The International Journal of Biotechnology

Published by: Conscientia Beam
Online ISSN: 2306-6148
Print ISSN: 2306-9864
Quick Submission    Login/Submit/Track

Recent Articles

In Vitro Antimicrobial Activities of Crude Extracts of Two Traditionally Used Ethiopian Medicinal Plants against Some Bacterial and Fungal Test Pathogens

Pages: 104-114
Find References

Finding References


In Vitro Antimicrobial Activities of Crude Extracts of Two Traditionally Used Ethiopian Medicinal Plants against Some Bacterial and Fungal Test Pathogens

Search :
Google Scholor
Search :
Microsoft Academic Search
Cite

DOI: 10.18488/journal.57.2019.82.104.114

Selam Habtom , Sibhatu Gebrehiwot

Export to    BibTeX   |   EndNote   |   RIS

Akharaiyi, F., 2011. Antibacterial, phytochemical and antioxidant activities of datura metel. International Journal of Pharm Tech Research, 3(1): 478-483.

Arekemase, M.O., G.P. Oyeyiola and K.I. Balogun, 2013. Assessment of vernonia amygdalina on some selected pathogenic microorganisms from university of Ilorin teaching hospital. Journal of Microbiology, Biotechnology and Food Sciences, 2(5): 2360-2365.

Bauer, A., W. Kirby, J.C. Sherris and M. Turck, 1966. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45(4_ts): 493-496.Available at: https://doi.org/10.1093/ajcp/45.4_ts.493.

Bazie, S., A. Ayalew and K. Woldetsadik, 2014. Antifungal activity of some plant extracts against (colletotrichum musae) the cause of postharvest banana anthracnose. Journal of Plant Pathology and Microbiology, 5(2): 1-4.Available at: https://doi.org/10.4172/2157-7471.1000226.

Biruhalem, T., M. Giday, A. Animut and J. Seid, 2011. Antibacterial activities of selected medicinal plants in traditional treatment of human wounds in Ethiopia. Asian Pacific Journal of Tropical Biomedicine, 1(5): 370-375.Available at: https://doi.org/10.1016/s2221-1691(11)60082-8.

Cos, P., A.J. Vlietinck, D.V. Berghe and L. Maes, 2006. Anti-infective potential of natural products: How to develop a stronger in vitro ‘proof-of-concept. Journal of Ethnopharmacology, 106(3): 290-302.Available at: https://doi.org/10.1016/j.jep.2006.04.003.

Cowan, M.M., 1999. Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12(4): 564-582.Available at: https://doi.org/10.1128/cmr.12.4.564.

Dalal, H. and M. Alkhalifah, 2016. Antimicrobial activities of solanum incanum, elettaria cardamomum and zingiber officinale, used traditionally to treat pathogenicmicrobes. International Journal of Scientific Engineering and Applied Science, 2(3): 2395-3470.

Dewanjee, S., M. Kundu1, A. Maiti1, R. Majumdar, A.A. Majumdar, S.C. Mandal, D.J. Diekema, M.A. Pfaller, F.J. Schmitz, J. Smayevsky, J. Bell, R.N. Jones and M. Beach, 2007. Survey of infections due to staphylococcus species: Frequency of occurrence and antimicrobial susceptibility of isolates collected in the United States, Canada, Latin America, Europe, the Western Pacific region for the SENTRY Antimicrobial Surveillance Program 1997–1999. Clinical Infectious Diseases, 32(Suppl-2): S114-S132.

Eftekhar, F., M. Yousefzadi and V. Tafakori, 2005. Antimicrobial activity of datura innoxia and datura stramonium. Fitoterapia, 76(1): 118-120.Available at: https://doi.org/10.1016/j.fitote.2004.10.004.

Gao, Y., M.J. van Belkum and M.E. Stiles, 1999. The outer membrane of gram-negative bacteria inhibits antibacterial activity of brochocin-c. Applied and Environmental Microbiology, 65(10): 4329-4333.

Getaneh, G., A. Zemed, E. Abiyu and R. Nagappan, 2014. Ethnobotanical study of traditional medicinal plants and their conservation status in Mecha wereda, west Gojjam zone of Ethiopia. International Journal of Pharmaceutics and Health care Research, 02(03): 137-154.

Goel, A. and K. Sharma, 2014. Plant extracts and phytochemicals as herbal medicines and antimicrobials. International Journal Of Biological and Medical Research, 5(1): 3940-3946.

Hadia, G., R.N. Qaisrani, M.A. Khan, S. Hassan and N. Younis, 2012. Antibacterial and antifungal activity of different extracts of datura stramonium (branches and leaves sample). Journal of Biotechnology and Pharmaceutical Research, 3(9): 141-148.

