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International Journal of Veterinary Sciences Research

September 2016, Volume 2, 3, pp 15-21

Biochemical and Electron Microscopic Changes Induced by Giardia in Experimentally Infected Lambs

Reham Samir Elmadawy


Mervat E.I. Radwan


Mohamed Abd El-Aziz Mahmoud

Reham Samir Elmadawy 1
Mervat E.I. Radwan 2

Mohamed Abd El-Aziz Mahmoud 3

  1. Parasitology Department, Faculty of Veterinary Medicine , Benha University, Moshtohor, Toukh, 13736, Egypt 1

  2. Infectious Diseases Department, Veterinary Hospital, Faculty of Veterinary Medicine , Benha University, Moshtohor, Toukh, 13736, Egypt 2

  3. Physiology Department, Faculty of Veterinary Medidine, Assuit University, New valley, Egypt 3

Pages: 15-21

DOI: 10.18488/journal.110/2016.2.3/

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Article History:

Received: 13 October, 2016
Revised: 11 November, 2016
Accepted: 17 November, 2016
Published: 21 November, 2016


The present study was conducted to evaluate the effect of Giardia on the biochemical serum constituents of experimentally infected lambs, in addition to studying the  observed alterations accompanying Giardia infection in the intestinal mucosa using scanning electron microscopy. Twenty lambs were allotted into two equal groups, Group A ( non infected) was kept as control negative and Group B (infected) was orally inoculated by 104 Giardia cysts. The biochemical changes were assessed in both groups on 7th. 14th, 21th and 30th days post inoculation (dpi). The study disclosed that Giardia induced a significant drop in the levels of serum electrolytes (chloride,sodium, potassium and calcium), blood glucose, different enzymes (lipase, amylase and  alkaline phosphatase).  Furthermore, the levels of urea, liver enzymes (alanine aminotransferase and aspartate aminotransferase), inflammatory marker (C-reactive protein) and oxidative stress marker (malondialdehyde) were  elevated, but nitric oxide was declined  by 30th dpi. The scanning electron microscopy of the intestinal mucosa of the infected lambs revealed a notable alteration which was fully explained. All the presented results interpret the pathophysiological effect of Giardia which adversely affects the health status of lambs.
Contribution/ Originality


Giardia, Biochemical parameters, Oxidative stress, Electron microscopy.



