Contact Us

For Marketing, Sales and Subscriptions Inquiries
2637 E Atlantic Blvd #43110
Pompano Beach, FL 33062

Conference List

Journal of Diseases

March 2018, Volume 5, 1, pp 24-32

Characterization and Antimicrobial Resistance Profile of Pseudomonas Aeruginosa Isolated from Clinical and Environmental Samples

Doughari, J.H.


Ahmad, A.A.


Nafisat K. M.

Doughari, J.H. 1 ,

Ahmad, A.A. 1 Nafisat K. M. 1 
  1. Department of Microbiology, School of Pure and Applied Sciences, Modibbo Adama University of Technology, Yola, Nigeria 1

Pages: 24-32

DOI: 10.18488/journal.99.2018.51.20.28

Share :

Article History:

Received: 09 July, 2018
Revised: 31 August, 2018
Accepted: 03 September, 2018
Published: 05 September, 2018


Pseudomonas aeruginosa has emerged as a potential problematic Gram negative pathogen. The present study investigated the distribution of P. aeruginosa from the various clinical (wound and post-surgical wound) and environmental (water, industrial effluents and air) samples. The antibiotic resistance test was performed using the disc diffusion method according to NCCLS guidelines. Multidrug resistant genes among the isolates were characterized using PCR. 18 of the isolates with multidrug resistance showed least resistance to ceftazidime (23 %), gentamicin (20 %) and ciprofloxacin (31 %) from clinical isolates and 60, 38.5 and 60 % respectively from environmental isolates. Over 78 % of the isolates were polyvalent 1 strains, 17 % were polyvalent 2 and 5 % were polyvalent 3 strains. Polyvalent 1 were mostly strains from clinical samples while polyvalent 2 and 3 were strains from both (clinical and environmental) samples investigated. PCR analysis of multidrug resistant P. aeruginosa indicates that, PA-SS species specific signature sequences were present in all (100%) the isolates investigated. There was high emergence of antibiotic resistance in the environment and hospital investigated. Proper monitoring, regulating indiscriminate use of chemicals in the environment and restricting the use of antibiotics without susceptibility tests or prescription by a qualified physician should be adopted.
Contribution/ Originality
This study documents the prevalence of drug resistant strains of P. aeruginosa isolated from clinical and environmental sources in Yola, Adamawa State., Nigeria.


Pseudomonas aeruginosa, Multidrug-resistance, Signature sequences, Genes, Clinical samples, Polymerase chain reaction, Environmental isolates, Polyvalent strain, Prescription.



Google Scholor ideas Microsoft Academic Search bing Google Scholor


The study was funded by the Year 2012-2014 (Merged) TETFUND Research Projects (RP) Intervention Grant (5th Batch) of the Federal Government of Nigeria under the University Research Unit of the Modibbo Adama University of Technology, Yola.

Competing Interests:

The authors declare that they have no competing interests.


All authors contributed equally to the conception and design of the study.

Related Article

( 1 ) Characterization and Antimicrobial Resistance Profile of Pseudomonas Aeruginosa Isolated from Clinical and Environmental Samples
( 2 ) Screening of Antimicrobial Activity of Essential Oil of Thymus Vulgarison Some Pathogens
( 4 ) Effect of Different Chronotype on Comorbidity and Resistance to Psycho-Emotional Stress in Hypertension Associated With Hepato-Biliary Pathology in the North
( 5 ) Impaired Immune Phenotype of Circulating Endothelial-Derived Microparticles in None-Diabetic Patients with Chronic Heart Failure: Impact on Insulin Resistance
( 7 ) Effect of Dietary Supplementation with Cyphostemma Digitatum on Serum Lipid Profile and Liver Enzymes in Hyperlipidemic Subjects
( 8 ) Serum Lipid Profile and Leptin Levels in Asthmatic Libyan Children
( 9 ) Profile of Physiotherapist Educators in Anglophone West African Countries: A Cross-Sectional Study
( 10 ) Profile of Patients Visiting a Tertiary Eye Hospital and their Perception Regarding Barrier for Eye Care Services in the Western Saudi Arabia
( 12 ) Gender Based Differences in Epidemiologic and Clinical Profile of Adult Vitiligo
( 13 ) Mortalities Profile among Dubai Population 2017, Utilizing Breakdown Fractions for Intervention and Forecasting of Future Epidemiologic Transition - Dubai, UAE 2019
( 17 ) Retrospective Study on Outcome of In-Patient Treatment of Severe Acute Malnutrition in Jimma University Specialized Hospital from September 2011-September 2012
( 18 ) How Do Patients Evaluate the New Health Care System in General Practice? Results from the European Project on Patient Evaluation of General Practice Care (Europep) in Adana, Turkey
( 19 ) Lifetime Cancer Risk of Gamma Radioactivity Results from Smoking
( 20 ) The Risk of Ovarian Cancer in Women Fed with Milk Obtained from Goats Having Different Birth Type and Kid Sex
( 21 ) Determination of the Seasonality of Bovine Tuberculosis Disease from the Risk Based Disease Management
( 23 ) Development and Quality Evaluation of Complementary Food Formulated from Sorghum, Soybean and Sesame Flour Blends
( 24 ) Ultrasound Guided Axillary Node Sampling in Patients of Carcinoma Breast with Clinically Negative Axilla: A Pilot Study