The Asia Journal of Applied Microbiology

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Online ISSN: 2313-8157
Print ISSN: 2409-2177
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Domestic Wastewater Treatment and Lipid Accumulation for Biodiesel Production by an Isolated Heterotrophic Microalgae from an Arid Climate Zone

Pages: 1-9
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Domestic Wastewater Treatment and Lipid Accumulation for Biodiesel Production by an Isolated Heterotrophic Microalgae from an Arid Climate Zone

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DOI: 10.18488/journal.33.2019.61.1.9

Abdelghani El Asli , Nadia El Mesbahi , Rachida Oubakalla , Loubna El Fels , Mohamed Hafidi , Yanna Liang

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Abdelghani El Asli , Nadia El Mesbahi , Rachida Oubakalla , Loubna El Fels , Mohamed Hafidi , Yanna Liang (2019). Domestic Wastewater Treatment and Lipid Accumulation for Biodiesel Production by an Isolated Heterotrophic Microalgae from an Arid Climate Zone. The Asia Journal of Applied Microbiology, 6(1): 1-9. DOI: 10.18488/journal.33.2019.61.1.9
The isolation of microalgae that can use carbon-rich pollutant from wastewater and accumulate lipids is of great interest in biodiesel production. The heterotrophic cultivation of microalgae could overcome light dependency, and hence increase the yield of microalgae lipid per unit area. After several microbial screening and acclimation procedures, strains of microalgae were proved to be tolerant of wastewater and to grow heterotrophically. One top-performing strain (AE2) was identified through morphological observation as Chlorella sp and was isolated from an open pond of domestic wastewater. This microalgae is able to grow in raw wastewater at 20°C with no illumination and eliminates 100% of its COD (424 mg/L) in 9 days. The biomass produced in wastewater as growth medium comprises 53% of fats of the dry mass and 68% of fats of the dry mass in BG 11 culture medium supplemented with 5 g of glucose/L. The analysis of fatty acid methyl esters FAME composition was 32.5 % of the total biomass. The extracted microalgae oil was converted to good quality biodiesel. This heterotrophic isolated microalgae is a promising strain in terms of wastewater COD removal and cytoplasmic accumulation of high quality and quantity lipids for prospective biodiesel production.
Contribution/ Originality
This study is one of the very few studies which have investigated the isolation of microalgae from the southern climate that can grow efficiently in low carbon sources such as wastewater. The isolated microalgae produced biomass with 53% of fats that are favorable in term quantity and quality for biodiesel conversion.

Substrate Serving for Culture Optimization and Protease Productivity by Penicillium Notatum Strain on Deproteinised Foliage Extract from Lucerne and Beet

Pages: 10-17
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Substrate Serving for Culture Optimization and Protease Productivity by Penicillium Notatum Strain on Deproteinised Foliage Extract from Lucerne and Beet

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DOI: 10.18488/journal.33.2019.61.10.17

Rajesh K. Jadhav

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Rajesh K. Jadhav (2019). Substrate Serving for Culture Optimization and Protease Productivity by Penicillium Notatum Strain on Deproteinised Foliage Extract from Lucerne and Beet. The Asia Journal of Applied Microbiology, 6(1): 10-17. DOI: 10.18488/journal.33.2019.61.10.17
When Penicillium notatum and Aspergillus niger grown on Lucerne DPJ by serving 1 % and 2 % of the substrates, viz., casein, starch and carboxymethyl cellulose (CMC), it showed the promising results of mycelial biomass and hydrolytic enzyme activities. Present attempt was on growth of species Penicilium notatum and Aspergillus niger on beet and lucerne foliage DPJ broth medium as compared with control glucose nitrate (GN) medium. Aspergillus niger not showed its growth on DPJ when enriched with casein and starch but there was growth on DPJ alone when substrates were not added. Therefore DPJ itself has the efficacy in initiating fungal growth. Beet DPJ inhibited the growth of Penicillium notatum when substrates were added, and only 1% of CMC was found responsible to initiate its growth by addition in DPJ. Penicillium notatum thrieved well on lucerne DPJ. Increasing concentration of casein in Lucerne DPJ enhanced rate of enzyme protease. Beet DPJ found having its antimicrobial influence. The objective of research was optimization of enzyme productivity by DPJ for industrial use.
Contribution/ Originality
This study is one of few studies performed by the influence of deproteinised leaf extract from lucerne and beet leaves by adding the substrates in it and subjecting it for the estimation of enzymes. The secondary metabolites can be having the quality of specific herbal influence along with the fungal effects like antibiotics or the vitamins.