  1. C. Ragbetli, P. Tanritanir, H. Yilmaz, I. Yoruk, O. H. Diskapi, and Y. Beyazit "Biochimical parameters in naturaly infected calves with giardiasis after treatment with Albendazol Van Province, Turky," Indian Journal of Animal Research, vol. 48, pp. 38-44, 2014.
  2. A. C. De Santis-Kerr, M. Raghavan, N. W. Glickman, R. J. Caldanaro, G. E. Moore, H. B. Lewis, P. M. Schantz, and L. T. Glickman, "Prevalence and risk factors for Giardia and coccidia species of pet cats in 2003-2004," Journal of Feline Medicine & Surgery, vol. 8, pp. 292-301, 2006.
  3. I. S. Hamnes, B. Gjerde, and L. Robertson, "Prevalance of Giardia and cryptosporidium in dairy calves in three areas of Norway," Veterinary Parasitology, vol. 140, pp. 204-216, 2006.
  4. A. Gul, M. Cicek, and O. Kilinc, "Prevalence of Eimeria spp., Cryptosporidium spp. and Giardia spp. in Calves in the Van Province," Acta Parasitologica Turcica, vol. 32, pp. 202–204, 2008.
  5. F. Aloisio, G. Filippini, P. Antenucci, E. Lepri, G. Pezzotti, M. Caccio, and E. Pozio, "Severe weight loss in lambs infected with Giardia duodenalis assemblage B," Veterinary Parasitology, vol. 142, pp. 154–158, 2006.
  6. A. G. Buret, "Mechanisms of epithelial dysfunction in giardiosis," Gut, vol. 56, pp. 328–335, 2007.
  7. A. G. Buret, "Pathophysiology of enteric infections with Giardia duodenalius," Parasite, vol. 15, pp. 261-265, 2008.
  8. M. Tuck, W. D. Chan, D. Chia, K. A. Godwin, E. W. Grizzle, E. K. Krueger, W. Rom, M. Sanda, L. Sorbara, S. Stass , W. Wang, and E. D. Brenner, "Standard operating procedures for serum and plasma collection: Early detection research network consensus statement standard operating procedure integration working group," Journal of Proteome Research, vol. 8, pp. 113–117, 2009.
  9. K. W. Miller and C. S. Yang, "An isocratic high-performance liquid chromatography method for the simultaneous analysis of plasma retinol, a-tocopherol and various carotenoids," Analytical Biochemistry, vol. 145, pp. 21- 26, 1985.
  10. B. J. Zaspel and S. Csallany, "Determination of alpha-tocopherol in tissues and plasma by high-performance liquid chromatography," Analytical Biochemistry, vol. 130, pp. 146-150, 1983.
  11. S. L. Reynolds and H. J. Judd, "Rapid procedure for the determination of vitamins A and D in fortified skimmed milk powder using high-performance liquid chromatography," Analyst, vol. 109, pp. 489-492, 1984.
  12. J. Kuo, Electron microscopy, methods and protocols, 2nd ed. New Jersey: Humana Press Inc, 2007.
  13. R. Steel, J. Torrie, and D. Dickey, Principles and procedures of statistics: A biometrical approach, 3rd ed. New York: McGraw-Hill, 1997.
  14. M. Krajnicakova, N. S. Kovae, M. Kostecky, I. Valocky, I. Maraeek, I. Sutiakova, and L. Lenhardt, "Selected clinico-biochemical parameters in the puerperal period of goats," Bull. Vet. Re.s Inst. Pulawy, vol. 47, pp. 177-182, 2003.
  15. R. Kulcu and F. Yur, "A study of some serum mineral levels before and during pregnancy and during lactation period of sheep and cattle," Biological Trace Element Research, vol. 92, pp. 275-279, 2003.
  16. A. Buret, J. A. Hardin, M. E. Olson, and D. G. Gall, "Pathophysiology of small intestinal malabsorption in gerbils infected with Giardia lamblia," Gastroenterology, vol. 103, pp. 506–513, 1992.
  17. A. Cevallos, S. Carnaby, M. James, and J. G. Farthing, "Small intestinal injury in a neonatal rat model of Giardiasis giardiasis is strain dependent," Gastroenterology, vol. 109, pp. 766–773, 1995.
  18. S. Ayaz, F. U. Khan, I. Hussain, R. U. Ihsan, and Z. Muhammad, "Efficacy of metranidazole and albendazole against giardiosis and its effects on body weight and feed intake in cattle," World Applied Biosciences Journal, vol. 10, pp. 421-425, 2010.
  19. L. S. Resta, T. D. Langford, F. D. Gillin, and E. K. Barrett, "Altered chlorid secretory responses in HT29/Cl. 19A cells infected with Giardia lamblia," Gastroenterology, vol. 18, p. A684, 2000.
  20. S. Gorowara, N. K. Ganguly, and R. C. Mahajan, "Study on the mechanism of Giardia lamblia-induced diarrhea in mice," Biochem Biophys Acta, vol. 1138, pp. 122-126, 1992.
  21. F. Barkas, A. E. Kei, and M. Elisaf, "Electrolyte and acid-base disorders in inflammatory bowel disease," Annals of Gastroenterology, vol. 26, pp. 23–28, 2013.
  22. L. A. G. Rosa, M. A. Gomes, A. V. Mundim, M. J. S. Mundim, E. L. Pozzer, E. S. M. Faria, J. C. Viana, and M. C. Cury, "Infection of dogs by experimental inoculation with human isolates of Giardia duodenalis: Clinical and laboratory manifestations," Veterinary Parasitology, vol. 145, pp. 37-44, 2007.
  23. G. P. Brash, F. B. Culen, K. R. Stoelting, K. M. Cahalan, and M. Stock, Clinical Anesthesia, 6th ed. Hardcover: Lippincott Williams and Wilkins, 2009.
  24. F. Seow, P. Kateralis, and M. Ngu, "The effect of Giardia lambia trophozoites on trypsin, chymotrypsin and amylase in vitro," Parasitology, vol. 106, pp. 233-238, 1993.
  25. S. Nisar, F. M. Khan, and A. M. Azmi, "Effect of parasitic infection on glucose and lipase of host pigeon (Columba livia) tissues," Journal of Basic & Applied Sciences, vol. 8, pp. 478-481, 2012.
  26. E. M. Macy, E. H. Timothy, and P. T. Russell, "Variability in the measurement of C-reactive protein in healthy subjects: Implications for reference intervals and epidemiological applications," Clinical Chemistry, vol. 43, pp. 52-58, 1997.
  27. S. Safi, "Acute phase proteins - analysis, clinical applications and potentials, inflammatory diseases - immunopathology, clinical and pharmacological bases, Dr Mahin Khatami (Ed.), In tech." Retrieved from:, 2012.
  28. E. Kılıç, S. Yazar, R. Saraymen, and H. Özbilge, "Serum malondialdehyde level in patients infected with Ascaris lumbricoides," World Journal of Gastroenterology, vol. 9, pp. 2332-2334, 2003.
  29. S. Şimşek, A. Yüce, and E. A. Ütük, "Determination of serum malondialdehyde levels in sheep naturally infected with dicrocoelium dendriticum," F.Ü. Sağlık Bil. Dergisi, vol. 20, pp. 217-220, 2006.
  30. P. D. Fernandes and J. Assreuy, "Role of nitric oxide and superoxide in Giardia lamblia killing Brazilian," Journal of Medical and Biological Research, vol. 30, pp. 93-99, 1997.
  31. M. R. Bayraktar, N. Mehmet, and R. Durmaz, "Serum cytokine changes in Turkish children infected with Giardia lamblia with and without allergy: Effect of metronidazole treatment," Acta Tropica, vol. 95, pp. 116-122, 2005.
  32. H. Nahrevanian, "Involvement of nitric oxide and its up/down stream molecules in the immunity against parasitic infections," Brazilian Journal of Infectious Diseases, vol. 13, pp. 440-448, 2009.
  33. [M. Imran, I. Ahmad, and S. M. Malik, "Prevalence of Giardia lamblia and gastrointestinal parasites in ruminants," Global Veterinaria, vol. 11, pp. 708-713, 2013.
  34. R. Khanna, K. Joshi, K. Kum, A. K. Malik, and V. K. Vinayak, "An ultrastructural analysis of changes in surface architecture of intestinal mucosa following Giardia lamblia infection in mice," Gastroenterologia Japonica, vol. 25, pp. 649-658, 1990.
  35. K. G. Scott, M. R. Logan, G. M. Klammer, D. A. Teoh, and A. G. Buret, "Jejunal brush border microvillous alterations in Giardia muris-infected mice: Role of T lymphocytes and interleukin-6," Infection and Immunity, vol. 68, pp. 3412–3418, 2000.
  36. K. G. Scott, L. Yu, and A. G. Buret, "Role of CD8+ and CD4+ T lymphocytes in jejunal mucosal injury during murine giardiasis," Infection and Immunity, vol. 72, pp. 3536-3542, 2004.


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This study received no specific financial support.

Competing Interests:

The authors declare that they have no competing interests.


All authors contributed equally to the conception and design of the study.

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