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Ovitrap Surveillance of the Dengue Vectors Aedes aegypti and Aedes albopictus in Selected Endemic Areas in Sandakan, Sabah

Mohd Amierul Fikri M


Faizul Akmal AR


Mohd Farihan MY

Mohd Amierul Fikri M 1 ,

Faizul Akmal AR 1 Mohd Farihan MY 1 
  1. Institute For Public Health, National Institute of Health, Ministry of Health Malaysia. 1

Pages: 1-7

DOI: 10.18488/journal.106.2021.61.1.7

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Article History:

Received: 04 January, 2021
Revised: 27 January, 2021
Accepted: 10 February, 2021
Published: 22 February, 2021


Ovitrap technique could be considered an efficient tool for monitoring of Aedes populations. This study aimed to monitor the dengue vector population and to provide baseline data of vector density in selected endemic areas. 1600 ovitraps were placed indoor and outdoor randomly in selected houses from January till July 2014 at eight selected localities. 96%–100% of ovitraps were recovered after seven days. In total, 9399 larvae were examined of which 2674 (28.2%) were Ae. Aegypti and 6752 (71.8%) Ae. albopictus. Ae. albopictus were dominant than Ae. aegypti in every locality. Highest collection of Ae. albopictus was recorded in Kampung Air (total larvae = 1357), followed by Kampung Bokara (total larvae = 1165) and Taman Fajar (total larvae = 846). Three main localities that recorded highest collection of Ae. aegypti were Kampung Bokara (total larvae = 504), Kampung Air (total larvae = 457) and Taman Fajar (total larvae = 341). Ae. albopictus was found more abundant compared to Ae. aegypti. The ovitrap index recorded in all localities (ranged 18.0% - 59.0%) was more than the transmission threshold (10%). Mix breeding of Ae. aegypti and Ae. albopictus were also detected for outdoor ovitraps. In conclusion, this study found that selected localities in Sandakan district have a high risk for transmission and outbreaks of dengue. Furthermore, this study also provided a baseline data of Aedes density for further planning of prevention and control activities.
Contribution/ Originality
This study documents the ovitrap surveillance as an important tool for management and planning of dengue vector strategy focusing on endemic areas.


Ovitrap, Aedes aegypti, Aedes albopictus, Dengue, Vector-borne, Malaysia.


[1]          A. Kroeger and M. B. Nathan, "Dengue: Setting the global research agenda," The Lancet, vol. 368, pp. 2193-2195, 2006.Available at:

[2]          A. H. Mohd-Zaki, J. Brett, E. Ismail, and M. L'Azou, "Epidemiology of dengue disease in Malaysia (2000–2012): A systematic literature review," PLoS Negl Trop Dis, vol. 8, p. e3159, 2014.

[3]          C. C. d. A. Marques, G. R. d. A. M. Marques, M. d. Brito, L. G. d. Santos Neto, V. d. C. Ishibashi, and F. d. A. Gomes, "Comparative study of the efficiency of larval and ovitraps for the surveillance of dengue and yellow fever vectors," Public Health Magazine, vol. 27, pp. 237-241, 1993.

[4]          I. A. Braga, A. d. C. Gomes, M. Nelson, R. d. C. G. Mello, D. P. Bergamaschi, and J. M. P. d. Souza, "Comparison between larval research and oviposition trap for the detection of Aedes aegypti," Journal of the Brazilian Society of Tropical Medicine, vol. 33, pp. 347-353, 2000.Available at:

[5]          H. Rozilawati, K. Tanaselvi, W. Nazni, S. Mohd Masri, J. Zairi, C. Adanan, and H. Lee, "Surveillance of aedes albopictus skuse breeding preference in selected dengue outbreak localities, peninsular Malaysia," Tropical Biomedicine, vol. 32, pp. 49-64, 2015.

[6]          H. L. Lee, "Aedes ovitrap and larval survey in several suburban communities in Selangor Malaysia," Mosquito-Borne Diseases Bulletin, vol. 9, pp. 9-15, 1992.

[7]          D. A. Focks, "A review of entomological sampling methods and indicators for dengue vectors," Dengue Bull, vol. 28, pp. 10-34, 2000.

[8]          M. Service, "Importance of ecology in Aedes aegypti control," The Southeast Asian journal of Tropical Medicine and Public Health, vol. 23, pp. 681-690, 1992.

[9]          A. Tham, Surveillance of mosquitoes. In: F.S.P. Ng & H.S. Yong, eds. Mosquito and Mosquito Borne Disease. Kuala Lumpur: Malaysian Academy of Sciences, 2000.

