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Journal of Food Technology Research

June 2016, Volume 3, 2, pp 99-104

Design of Experiment - Optimization of HPLC Analysis of Vitamin A and E in Margarine and Vegetable Oil

Lorinc Garai

Lorinc Garai 1

  1. Doctoral School of Multidisciplinary Engineering Sciences, Szechenyi University; Gyor, Hungary 1

on Google Scholar
on PubMed

Pages: 99-104

DOI: 10.18488/journal.58/2016.3.2/58.2.99.104

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Article History:

Received: 16 November, 2016
Revised: 22 December, 2016
Accepted: 30 December, 2016
Published: 09 January, 2017


Abstract:

Liquid chromatography is a useful method for selective analysis of vitamin A and E. The native components can be easily tested due to their UV-absorbance and beneficial separation characteristics. To test the food sample, vitamin A and E have to be separated from the fatty part of food. This step is performed by saponification, which is the critical step of procedure due to the heat and light sensitivity of both vitamins. The corresponding standards (MSZ-EN 12822-1, MSZ EN 12823-1) recommend a wide range of saponification temperature and time, giving an opportunity to optimize the recovery of the analyte. Experimental tests were performed from vegetable oil and margarine sample to find the temperature and time settings for the best recovery. Both factors were examined with centered two-level factorial design. Data analysis showed an increasing recovery of analytes towards the corner points of highest possible temperature and shortest possible saponification time. Finally, confirmatory tests were made.
Contribution/ Originality

Keywords:

HPLC, Vitamin A, Vitamin E, Experimental design, Recovery.

Video:

Reference:

  1. Gimeno, E., A.I. Castellote, R.M. Lamuela-Raventós, M.C. De la Torre and M.C. López-Sabater, 2000. Rapid determination of vitamin E in vegetable oils by reverse-phase high-performance liquid chromatography. Journal of Chromatography A, 881(1-2): 251-254. View at Google Scholar | View at Publisher
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  3. Nollet, L.M.L., 1996. Handbook of food analysis. Marcel Dekker Inc, 1: 611-615.
  4. Thompson, J., G. Hatina and W. Maxwell, 1980. High performance liquid chromatographic determination of vitamin A in margarine, milk, partially skimmed milk, and skimmed milk. Journal - Association of Official Analytical Chemists, 63(4): 894-898. View at Google Scholar 
  5. Xu, Z., 2008. Comparison of extraction methods for quantifying vitamin E from animal tissues. Bioresource Technology, 99(18): 8705-8709. View at Google Scholar | View at Publisher

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Funding:

This study received no specific financial support.

Competing Interests:

The author declares that there are no conflicts of interests regarding the publication of this paper.

Acknowledgement:


