2637 E Atlantic Blvd #43110
Pompano Beach, FL 33062
50th International Scientific Conference on Sport, Medical and Health Sciences Vennue: Hotel Grand Flora Dubai, UAE Al- Rigga Road Deira , Near Al-Rigga Metro station , Deira Dubai UAE Date: 20-Dec-2018 More Details
7th International Academic Conference on Medical and Health Sciences Vennue: Hotel Istana Kuala Lumpur City Centre 73 Jalan Raja Chulan, Kuala Lumpur, 50200, Malaysia Date: 29-Dec-2018 More Details
Oocyte quality is one of the main factors for the success of in vitro fertilization protocols. Apoptosis is known to affect oocyte quality and may impair subsequent embryonic development and implantation. The aim of this study was to investigate the apoptosis rate of single and pooled cumulus cells of cumulus cell–oocyte complexes (COCs), as markers of oocyte quality, prior to intracytoplasmatic sperm injection (ICSI).We investigated the apoptosis rate by TUNEL assay (DNA fragmentation) and caspase-3 immunoassay of single and pooled cumulus cells of COCs. The results showed that DNA fragmentation in cumulus cells was remarkably lower in patients who achieved a pregnancy than in those who did not. Cumulus cell apoptosis rate could be a marker for the selection of the best oocytes to be fertilized by intracytoplasmatic sperm injection.
This study is one of very few studies which have investigated the apoptosis rate by TUNEL assay and caspase-3 immunoassay of single and pooled human cumulus cells of COCs. The apoptosis rate could be a marker for the selection of the best oocytes to be fertilized by intracytoplasmatic sperm injection.
L. L. Veeck, "Oocyte assessment and biological performance," Ann. N Y Acad. Sci., vol. 541, pp. 259-274, 1988.
P. A. Almeida and V. N. Bolton, "The relationship between chromosomal abnormalities in the human oocyte and fertilization in vitro," Hum. Reprod., vol. 9, pp. 343-346, 1994.
P. A. Almeida and V. N. Bolton, "Immaturity and chromosomal abnormalities in oocytes that fail to develop pronuclei following insemination in vitro," Hum. Reprod., vol. 3, pp. 8229-232, 1993.
R. R. Angell, J. Xian, and J. Keith, "Chromosome anomalies in human oocytes in relation to age," Hum. Reprod., vol. 8, pp. 1047-1054, 1993.
C. W. J. D. Kok, S. M. Looman, and E. R. Velde, "High number of oocytes obtained after ovarian hyperstimulation for in vitro fertilization or intracytoplasmic sperm injection is not associated with decreased pregnancy outcome," Fertil Steril, vol. 85, pp. 918–924, 2006.
L. Zhang, S. Jiang, P. J. Wozniak, X. Yang, and R. A. Godke, "Cumulus cell function during bovine oocyte maturation, fertilization, and embryo development in vitro," Mol. Reprod Dev., vol. 40, pp. 338-344, 1995.
L. Gregory and H. J. Leese, "Determinants of oocyte and preimplantation embryo quality: Metabolic requirements and potential role of cumulus cells," J. Br. Fertil Soc. (Human Reprod Suppl), vol. 11, pp. 96-102, 1996.
T. Mori, T. Amano, and H. Shimizu, "Roles of gap junctional communication of cumulus cells in cytoplasmic maturation of porcine oocytes cultured in vitro," Biol. Reprod, vol. 62, pp. 913–919, 2000.
G. Ruvolo, L. Bosco, A. Pane, G. Morici, E. Cittadini, and M. C. Roccheri, "Lower apoptosis rate in human cumulus cells after administration of recombinant luteinizing hormone to women undergoing ovarian stimulation for in vitro fertilization procedures," Fertil Steril, vol. 87, pp. 542-546, 2007.
E. Warzych, E. Pers-Kamczyc, A. Krzywak, S. Dudzi?ska, and D. Lechniak, "Apoptotic index within cumulus cells is a questionable marker of meiotic competence of bovine oocytes matured in vitro," Reprod Biol., vol. 13, pp. 82-87, 2013.
