The influence of seleno-cystine (CySe2) added to ovine ruminal fluids containing α-linolenic acid (αLNA) on the profile of fatty acids (FA) was investigated. Fluids were incubated in vitro at 39°C under CO2 either alone (RF) or with αLNA (1.67 mg/ml) or with a combination of αLNA with either a low (1.34 μg/ml) or high (3.33 μg/ml) level of Se as CySe2. Fluids were removed after 0, 6, 12, 18, 24 hrs of incubation and then analyzed to determine FA levels. αLNA added to the fluids without/with CySe2 decreased the C18α0 concentration for incubation at all times from 6 hrs compared with the RF or the fluid containing CySe2. αLNA added to the fluids without/with CySe2 decreased the biohydrogenation yield to C18:0. CySe2 added to the fluids decreased the C18:0 concentration and the index of the biohydrogenation to C18:0 compared with the RF. The higher concentration of CySe2 in the fluids with αLNA reduced the accumulation of trans11C18:1 for incubation at all times from 18 hrs compared with the fluids with αLNA, irrespective of the presence of the lower concentration of CySe2. The lowest concentration of trans11C18:1 in the fluids with LNA and the higher concentration of CySe2 correlated with the lowest yield of the isomerization of αLNA into cis9trans11cis15C18:3 and the lowest yield of the initial biohydrogenation of cis9trans11cis15C18:3 to trans11cis15C18:2 in the fluids containing αLNA and the higher concentration of CySe2. CySe2 added to the fluids with αLNA decreased the ratio of polyunsaturated FA to saturated FA for incubation at all times from 12 hrs compared with the fluids containing αLNA. CySe2 in the fluids without/with αLNA reduced the FA sum in the fluids.
Our original study documents that CySe2 added to the ovine ruminal fluids, irrespective of the presence of αLNA, affects concentrations of fatty acids, the capacity of the bacterial isomerases and the biohydrogenation yield of unsaturated fatty acids in in vitro incubated ruminal fluids compared with the control fluid.
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