Indhumathi, T. and S. Mohandass, 2014. Efficacy of ethanolic extract of solanum incanum fruit extract for its antimicrobial activity. International Journal of Current Microbiology and Applied Sciences, 3(6): 939-949.

Johnson, D.B., B. Shringi, D.K. Patidar, N.S.S. Chalichem and A.K. Javvadi, 2011. Screening of antimicrobial activity of alcoholic & aqueous extract of some indigenous plants. Indo-Global Journal of Pharmaceutical Sciences, 1(2): 186-193.

Kesatebrhan, H., 2013. Antimicrobial activity and phytochemical screening of crude extracts of medicinal plants grown in Eastern Ethiopia. International Journal of Pharmacy and Biological Sciences, 4(4): 326 - 333.

Nduagu, C., E. Ekefan and A. Nwankiti, 2008. Effect of some crude plant extracts on growth of Colletotrichum capsici (Synd) Butler & Bisby, causal agent of pepper anthracnose. Journal of Applied Biosciences, 6(2): 184-190.

Nikaido, H., 1996. Outer membrane. In: Neidhardt, F.C. (Ed.), Echerichia coli and salmonelle typhimiruim: Cellular and Molecular Bilogy. Washington, D.C: American Sociatety for Microbiology Press. pp: 29-47.

Owino, J., O. J. and S.N. Njeru, 2013. Antibacterial activity of methanolic crude extract of solanum incanum: Kenyan traditional medicinal plant. International Journal of Science and Research, 4(2): 2319-7064.

Parekh, J. and S. Chanda, 2007. In vitro antimicrobial activity and phytochemical analysis of some indian medicinal plants. Turkish Journal of Biology, 31(1): 53-58.

Roopashree, T., R. Dang, S. Rani and C. Narendra, 2008. Antibacterial activity of antipsoriatic herbs: Cassia tora, momordica charantia and calendula officinalis. International Journal of Applied Research in Natural Products, 1(3): 20-28.

Singh, U.P., H.B. Singh and R.B. Singh, 2007. The fungicidal effect of neem extracts on some soil borne pathogens of gram (cicer arietinum). Mycologia, 72(6): 1077-1093.

Solomon, G., 2015. Preliminary phytochemical screening and in vitro antimicrobial activity of datura stramonium leaves extracts collected from Eastern Ethiopia. International Research Journal of Biological Sciences, 4(1): 55-59.

Stepp, J.R., 2004. The role of weeds as sources of pharmaceuticals. Journal of Ethnopharmacology, 92(2-3): 163-166.Available at: https://doi.org/10.1016/s0378-8741(04)00104-7.

Terrier, B., D. Courtois, N. Hénault, A. Cuvier, M. Bastin, A. Aknin, J. Dubreuil and V. Pétiard, 2007. Two new disposable bioreactors for plant cell culture: The wave and undertow bioreactor and the slug bubble bioreactor. Biotechnology and Bioengineering, 96(5): 914-923.Available at: https://doi.org/10.1002/bit.21187.

Verma, S. and S. Singh, 2008. Current and future status of herbal medicines. Veterinary World, 1(11): 347-350.Available at: https://doi.org/10.5455/vetworld.2008.347-350.

World Health Organization (WHO), 2009. The use of herbal medicines in primary healthcare. Yangon, Myanmar: Report of the Regional Meeting, pp: 10-12.

Selam Habtom , Sibhatu Gebrehiwot (2019). In Vitro Antimicrobial Activities of Crude Extracts of Two Traditionally Used Ethiopian Medicinal Plants against Some Bacterial and Fungal Test Pathogens. The International Journal of Biotechnology, 8(2): 104-114. DOI: 10.18488/journal.57.2019.82.104.114
The aim of the present study was to assess the antimicrobial activities of crude extracts of Solanum incanum and Datura stramonium L. against four bacterial disease-causing species namely; Staphylococcus aureus, Streptococcus agalactiae, Salmonella typhi, Escherichia coli) and two fungal pathogenic species (Aspergillus flavus and Aspergillus niger). Crude extracts of roots of S. incanum and leaves of D. stramonium L. were prepared using absolute ethanol, methanol (99.8%) and distilled water. The potential efficacy resulting extracts were assessed by determining zone of inhibitions on cultures of six selected pathogens by applying disc diffusion method. The minimum inhibitory concentration (MIC) of the plant extracts against the same pathogens was determined using the broth dilution method. Chloroamphenicol and Tilt were used as positive controls for bacterial and fungal pathogens, respectively, while Dimethyl Sulfoxide was used as a negative control for both. The results showed that all the methanolic and ethanolic crude extracts, with the exception of the methanolic crude extract of D. stramonium, had the highest growth inhibitory effects as compared with the water crude extracts on all bacterial pathogens. The methanol crude extract of S. incanum had highest zone of inhibition (22±1.15 mm) against S. agalactiae with a minimum inhibitory concentration of 25mg/ml. Similarly, the methanol crude extract of S. incanum had shown significant antifungal activity against A. niger with inhibition zone of 25.13±0.4mm and a minimum inhibitory concentration of 125mg/ml. The results of the present study indicate the potential of these medicinal plants in treating some bacterial and fungal infection.
Contribution/ Originality
This study contributes in the existing literature by providing information regarding the antimicrobial (selected bacterial and fungal pathogens) efficacy potential of Solanum incanum and Datura stramonium crude extract.