[10]        L. L. N. Serpa, G. R. A. M. Marques, A. P. de Lima, J. C. Voltolini, M. de Brito Arduino, G. L. Barbosa, V. R. Andrade, and V. L. C. de Lima, "Study of the distribution and abundance of the eggs of Aedes aegypti and Aedes albopictus according to the habitat and meteorological variables, municipality of São Sebastião, São Paulo State, Brazil," Parasites & Vectors, vol. 6, pp. 1-11, 2013.

[11]        N. Wijegunawardana, Y. Gunawardene, T. Chandrasena, R. Dassanayake, N. Udayanga, and W. Abeyewickreme, "Evaluation of the effects of Aedes vector indices and climatic factors on dengue incidence in Gampaha District, Sri Lanka," BioMed Research International, vol. 2019, pp. 1-11, 2019.

[12]        MOH, "Guidelines on the use of ovitraps for aedes surveillance," ed Malaysia: Ministry of Health Malaysia, 1997, pp. 1-20.

[13]        S. Mahadevan, W. Cheong, and A. Hassan, Vectors of dengue and dengue haemorrhagic fever in West Malaysia. Kuala Lumpur: Institute for Medical Research, 1973.

[14]        D. P. O. De Melo, L. R. Scherrer, and Á. E. Eiras, "Dengue fever occurrence and vector detection by larval survey, ovitrap and MosquiTRAP: A space-time clusters analysis," PloS One, vol. 7, p. e42125, 2012.

[15]        A. Noor Afizah, W. Mohd Arif, H. L. Lee, and A. Nazni, "Ovitrap surveillance of Aedes aegypti and Aedes albopictus in dengue endemic areas in Keramat and Shah Alam, Selangor in 2016," IIUM Medical Journal Malaysia, vol. 17, pp. 59-64, 2018.

[16]        S. Snr, Y. Norma-Rashid, and M. Sofian-Azirun, "Mosquitoes larval breeding habitat in urban and suburban areas, Peninsular Malaysia," World Acad Sci Eng Technol, vol. 58, pp. 569-573, 2011.

[17]        H. L. Lee, "A nationwide resurvey of the factors affecting the breeding of Aedes aegypti (L.) and Aedes albopictus (Skuse)(Diptera: Culicidae) in urban towns of peninsular Malaysia-1988-1989," Trop Biomed, vol. 8, pp. 57-60, 1991.

[18]        M. Chang and N. Jute, "Distribution and density of aedes aegypti and aedes ALBOPICTUS (SKUSE) in SARAWAK," Med. J. Malaysia, vol. 37, pp. 205-210, 1982.

[19]        M. Mahmud, H. Mutalip, N. Lodz, and H. Shahar, "Study on key Aedes spp breeding containers in dengue outbreak localities in Cheras district, Kuala Lumpur," International Journal of Mosquito Research, vol. 5, pp. 23-30, 2018.

[20]        M. R. Dibo, F. Chiaravalloti-Neto, M. Battigaglia, A. Mondini, E. A. Favaro, A. A. Barbosa, and C. M. Glasser, "Identification of the best ovitrap installation sites for gravid Aedes (Stegomyia) aegypti in residences in Mirassol, state of São Paulo, Brazil," Memórias do Instituto Oswaldo Cruz, vol. 100, pp. 339-343, 2005.

[21]        C. J. Beech, J. Nagaraju, S. Vasan, R. I. Rose, R. Y. Othman, V. Pillai, and T. Saraswathy, "Risk analysis of a hypothetical open field release of a self-limiting transgenic Aedes aegypti mosquito strain to combat dengue," Asia Pacific Journal of Molecular Biology and Biotechnology, vol. 17, pp. 97-108, 2009.

[22]        N. Syarifah, T. Rusmatini, T. Djatie, and F. Huda, "Ovitrap ratio of Aedes aegypti larvae collected inside and outside houses in a community survey to prevent dengue outbreak, Bandung, Indonesia, 2007," Proc Assoc Southeast Asian Nations Congr Trop Med Parasitolol, vol. 3, pp. 116-120, 2008.

[23]        R. Norzahira, O. Hidayatulfathi, H. Wong, A. Cheryl, R. Firdaus, H. Chew, K. Lim, K. Sing, M. Mahathavan, and W. Nazni, "Ovitrap surveillance of the dengue vectors, Aedes (Stegomyia) aegypti (L.) and Aedes (Stegomyia) albopictus Skuse in selected areas in Bentong, Pahang, Malaysia," Tropical biomedicine, vol. 28, pp. 48-54, 2011.


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Authors would like to extend their gratitude to the staff of vector-borne unit, Sandakan Health District Office, Sabah for their assistance and support in this study.

Competing Interests:

The authors declare that they have no competing interests.


Authors would like to thank the Director General of Health, Malaysia for his permission to publish this paper.

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