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( 301 ) Remotion of Hydrogen Sulfide in Absorption Column
( 302 ) The Evaluation of the Agriculture of Hazelhazelnut in Duzce According to Climate Parameters
( 303 ) Status of Integrated Soil Fertility Management (ISFM) in Southwestern Nigeria
( 304 ) The Domestic Livestock Resources of Turkey: Silkworm (Bombyx Mori, L. 1758) (Lepidoptera: Bombycidae) Lines and their Conservation
( 305 ) Evaluation of Different Tillage Practices on Growth and Yield of Fluted Pumpkin Telfairia Occidentalis in Uyo, Southeastern Nigeria
( 306 ) Accelerating Agricultural Productivity to Enhance Economic Growth in Botswana
( 307 ) An Assessment of the Market Structure of Sesame among Farmers, Wholesalers and Retailers in Nasarawa State, Nigeria
( 308 ) Heterosis for Nitrogen Fixation and Seed Yield and Yield Components in Chickpea (Cicer arietinum L.)
( 311 ) Mechatronics and Precision Livestock Farming in Mexican Animal Production
( 312 ) Stage Specific Life Table of Invasive Pest Mealybug, Phenacoccus Solenopsis (Tinsley) Under Cotton Field Conditions
( 313 ) Molecular Characterization of Parental Lines of Rice Aiming to Address High Yield and Nutritional Quality Under Drought and Cold Stress Condition
( 314 ) Perception of Cassava Farmers of Climate Change on Cassava Production in Ido Local Government Area of Oyo State, Nigeria
( 315 ) Evaluation of the Sustainability Criteria of Forest Management in the Municipality Palma Soriano, Santiago De Cuba Province
( 319 ) Molecular Studies on E.Coli Isolate from Milk of Mastatic Cattle with Special Reference to Associated Biochemical Changes in Kaliobea Governorate
( 320 ) Improvement of Maize Productivity (Zea Mays L.) by Mycorrhizal Inoculation on Ferruginous Soil in Center of Benin
( 321 ) Role of Probiotics in Animal Nutrition
( 322 ) Management of Food Shelf Life and Energy Efficiency with Adaptive Food Preservation System (AFPS) Appliance
( 323 ) Prospects of Rural Youths Participation in Family Farming in Benue State, Nigeria: Implications for Policy
( 324 ) Feeding Potential of Red Palm Weevil, Rhynchophorus Ferrugineus on Different Date Palm, Phoenix Dactylifera Varieties Under Field Conditions
( 325 ) Establishing Short Bowel Syndrome in Nigerian Dogs
( 326 ) Helminths and Protozoa of the Gastrointestinal Tract of Ruminants in Tanzania
( 327 ) Growth and Survival Parameters and Blood IgG and Total Protein Levels of Calves Born in the First Production Year of Brown Swiss and Simmental Cows
( 328 ) Assessment of Government Feeding Programme on Improved Nutritional Health Status of Secondary School Students in Maiduguri, Borno State
( 329 ) Variation of Wood Density in Tropical Rainforest Trees
( 330 ) Forest Ecosystem Service Valuation: A Case of the Kalika Community Forest, Dhading, Central Nepal
( 331 ) Adaptation Study of Mung Bean (Vigna Radiate) Varieties in Raya Valley, Northern Ethiopia
( 333 ) Effect of Soaking on the Nutritional Values of Kordala (Maerua Pseudopetalosa) Seeds Grown in Kordofan Region, Sudan
( 334 ) Assessment of the Physical and Chemical Properties of Three Contrasting Soils Under Different Land Use Systems
( 335 ) Palm Kernel Separation Efficiency and Kernel Quality from Different Methods Used in Some Communities in Rivers State, Nigeria
( 336 ) Performance Evaluation of Onion (Allium Cepa L.) Varieties at Benatsemay Woreda of South Omo Zone, Ethiopia
( 337 ) The Quality of Fried Chips Varies with Cassava (Manihot Esculenta Crantz) Varieties and Soaking Regime
( 338 ) Diversity and Growth Characteristics of Tree Species in the Botanical Gardens, University of Ibadan, Nigeria
( 341 ) Physicochemical and Rheological Characterization of Goat Whey
( 342 ) Performance of Growing Grasscutters Fed Diets with Varying Levels of Soybean Meal Replaced by Brewers Dried Grain
( 344 ) Isolation and Screening of Phosphate Solubilizing Bacteria from Rhizosphere of Tea (Camellia Sinensis L.) on Andisols
( 345 ) Problems Confronting Shea Butter Industry in Nigeria
( 346 ) Utilization of Fish and Mango Wastes on Biological Silage Production
( 349 ) Assessment of Potassium Leaching Behaviour in Selected Soils of Southeastern Nigeria
( 350 ) The Simulation of Egg Hatching Trend of Aedes Aegypti Associated with Rainfall Distribution
( 351 ) Effect of Substrates on Nutritional Composition and Functional Properties of Pleurotus Ostreatus
( 352 ) Duration of Freezing Influences Sensory Attributes of Cassava (Manihot esculenta Crantz) and Plantain (Musa paradisiaca AAB)
( 353 ) Rural Farmers’ Coping Strategies to Effects of Climate Change on Watermelon Production in Igboora, Oyo State, Nigeria
( 355 ) Effect of Body Weight on Reproductive Fitness in Spodoptera Litura (Lepidoptera: Noctuidae)
( 357 ) Investigating Constraints to Poultry Management Practices among Smallholder Farmers in Benue State, Nigeria
( 358 ) Climate Change and Adaptation Strategies: Farmers Perception in Oyo State, Nigeria
( 359 ) The Value of Selected Ecosystem Services: A Case Study of East Mau Forest Ecosystem, Kenya
( 360 ) Farm and Disease Management by Commercial Poultry Farmers
( 361 ) Climate Variability, Incidences and Mitigation Strategies among Poultry Farmers in Abia State, Nigeria
( 362 ) Effects of Different Storage Conditions on Rooting and Shooting Performance of Grapevine (Vitis Vinifera L.) Cuttings in Hydroponic Culture System
( 363 ) Edible Films and Coatings: A Good Idea From Past to Future Technology
( 364 ) The Potential Effect of Zinc Deficiency on Reproductive Profile of Male Rat and its Possible Consequences
( 365 ) Pulsing Preservatives to Prolong Vase Life of Cut Rose Flowers in Bahir Dar, Northwestern Ethiopia
( 366 ) Study of Factors Linked to the Variation in Rentability in the Farming of Broiler Chickens in Northeast Algeria
( 367 ) Report of an Outbreak of Peste Des Petits Ruminants in Iran, Clinical, Epidemiological and Pathological Study
( 368 ) Social, Economical and Environmental Issues of Floriculture Sector Development in Ethiopia