969. Build-up of DDT in human fat-oils and oilseeds as possible source. Pesticide, 3: 13-14.

Thompson, C. and S.E. Henke, 2000. Effect of climate and type of storage container on aflatoxin production in corn and its associated risks to wildlife species. Journal of Wildlife Diseases, 36(1): 172-179.Available at:

Tian, X., J.-X. Ruan, J.-Q. Huang, C.-Q. Yang, X. Fang, Z.-W. Chen, H. Hong, L.-J. Wang, Y.-B. Mao and S. Lu, 2018. Characterization of gossypol biosynthetic pathway. Proceedings of the National Academy of Sciences, 115(23): E5410-E5418.Available at:

Tomlin, C.D.S., 2003. The pesticide manual: A world compendium. 13th Edn., Alton Hampshire: British Crop Protection Council.

Tozlovanu, M., 2008. Evaluation of the risk of food contamination with nephrotoxic and carcinogenic mycotoxins (notably ochratoxin A): validation of exposure and effect biomarkers. Doctoral Thesis.

Vaitilingom, G., 2007. Extraction, packaging and use of pure fuel vegetable oils. Challenges and perspectives of biofuels for Africa. Available from

Van Duijn, G. and G. Den Dekker, 2010. Unilever food safety assurance system for refined vegetable oils and fats. Oléagineux, Corps gras, Lipides, 17(2): 100-103.Available at:

Vander, J.D.L. and R. Royer, 2000. Gossypol prototype of inhibitors targeted to dinucleotide folds. Current Medicinal Chemistry, 7(4): 479-498.Available at:

Velez, D.L.C.J.F., E. Rachou, M.T. Martelot, B. Ducot, L. Multigner and P.F. Thonneau, 2001. Male infertility risk factors in a French military population. Human Reproduction, 16(3): 481-486.Available at:

Villier, A. and C. Genot, 2006. Physico-chemical and sensory approach to the oxidation of lipids in emulsions. Oleageneux, Corps Gras, Lipides, 13(4): 1-7.

Vine, M.F., L. Stein, K. Weigle, J. Schroeder, D. Degnan, C.-K.J. Tse and L. Backer, 2001. Plasma 1, 1-dichloro-2, 2-bis (p-chlorophenyl) ethylene (DDE) levels and immune response. American Journal of Epidemiology, 153(1): 53-63.Available at:

Vix, H., P. Eaves, H. Gardner and M. Lambou, 1971. Degossypolized cottonseed flour—the liquid cyclone process. Journal of the American Oil Chemists’ Society, 48(10): 611-615.Available at:

Waites, G., C. Wang and P. Griffin, 1998. Gossypol: Reasons for its failure to be accepted as a safe, reversible male antifertility drug. International Journal of Andrology, 21(1): 8-12.Available at:

Waites, G.M.H., 1986. Regulation of male fertility: Recent progress. Bulletin of the World Health Organization, 64(4): 493.

Wang, J., L. Tao, X. Wu, L. Lin, J. Wu, M. Wang and G. Zhang, 1992. Differential binding of (+) and (−) gossypol to plasma protein and their entry into rat testis. Reproduction, 95(1): 277-282.Available at:

Wang, M.-Z., J.-S. Wang, B.-L. Li and F.-Y. GA


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This study received no specific financial support.

Competing Interests:

The authors declare that they have no competing interests.


All authors contributed equally to write manuscript and reviewed. We thank our Laboratory (LaBIA).

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( 24 ) Alleviating Climate Change Effect using Environmentally Friendly Process to Preserve Wood against Biodeteriaorating Agent
( 25 ) A Brief Discussion on Curdling Technology, Microbiological Risks and Contaminants of Fermented Milk Named Biisim Missiga
( 27 ) Effect of Whole Inedible Date and Amino Acid Supplementation on Growth Performance of Ross 308 Broiler Chicks
( 28 ) Edible Films and Coatings: A Good Idea From Past to Future Technology