Y. Q. Yuan, A. Van Soom, J. L. M. R. Leroy, J. Dewulf, A. Van Zeveren, A. De Kruif, and L. J. Peelman, "Apoptosis in cumulus cells, but not in oocytes, may influence bovine embryonic developmental competence," Theriogenology, vol. 63, pp. 2147–2163, 2005.
G. Ruvolo, R. R. Fattouh, L. Bosco, A. M. Brucculeri, and E. Cittadini, "New molecular markers for the evaluation of gamete quality," J. Assist. Reprod Genet., vol. 30, pp. 207-212, 2013.
M. Mattioli and B. Barboni, "Signal transduction mechanism for LH in the cumulus-oocyte complex," Mol. Cell Endocrinol, vol. 161, pp. 19-23, 2000.
V. N. Bolton, S. M. Hawes, C. T. Taylor, and J. H. Parsons, "Development of spare human preimplantation embryos in vitro: An analysis of the correlations among gross morphology, cleavage rates, and development to the blastocyst," J. In Vitro Fert. Embryo. Transf., vol. 6, pp. 30-35, 1989.
K. Nakahara, H. Saito, T. Saito, M. Ito, N. Ohta, T. Takahashi, and M. Hiroi, "The incidence of apoptotic bodies in membrana granulosa can predict prognosis of ova from patients participating in in vitro fertilization programs," Fertil Steril, vol. 68, pp. 312-317, 1997.
K. S. Lee, B. S. Joo, Y. J. Na, M. S. Yoon, O. H. Choi, and W. W. Kim, "Cumulus cells apoptosis as an indicator to predict the quality of oocytes and the outcome of IVF-ET," J. Assist Reprod Genet., vol. 18, pp. 490-498, 2001.
R. B. Staigmiller and R. M. Moor, "Effect of follicle cells on the maturation and developmental competence of ovine oocytes matured outside the follicle," Gamete Res., vol. 9, pp. 221–229, 1984.
M. A. El-Fouly, B. Cook, M. Nekola, and A. V. Nalbandov, "Role of the ovum in follicular luteinization," Endocrinology, vol. 87, pp. 286-293, 1970.
R. Li, R. J. Norman, D. T. Armstrong, and R. B. Gilchrist, "Oocyte-secreted factor(S) determine functional differences between bovine mural granulosa cells and cumulus cells," Biol Reprod, vol. 63, pp. 839-845, 2000.
L. Bosco, G. Ruvolo, G. Morici, M. Manno, E. Cittadini, and M. C. Roccheri, "Apoptosis in human unfertilized oocytes after intracytoplasmic sperm injection," Fertil Steril, vol. 84, pp. 1417-1423, 2005.
E. Høst, A. L. Mikkelsen, S. Lindenberg, and S. Smidt-Jensen, "Apoptosis in human cumulus cells in relation to maturation stage and cleavage of the corresponding oocyte," Acta Obstet Gynecol Scand, vol. 79, pp. 936-940, 2000.
O. Moffatt, S. Drury, M. Tomlinson, M. Afnan, and D. Sakkas, "The apoptotic profile of human cumulus cells changes with patient age and after exposure to sperm but not in relation to oocyte maturity," Fertil Steril, vol. 77, pp. 1006-1011, 2002.
E. Høst, A. Gabrielsen, S. Lindenberg, and S. Smidt-Jensen, "Apoptosis in human cumulus cells in relation to zona pellucida thickness variation, maturation stage, and cleavage of the corresponding oocyte after intracytoplasmic sperm injection," Fertil Steril, vol. 77, pp. 511-515, 2002.
M. Toya, H. Saito, N. O. T. Saito, T. Kaneko, and M. Hiroi, "Oocyte quality in patients with moderate and severe endometriosis," Fertil Steril, vol. 73, pp. 344-350, 1999.
P. Falcone, L. Gambera, M. Pisoni, V. Lofiego, V. De Leo, L. Mencaglia, and P. Piomboni, "Correlation between oocyte preincubation time and pregnancy rate after intracytoplasmic sperm injection," Gynecol Endocrinol, vol. 24, pp. 295-299, 2008.