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Journal of Diseases

March 2019, Volume 6, 1, pp 1-6

Staining Effect of Methanolic Extract of Hibiscus Sabdariffa Calyx on Thin Peripheral Blood Smear

Emmanuel Asuquo Etim


Ezekiel Friyanda Chagwa


Emmanuel Ifeanyi Obeagu


Akpan Akaninyene Udokang

Emmanuel Asuquo Etim 1 ,

Ezekiel Friyanda Chagwa 1 Emmanuel Ifeanyi Obeagu 3
Akpan Akaninyene Udokang 1 
  1. Laboratory Department, Federal Medical Centre Yola, Adamawa State, Nigeria 1

  2. Department of University Health Services, Micheal Okpara University of Agriculture, Nigeria 3

Pages: 1-6

DOI: 10.18488/journal.99.2019.61.1.6

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Article History:

Received: 09 October, 2018
Revised: 14 November, 2018
Accepted: 19 December, 2018
Published: 04 February, 2019


Hibiscus sabdariffa is a species of Hibiscus in the Malvaceae family. Hibiscus sabdariffa is widely consumed as Zobo drink beverage in Nigeria with many health benefits, and it is believed that the brilliant reddish coloration of methanolic extract of this plant’s calyx may have a staining effect on thin blood smear. Hence, this study aims to examine the staining effect of methanolic extract of Hibiscus sabdariffa calyx on thin peripheral blood films in other to obtain more surrogates for hematological stains. Dried calyx of Hibiscus sabdariffa was obtained commercially from Yola. The calyces were pulverized to fine powder and 10g of the powder was soaked in 50ml of absolute methanol for 12hours to obtain methanolic extract. PH of the extract was determined using PH meter. The methanolic extract was used to stain thin peripheral blood smear. Results: Raw methanolic extract of Hibiscus sabdariffa calyx had pH of 1. The extract produces poor staining effect and impacted a pale pinkish-orange appearance on the red blood cells with pseudo large central pallor microscopically. While field stain and Leishman stain produces a dark pink coloration with normal pallor on the red cell. Raw methanolic extract of Hibiscus sabdariffa calyx was acidic and it impacted a poor pale pinkish-orange coloration with pseudo large pallor on red blood cell in thin peripheral blood smear. More research to get a molar equilibrium of acidic and basic substances in this plant’s calyx extract is recommended in other to obtain more cost effective surrogates for good hematological stains.
Contribution/ Originality
This study contributes in the existing literature of the medical importance of Hibiscus sabdariffa calyx with emphasis on the relevance of this extract in medical laboratory practice.


Thin blood smear, Hibiscus sabdariffa calyx.




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This study received no specific financial support.

Competing Interests:

The authors declare that they have no competing interests.


All authors contributed equally to the conception and design of the study.

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