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Molecular Studies on E.Coli Isolate from Milk of Mastatic Cattle with Special Reference to Associated Biochemical Changes in Kaliobea Governorate

Mervat E.I. Radwan

,

Khiary Farouk Abo-Zaid

Mervat E.I. Radwan 1

Khiary Farouk Abo-Zaid 2

  1. Infectious Diseases Department with Veterinary Hospital Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, Egypt 1

  2. First Rescearcher in Serological Unit and Bacteriological Strains Bank of Enterobacteiacae Department, Animal Health Research Institute (AHRI), Giza, Egypt 2

Pages: 1-8

DOI: 10.18488/journal.110.2017.31.1.8

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Article History:

Received: 04 May, 2017
Revised: 07 June, 2017
Accepted: 30 June, 2017
Published: 13 July, 2017


Abstract:

This investigation was performed in Teaching hospital and farm of Benha university in Moshtohor the number of cows in this farm 80 dairy cows that 40 of them had clinical signs of mastitis (inflammation in teats, pain in milking and milk decrease in amount and quality) .When examine these cows to identify the disease which cause these signs . California Mastitis Test (CMT) was performed to determine positive milk samples in the Mastitic targeted   cows. 20 samples of early lactation stage cows out of 40 samples recovered from CMT- positive milk samples. Biochemical and PCR tests were performed to isolates E. Coli from positive milk samples (CMT)  and determined three virulance genes,  eae gene ,SXT1 and SXT2 . The significance of Escherichia coli-induced mastitis and biochemical changes associated to it in cows, due to the presence of virulence genes and wide range resistance to 20 antimicrobials, is concluded.  E.coli cause biochemical changes in mastatic cow as (liver enzymes AST,GPT,TP, ant. oxidative enzymes as CAT, SOD,GST, LD and kidney function as urea and creatinine .E.coli has effect on inflammatory response  in immunity system of mastitic  cow by increase IL6,TNF and CRP.

Contribution/ Originality
This study uses new estimation methodology to virulent toxins of E.Coli by PCR and effects on biochemical changes.

Keywords:

Mastitis serotyping characterization, PCR and biochemical alteration

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Funding:

This study received no specific financial support.

Competing Interests:

The authors declare that they have no competing interests.

Acknowledgement:

Both authors contributed equally to the conception and design of the study.

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