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Comparative Evaluation of Six Different Storage Materials for Long-Term Preservation of Huckleberry (Solanum scabrum) Seeds

Maurice Njiandoh Mbeboh


Emmanuel Youmbi


William Armand Mala


Gabriel Ambroise Manga


Amos Funamo Ngwa


Sylvestre Medard Badjel Badjel


Christopher Ngosong

Maurice Njiandoh Mbeboh 1

Emmanuel Youmbi 2 William Armand Mala 2 

Gabriel Ambroise Manga 4 Amos Funamo Ngwa 4 Sylvestre Medard Badjel Badjel 4 Christopher Ngosong 7

  1. Department of Plant Biology and Physiology, Faculty of Science, University of Yaounde 1 and Department of Agronomic and Applied Molecular Sciences, Faculty of Agriculture and Veterinary Medicine, University of Buea, Buea, South West Region, Cameroon. 1

  2. Department of Plant Biology and Physiology, Faculty of Science, University of Yaounde 1, Yaounde, Centre Region, Cameroon. 2

  3. Institute of Agricultural Research for Development (IRAD), Njombe, Littoral Region, Cameroon. 4

  4. Department of Agronomic and Applied Molecular Sciences, Faculty of Agriculture and Veterinary Medicine, University of Buea, and Leibniz Institute of Vegetable and Ornamental Crops, Großbeeren, Germany. 7

Pages: 30-38

DOI: 10.18488/journal.70.2020.71.30.38

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Article History:

Received: 03 September, 2019
Revised: 07 October, 2019
Accepted: 14 November, 2019
Published: 12 December, 2019


Smallholder farmers in Cameroon often store seeds from their own-harvest for use in subsequent planting seasons, but they have limited information on appropriate materials for long-term storage of vegetable seeds. Hence, six different materials (sealed aluminium foil sachet, sealed glass bottle, closed plastic cup, sealed paper sachet, sealed polythene sachet, and open-and-seal glass bottle) were evaluated for their effectiveness as storage materials for huckleberry seeds. Factorial ANOVA revealed significant (P < 0.001) effect of the different types of storage materials, duration of seed storage, and their interactions on laboratory germination, field emergence, and percentage transplantable see dlings. Significant (P < 0.05) variations were observed for laboratory seed germination and field emergence from months 2–5 of preservation across the different storage materials, with the highest performance exhibited by sealed aluminium foil sachet, while glass material exhibited the lowest performance. These performances decreased significantly (P < 0.05) for each storage material across months of long-term storage, with the lowest decrease recorded in sealed aluminium foil sachet and highest decrease recorded in glass bottle storage materials. Percentage transplantable seedlings decreased significantly (P < 0.05) from month 0–5 for each storage material, with lowest decrease in sealed aluminium foil sachet and highest decrease in open-and-seal glass bottle. Overall, these results demonstrate that sealed aluminium foil sachet is the most effective storage material for long-term preservation of huckleberry seeds under ambient conditions of the study area, which can be adopted by farmers.
Contribution/ Originality
This study is one of very few studies that have investigated different storage materials for long-term preservation of huckleberry seeds in Cameroon, and demonstrated that sealed aluminium foil sachet is the most effective under ambient conditions in the study area, which can be adopted by smallholder farmers.


African leafy vegetables, Aluminium foil, Field emergence, Germination, Glass bottle, Nightshade, Seed systems, Storage materials.



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The study was supported by Institute of Agricultural Research for Development (IRAD) Cameroon, and Integrated Soil Fertility Management (ISFM) research group of the Faculty of Agriculture and Veterinary Medicine, University of Buea via Research and Modernization Grants of the Ministry of Higher Education Cameroon. CN was supported by the Alexander von Humboldt Foundation, Germany.

Competing Interests:

The authors declare that they have no competing interests.


All authors contributed equally to the conception and design of the study.

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