Assessment of the Nutritional Qualities of a Locally-Produced Weaning Blend of Sorghum Ogi Flour Fortified with Bambara Groundnut Flour

Pages: 115-124
Find References

Finding References


Assessment of the Nutritional Qualities of a Locally-Produced Weaning Blend of Sorghum Ogi Flour Fortified with Bambara Groundnut Flour

Search :
Google Scholor
Search :
Microsoft Academic Search
Cite

DOI: 10.18488/journal.57.2019.82.115.124

Adeyemo Stella. M. , Abimbola Olufemi. G.

Export to    BibTeX   |   EndNote   |   RIS

Adeyemo, S. and A. Onilude, 2013. Enzymatic reduction of anti-nutritional factors in fermenting soybeans by Lactobacillus plantarum isolates from fermenting cereals. Nigerian Food Journal, 31(2): 84-90.Available at: https://doi.org/10.1016/s0189-7241(15)30080-1.

Adeyemo, S.M., 2012. Raffinose metabolism and utilization by L. plantarum isolates from indigenously fermented cereal gruels for nutritional improvement. Ph.D Thesis. Nigeria: University of Ibadan. pp 1-256.

Agren, G. and R. Gibson, 2008. Food composition table for use in Ethiopia. Addis Ababa, Ethiopia: Children’s Nutrition Unit. pp: 87-92.

Ajanaku, K., C. Ajanaku, A. Edobor-Osoh and O.C. Nwinyi, 2012. Nutritive value of sorghum ogi fortified with groundnut seed (Arachis) hypogaea. American Journal of Food Technology, 7(2): 82-88.Available at: https://doi.org/10.3923/ajft.2012.82.88.

American Association of Cereal Chemist, 2008. Technological handbook. Edited by M Carcea. 10th Edn., Methods. US: The Association, St. Paul, MN. pp: 44- 40.

Aminigo, G.O. and J.O. Akingbala, 2003. Nutritive composition and sensory properties of Ogi fortified with okra seed  meal. Journal of Applied Sciences and Environmental Management, 8(2): 23-30.

Association of Analytical Chemist (AOAC), 1990. Official methods of analysis. 10th Edn., Virginia, U.S.A: Association of Official Analytical Chemists. pp: 1012.

Aworh, O.C., 2008. The role of traditional food processing technologies in national development: The West African experience. International Union of Food Science and Technology, 1(6): 1-18.

Awoyade, W., M. Busie and M.A. Abebe, 2016. Rentention of pro-vitamin a carotenoid in Ogi powder as affected by packaging materials and storage conditions. Journal of Food and Nutrition Research, 4(2): 88-93.

Bamshaiye, O., J. Adegbola and E. Bamishaiye, 2011. Bambara groundnut: An under-utilized nut in Africa. Advances in Agricultural Biotechnology, 1(7): 60-72.

Brough, S.H. and S.N. Azam- Ali, 2003. The effect of soil moisture on the proximate composition of Bambara groundnut (Vigna subterranea (L) Verdc.) Journal of Food, Science  and Agriculture, 60: 197-203.

Edema, M.O., L.O. Sanni and A.I. Sanni, 2005. Evaluation of Maize-soybean flour blends for sour maize bread production in Nigeria. African Journal of Biotechnology, 4(9): 911-917.

Mbata, T.I., M.J. Ikenebomeh and I. Ahonkhai, 2007. Nutritional status of maize fermented meal by fortification with bambara-nut. African Journal of Food Agriculture Nutrition. Development, 7(2): 1-14.

Mbata, T.I., M.J. Ikenebomeh and J. Alaneme, 2009. Studies on the microbiological, nutrient composition and antinutritional contents of fermented maize flour fortified with bambara groundnut (vigna subterranean l). African Journal of Food Science, 3(6): 165-171.

Mugendi, J.B., E. Njagi, E. Kuria, M. Mwasaru, J. Mureithi and Z. Apostolides, 2010. Effects of processing techniques on the nutritional composition and anti-nutrient content of mucuna bean (mucuna pruriens l). African Journal of Food Science, 4(4): 156-166.

