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Current Research in Agricultural Sciences

June 2017, Volume 4, 2, pp 51-60

Molecular Characterization of Parental Lines of Rice Aiming to Address High Yield and Nutritional Quality Under Drought and Cold Stress Condition

Nomita Das

,

Partha S. Biswas

Nomita Das 1
Partha S. Biswas 2


  1. Jahangirnagar University, Savar, Dhaka, Bangladesh 1

  2. Bangladesh Rice Research Institute, Gazipur, Bangladesh 2

Pages: 51-60

DOI: 10.18488/journal.68.2017.42.51.60

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Article History:

Received: 02 March, 2017
Revised: 30 March, 2017
Accepted: 03 May, 2017
Published: 07 June, 2017


Abstract:

Abiotic stresses limit crop growth at different growth stages resulting low yield of rice. Molecular characterization of parental materials gives precise information on the extent of genetic diversity exists between them. A set of 60 SSRs randomly distributed over 12 chromosomes were used to analyze eight cultivars intend to be used as parent in breeding programs to address cold and drought tolerance, and nutritional quality of rice. A total of 300 alleles were detected across the cultivars for 51 polymophic markers with 5.88 alleles per loci. On average 30.6% of the genotypes shared a common allele at any given locus. UPGMA cluster analysis showed that 67% common alleles were shared by the cultivars. The cultivars were clearly grouped into two distinct clusters at 67.0% genetic similarity. Hbj. BVI showing high tolerance to cold stress at seedling stage differed from cold susceptible BR1, BRRI dhan28 and BRRI dhan29 by 33% alleles while BR18 that showed moderately cold tolerance differed by 29.0 - 29.9% alleles which indicated that only 3-4% alleles difference caused higher cold tolerance in Hbj. BVI. The moderate genetic distance between cold tolerant Hbj. BVI and high yielding BRRI dhan28 and BRRI dhan29 indicated that there is higher possibility of obtaining high yielding cold tolerant segregants from the crosses between them. On the other hand,  Kalobokri, which had 31.7 mg zinc a kilogram of polished rice differed by 33.0% alleles from the drought tolerant rainfed low varieties BRRI dhan56 and BRRI dhan57 having moderate level of zinc (~20 mg/kg), which also indicated that crosses between them might produce progenies with higher nutritional quality under drought environment.

Contribution/ Originality
This study is one of the few studies which have investigated the genetic distance between the parental lines that are intended to be used in the breeding program to address abiotic stresses, like drought and cold tolerance with high yield potential and enhanced nutritional quality, particularly zinc content in rice.  

Keywords:

Drought, Cold, Grain zinc content, Genetic diversity, SSR, Rice.

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Reference:

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Funding:

This study received no specific financial support.

Competing Interests:

The authors declare that they have no competing interests.

Acknowledgement:

We acknowledge Bangladesh Rice Research Institute for providing laboratory support for this study. We are also grateful to the scientists and staffs working in the molecular laboratory of Plant Breeding Division in BRRI. We express our gratitude to BRRI Ge

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( 148 ) Comparative Growth Analysis and Yield Performance of Glycine Max under Jatropha Curcas Based Agrisilviculture System of Agroforestry in the Northern Part of Bangladesh
( 149 ) Correlation between Milk Yield, Somatic Cell Count and Milk Quality in Dairy Farming
( 151 ) Genotype X Environment Interaction and Stability Analysis for Yield and Yield Related Traits of Desi-Type Chickpea (Cicer Arietinum L.) In Ethiopia
( 152 ) Effect of Intra Spacing on Yield and Yield Components of Carrot (Daucus Carrota L.Sub Sp. Sativus)
( 153 ) Genotypic Difference in Growth and Yield Related Traits of Onion (Allium Cepa L.) Varieties at Southern Tigray
( 156 ) Evaluation of Different Tillage Practices on Growth and Yield of Fluted Pumpkin Telfairia Occidentalis in Uyo, Southeastern Nigeria
( 157 ) Heterosis for Nitrogen Fixation and Seed Yield and Yield Components in Chickpea (Cicer arietinum L.)
( 159 ) Harvesting Date Influences Cassava (Manihot Esculenta Crantz) Yield and Quality of Based-Products
( 160 ) Uncertainty Analysis and Calibration of Swat Model for Estimating Impacts of Conservation Methods on Streamflow and Sediment Yield in Thika River Catchment, Kenya
( 161 ) The Impact of Intensive Utilization of Cultivated Land on Grain Yields: A Case Study of Shandong Province in China
( 162 ) Genotype-Environment Interactions and Yield Stability of Cowpea (Vigna Unguiculata L. Walp) in Lomami Province, Central Part of Democratic Republic of Congo
( 163 ) Effect of Blended Fertilizer and Lime Application on Yield of Finger Millet, and Soil Properties of Acidic Soils in Western Ethiopia
( 164 ) Physiological Aspects of Yield Variation among Seven Groundnut Genotypes Cultivated under Rain-Fed Conditions
( 165 ) Effect of Planting Date on Growth and Yield of Bambara Groundnut (Vigna subtermnea (L) Verdc.) Varieties in Rainforest Zone of Delta State
( 168 ) Insect Activities and their Impact on the Yield of Abelmoschus esculentus L ( Malvaceae) in Bambili ( Mezam - Cameroon)
( 169 ) On-Farm Evaluation of Conservation Agriculture Practice on Weed Control and Yield of Wheat in Northern Bangladesh
( 170 ) Nutritional Properties of Some Novel Selected Fish Species in Khuzestan Province, Iran