In this study, the mutagenicity and genotoxicity of urban wastewater of the city of Guelma in Algeria were examined between April 2012 and April 2013. For this, two biological tests, namely Ames and chromosomal aberrations (CA) test in Allium cepa root tips were employed on the samples collected from five different sampling stages (S1–S5). In Ames test, two strains of Salmonella typhimurium TA98 and TA100 with or without metabolic activation (S9-mix) were used. All water samples were found to be mutagenic to S. typhimurium TA98 with or without S9-mix. A significant decrease in mitotic index (MI) was observed with a decrease in the percentage of cells in the prophase.
Keywords:
Mutagenicity, Genotoxicity, Urban wastewater, Ames test, Allium test
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