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50th International Scientific Conference on Sport, Medical and Health Sciences
Vennue: Hotel Grand Flora Dubai, UAE Al- Rigga Road Deira , Near Al-Rigga Metro station , Deira Dubai UAE
Date: 20-Dec-2018
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7th International Academic Conference on Medical and Health Sciences
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Date: 29-Dec-2018
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Protective Effect of L-Ascorbic Acid (Vitamin C) On Mercury Detoxication and Physiological Aspects of Albino Rats

Somaya M.Ismail, Hayat A.Ismail, Ghidaa M. Al-Sharif

Somaya M.Ismail 1 ,

Hayat A.Ismail 1 Ghidaa M. Al-Sharif 3

  1. Cairo University – Faculty of Science, Department of Zoology, Giza, Egypt 1

  2. Biology Department, Faculty of Science, King Abd El Aziz University, Giza, Egypt 3


The present study was carried out to investigate the protective effect of  L-ascorbic acid (A.A.), against  mercury toxicity in albino rats. Twenty four males were divided into three groups. The control was fed a basic diet, whereas the other two groups were treated either by Hg alone (1g HgCl2/Kg food) or Hg-ascorbic acid (1g HgCl2/Kg food + 5% ascorbic acid), respectively, for 35 consecutive days. The following serum parameters were estimated; alkaline phosphatase (ALP), aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT), urea, creatinine, uric acid, triglicerides, total cholesterol, HDL-cholesterol and LDL cholesterol.
The present data obtained found that a significant decrease in serum ALP activity in mercury treated group, accompanied by a significant increase in serum ASAT. Furthermore, serum urea concentration was significantly higher in the mercury group compared to the control. However, the concentration of urea and the activity of ALP and ASAT of the Hg+ascorbic acid group were not statistically different from the control. Moreover, no significant variations were recorded concerning ASAT, creatinine and uric acid. The lipid profile; triglycerides, total cholesterol, HDL-cholesterol and LDL-cholesterol were not significantly varied between the three groups, despite the observed elevated concentration of triglycerides in Hg+ascorbic acid group. In conclusion, ascorbic acid may partially help in the protection against mercury intoxication and it could also be considered a safe nutritional source.

Contribution/ Originality
This study is one of very few studies which have investigated protective effect of l-ascorbic acid mercury detoxication and physiological aspects and The paper’s primary contribution is finding that ascorbic acid may partially help in the protection against mercury intoxication and it could also be considered a safe nutritional source.