Nishant, T., K.D. Sathish, K.R. Arun, K. Himabindu and Y. Raviteja, 2011. Bacteriocin producing probiotic lactic acid bacteria. Journal of Microbial And Biochemical Technology, 3(5): 121-124.Available at: https://doi.org/10.4172/1948-5948.1000062.

Oguntona, C.R. and I.O. Akinyele, 2002. Food and nutrient intakes by pregnant Nigerian adolescents during the third trimester. Nutrition, 18(7-8): 673-679.Available at: https://doi.org/10.1016/s0899-9007(02)00747-5.

Okorie, D.O., C.O. Eleazu and P. Nwosu, 2015. Nutrient and heavy metal composition of platain (Musa paradisiaca) and banana (Musa paradisiaca) peels. Journal of Nutrition and Food Science, 5: 370-377.

Onilude, A.A., A.I. Sanni and M.I. Ighalo, 2004. Process upgrade and the microbiological, nutritional and consumer acceptability of infant weaning food from composite blends of cereals and soybean. Journal of Food Agriculture and Environment, 2(7): 64-68.

Sanni, A., M. Asiedu and G. Ayernor, 2001. Influence of processing conditions on the nutritive value of Ogi-baba, a Nigerian fermented sorghum gruel. Plant Foods for Human Nutrition, 56(3): 217-223.

Snedecore, G.W., A. Aminat, I.W. Mostafa and J. Rossi, 2015. Phytochemical Composition  and Antioxidant  Properties of Indigenous Landraces of OMANI Fenugreek seeds. Complementary Alternative Medicine, 1292: 149-154.

Wakil, S.M. and M.O. Kazeem, 2012. Assessment of weaning food produced from feremented cereal-legume blends using starters. International Food Research Journal, 19(4): 1679-1685.

Wakil, S.M. and A.A. Onilude, 2009. Microbiological and chemical changes during production of malted and fermented cereal-legume weaning foods. Advances in Food Sciences by PSB, 31(9): 139-145.

Zakari, U., A. Hassan and E. Abbo, 2010. Physico-chemical and sensory properties of “agidi” from pearl-millet (pennisetum glaucum) and bambara groundnut (vigna subterranean) flour blends. African Journal of Food Science, 4(10): 662-667.

No any video found for this article.
Adeyemo Stella. M. , Abimbola Olufemi. G. (2019). Assessment of the Nutritional Qualities of a Locally-Produced Weaning Blend of Sorghum Ogi Flour Fortified with Bambara Groundnut Flour. The International Journal of Biotechnology, 8(2): 115-124. DOI: 10.18488/journal.57.2019.82.115.124
This study investigated the nutritional quality of a locally-produced weaning blend of sorghum ogi flour fortified with Bambara groundnut (BGN) flours and the role of lactic acid bacteria (LAB) as starter in the reduction of anti-nutrients in the pre-treated BGN flours. LAB were isolated from fermented sorghum ogi, processed into flour. BGN were processed into flours using three pre-treatment methods and used to fortify the sorghum ogi flour in ratio 60:40. Proximate composition and the breakdown of anti-nutritional factors by LAB were monitored in the pre-treated BGN flours using standard procedures. Sensory, organoleptic attributes and shelf-life of the weaning blends were determined. Sorghum ogi flour fortified with roasted BGN flour had improved nutritional value; protein, ether extract, ascorbic acid, riboflavin, ?-carotene, calcium, and phosphate (mg/100g) 20.16, 6.43, 21.00, 0.13, 90.00, 145.00 and 280.00 respectively compared to boiled and soaked BGN flours. There was a reduction in the anti-nutritional factors of the pre-treated BGN flours when fermented with selected starter. Tannin, phytate, protease and trypsin inhibitors reduced significantly (p< 0.05) from 0.074 - 0.048; 5.901- 5.001; 0.052 - 0.043 and 0.137 -0.110 respectively. The shelf-life monitoring and organoleptic assessment showed that sorghum Ogi flour fortified with roasted BGN flour had a prolonged shelf life and was generally accepted when compared to other weaning blends. The study concluded that the weaning blend had improved nutritional composition required for a growing infant. LAB could be used as starters to reduce anti-nutritional factors and extend the shelf-life of pre-treated Bambara groundnut-sorghum ogi blends.
Contribution/ Originality
The paper’s primary contribution is finding that adequate weaning food can be prepared locally with minimal funds. The blend is able to meet the daily nutritional requirement of a growing infant. It also advances the contribution of women especially in low economic groups in reducing malnutrition and infant mortality rate.