DNA templated metal nanoparticles have attracted a lot of interest and have been widely applied in biomedical fields. Silver, platinum, palladium and copper nanostructure templated by DNA have been widely reported. While the synthesis of DNA templated gold nanoparticles (Au NPs) was limited. In this article, we presented a novel one-step method for synthesizing Au NPs stabilized by oligonucleotides. UV absorption spectroscopy showed that the formation of Au NPs was associated with pH value, DNA sequence, and DNA concentration. Under neutral pH condition, the UV absorption spectra of Au NPs showed that the peak width was narrow. The blue-shift (from about 540 to 524 nm) of surface plasmon resonance (SPR) peak occurred when Au NPs formed under higher pH (9.6) condition comparing with neutral pH condition. Slight red-shift of the SPR peak occurred with increase in concentration of oligonucleotides. For C10 oligonucleotide stabilized Au NPs, the peak width of UV absorption spectra was wider than A10 and T10 oligonucleotide stabilized Au NPs, especially under lower pH value and lower oligonucleotide concentration. The wider peak width and red-shift were due to aggregation of Au NPs which can be proved by SEM image.