  1. J. Fu, H. Wang, S. M. Billah, H. Yu, and X. Zhang, "Heavy metals in seawater, sediments and biota from the coastal area of Yancheng city, China," Environ Toxicol Chem., vol. 12, pp. 1002-1010, 2014.
  2. B. O. Stephan, M. Kathleen, and S. D. Carty, "Mercury exposure and children's health," Current Problems in Pediatric and Adolescent Health Care, vol. 40, pp. 186-215, 2010.
  3. S. J. Flora, M. Mittal, and A. Mehta, "Heavy metal induced oxidative stress & its possible reversal by chelation therapy," Indian J. Med. Res., vol. 128, pp. 501-23, 2008.
  4. C. Abdennour, K. Khellili, M. Boulakoud, A. Nazzal, S. Boubsil, and S. Slimani, "Urinary markers of workers chronically exposed to mercury vapour," Environ. Res. Section A., vol. 89, pp. 245-249, 2002.
  5. C. Ma, D. Xie, L. Huang, L. Sun, Q. Xu, G. Li, and L. Hao, "Protective effect of salicylic acid on Hg(0) intoxication in mice," Inhal. Toxicol., vol. 25, pp. 398-404, 2013.
  6. V. R. Funk, M. S. Rotta, D. Bohrer, R. L. Puntel, M. Farina, M. Aschner, and J. B. Rocha, "Diphenyl diselenide,a simple organoselenium compound ,decreases methyl mercury –induced cerebral ,hepatic and renal oxidative stress and mercury deposition in adult mice," Brain .Res. Bull., vol. 79, pp. 77-84, 2009.
  7. E. Moreira, J. De Oliveira, M. F. Dutra, D. B. Santos, C. A. Gonçalves, E. M. Goldfeder, A. F. de Bem, R. D. Prediger, M. Aschner, and M. Farina, "Does methylmercury-induced hypercholesterolemia play a causal role in its neurotoxicity and cardiovascular disease?," Toxicol Sci., vol. 130, pp. 373-382, 2012.
  8. S. Hussain, A. Atkinson, S. J. Thompson, and A. T. Khan, "Accumulation of mercury and its effect on antioxidant enzymes in brain, liver, and kidneys of mice," J. Environ. Sci Health B., vol. 34, pp. 645-660, 1999.
  9. M. Mahboob, K. F. Shireen, A. Atkinson, and A. T. Khan, "Lipid peroxidation and antioxidant enzyme activity in different organs of mice exposed to low level of mercury," J. Environ. Sci. Health B., vol. 36, pp. 687-697, 2001.
  10. A. L. Ganser and D. A. Kirschner, "The interaction of mercurials with myelin: Comparison of in vitro and in vivo effects," Neurotoxicol., vol. 6, pp. 63-78, 1985.
  11. I. Cloez, O. Dumont, M. Piciotti, and J. M. Bourre, "Alterations of lipid synthesis in the normal and dysmyelinating trembler mouse sciatic nerve by heavy metals (Hg, Pb, Mn, Cu, Ni)," Toxicol., vol. 46, pp. 65-71, 1987.
  12. B. M. Tolbert, "Ascorbic acid metabolism and physiological function," Int. J. Vit. Nutr. Res., vol. 19, pp. 127-142, 1979.
  13. L. R. McDowell, Vitamins in animal nutrition. San Diego, Brazil: Academic Press, 1989.
  14. C. Lim, "Nutrition and feeding of tilapias," Fish Disease and Parasite Research, vol. 4, pp. 95-107, 1996.
  15. O. Navarre and J. E. Halver, "Disease resistance and humoral antibody production in rainbow trout fed high levels of vitamin C," Aquaculture, vol. 79, pp. 207-221, 1989.
  16. N. K. Agrawal, C. J. Juneja, and C. L. Mahajan, "Protective role of ascorbic acid in fishes exposed to organochlorine pollution," Toxicol., vol. 11, pp. 369-375, 1978.
  17. L. C. Bapu, P. P. Prakash, and M. Nivsarkar, "Organelle specific enzyme markers as indicators of methylmercury neurotoxicity and antidotal efficacy in mice," Bio Metals, vol. 16, pp. 279–284, 2003.
  18. R. D. Wright and A. Baccavell, "Metals and neurotoxicology," J.Nutr., vol. 173, pp. 2809-2813, 2007.
  19. M. Farina, J. Rocha, and M. Aschner, "Mechanisms of methylmercury-induced neurotoxicity: Evidence from experimental studies," Life Sci., vol. 89, pp. 555-563, 2011.
  20. M. Karapehlivan, M. Ogun, I. Kaya, H. Ozen, H. Deveci, and M. Karaman, "Protective effect of omega-3 fatty acid against mercury chloride intoxication in mice," J .Trace Elem. Med. Biol., vol. 28, pp. 94-9, 2014.
  21. S. Janakat and H. Al-Merie, "Evaluation of hepatoprotective effect of pistacia lentiscus, phillyrealatifolia and nicotiana glauca," J. Ethnopharmacol., vol. 83, pp. 135-138, 2002.
  22. T. Endo, Y. Hotta, K. Haraguchi, and M. Sakata, "Distribution and toxicity of mercury in rats after oral administration of mercury-contaminated whole red meat marketed for human consumption," Chemosphere, vol. 61, pp. 1069-1073, 2005.
  23. M. N. WHO, "International programme on chemical safety," Eenviron Health Criteria, vol. 118, pp. 22-33, 1991.
  24. R. Randao, F. W. Santos, M. Farina, G. Zeni1, D. Bohrer, J. T. Rocha1, and C. W. Nogueira, "Antioxidants and metallothionein levels in mercury-treated mice," Cell Biol. Toxicol., vol. 22, pp. 429–438, 2006.
  25. I. Brake, "Immune status role of vitamins," Feed Mix, vol. 5, pp. 21-24, 1997.
  26. A. K. Soliman, K. Jauncey, and R. S. Roberts, "Water soluble vitamin requirements of tilapia: Ascorbic acid (Vitamin C) requirement of nile tilapia; oreochromis niloticus (L)," Aquaculture and Fisheries Management, vol. 25, pp. 269-278, 1994.
  27. M. Dhawan, D. M. Kachru, and S. K. Tandon, "Influence of thiamine and ascorbic acid supplementation on the antidotal efficacy of thiol chelators in experimental lead toxicity," Arch. Toxicol., vol. 62, pp. 301-304, 1988.
  28. K. S. Ghazaly, "Efficancy of ascorbic acid (Vitamin C) on experimental copper intoxication in Tilapia zillii," Bull. Nat. Inst. Oceanogr. Fish. Egypt., vol. 20, pp. 249-257, 1994.
  29. M. D. Gross, Lipids, oxidation, and cardiovascular disease. Atherosclerosis and oxidant stress. Book Chapter. Ed Springer US.599. Maarouf Tounes: Cherif Abdennour and Nadjet Houaine, 2008.
  30. J. Liu, "Pharmacology of oleanolic acid and ursolic acid," J. Ethnopharmac, vol. 49, pp. 57–68, 1995.
  31. J. L. Rios, M. C. Recio, S. Manez, and R. M. Giner, Natural triterpenoids as anti-inflammatory agents. In: Atta-Ur-Rahman (Ed.).Studies in natural products chemistry: Bioactive natural products vol. 22. Amsterdam: Elsevier,Part C, 2000.
  32. P. L. Goering, D. L. Morgan, and S. F. Ali, "Effects of mercury vapor inhalation on reactive oxygen species and antioxidant enzymes in rat brain and kidney," J. Appl. Toxicol., vol. 22, pp. 167-172, 2002.
  33. P. P. Sood, C. Bapu, N. Sinha, and A. P. Rao, "Cholesterol and triglycerides fluctuations in mice tissues during methylmercury intoxication and monothiols and vitamins therapy," J. Nutr. Environ. Med., vol. 7, pp. 155–162, 1997.
  34. M. A. Huq, M. A. Awal, M. A. Mostofa, and A. R. Ghosh, "Effects of vitamin E and vitamin C on mercury induced toxicity in mice," Progressive Agriculture, vol. 19, pp. 93–100, 2008.
  35. J. C. Eya, "Broken-skull disease in African catfish; clarias gariepinus is related to a dietary deficiency of ascorbic acid," J. World Aquacult. Soc., vol. 27, pp. 493-498, 1996.
  36. J. M. Leonardo, L. Vargas, R. P. Ribeiro, F. Cavichiolo, and H. L. Marques, "Effect of different levels of vitamin C on ectoparasite occurrence, survival rate and total biomass of Thailandese Nile tilapia (Oreochromis Niloticus) larvae. Tilapia aquaculture in the 21st century," in Proceedings from the 5th International Symposium on Tilapia Aquaculture. 3-7 Sept. 2000, Rio de Janeiro, Brazil, 2000.


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