Journal of Cells

Published by: Asian Medical Journals
Online ISSN: 2414-2476
Print ISSN: 2518-2560
Total Citations: 4
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No. 1

Gender as Determinant of the Effect of Yeast Selenium on CD4 T Cells Count Among HIV 1 Positive Children

Pages: 6-14
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Gender as Determinant of the Effect of Yeast Selenium on CD4 T Cells Count Among HIV 1 Positive Children

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DOI: 10.18488/journal.97.2017.21.6.14

Samwel Boaz Otieno

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Samwel Boaz Otieno (2017). Gender as Determinant of the Effect of Yeast Selenium on CD4 T Cells Count Among HIV 1 Positive Children. Journal of Cells, 2(1): 6-14. DOI: 10.18488/journal.97.2017.21.6.14
Background Sex steroid estrogens (E2) have been observed to have synergistic effect on activity of GSH-px on pre-menopausal females taking selenium. GSH-px activity is correlated to CD4 T cell count in HIV positive patients. However no study has been done to determine gender differentiated effect of yeast selenium intake on CD4 T cell count of pre-puberty male and female children. Methods In the study 25 girls and 25 boys  HIV-1 positive on WHO stage three and below were given 50μg yeast selenium and blood samples taken at  zero, three and six months intervals in EDTA vacuutainers. The blood samples were analyzed for CD4 T cell count by ELISA. Results: No significant CD4 T cell count change in the test group = -2.943,p< 0.05 compared to the matched controls t = -1.258 p> 0.05. CD4 T cell count increased among all age groups on test 3-5years (+ 267.1),5-8 years( +200.3)  9-15 years  (+71.2) cells/mm3  and while in matched controls there was a decrease in all age groups 3-5 years(-71),5-8 years (-125) and 9-13years(-10.1)cells/mm3  . No significant difference was observed in CD4 T cell count between boys and Girls {F (df 5, 81) = 1.379 p=0.241} between girls {F ( df 2,32) = 1.531, p= 0.232} and between boys { F ( df 2, 49) = 1.040, p= 0.361} on selenium and between boys and girls {F (df 5, 86) = 1.168, p= 0.332} on control at six months. Conclusion It can be concluded that there was significant increase in CD4 T cell count among the children on selenium, but no significant difference in the effect of selenium on CD4 T cell count between pre-menopausal females and male.

Contribution/ Originality
This study contributes to understanding of the effects of selenium administration to HIV positive children. The yeast selenium provided is commonly available in chemists as nutritional supplements. From this study it is observed that selenium supplementation leads to increase in weight gain and equally increase in CD4 cell count (p< 0.05) between baseline and six month sampling, and delayed progression of the AIDS patients as seen by improvement in WHO clinical staging, and the WAZ-Score.  

The Effect of Phacoemulsification Surgery on Corneal Endothelium

Pages: 1-5
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The Effect of Phacoemulsification Surgery on Corneal Endothelium

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DOI: 10.18488/journal.97.2017.21.1.5

Khalid Alarfaj MD , Abdullah Al Osaily , Moustafa Magliyah

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Khalid Alarfaj MD , Abdullah Al Osaily , Moustafa Magliyah (2017). The Effect of Phacoemulsification Surgery on Corneal Endothelium. Journal of Cells, 2(1): 1-5. DOI: 10.18488/journal.97.2017.21.1.5
Introduction: Around half of world blindness and fifth of visual impairment are caused by cataract. Treatment of cataract and  refractive errors correction improve 73.6% of blindness. Preservation of the Anterior chamber and corneal endothelium are very important aims during cataract surgery. Methodology: This prospective, observational study was done at King Fahd Hospital of the University (KFUH), Alkhobar, Saudi Arabia. After getting the Institutional Review Board (IRB) approval, the study was initiated at KFUH between April 2016 and August 2016. After getting the patients' informed consents, a thorough assessment was done to each patient prior to surgery. All eyes enrolled in the study have undergone phacoemulsification surgery procedure. For each patient, one month after the phacoemulsification surgery , specular microscopy was performed. The phacodynamic parameters and surgery time were compared with the degree of endothelial cells loss, hexagonality and central corneal thickness. Results: The phacodynamic parameters used during phacoemulsification, namely, power (AVG %), ultrasound time, and elliptical motion showed a strong correlation with the change of endothelial cell density (all P< .001). The length of surgery also showed a positive correlation with the amount of endothelial cell loss ( P < .001). All of these parameters did not significantly affect corneal endothelial hexagonality except elliptical motion. Change in central corneal thickness was very minimal. Conclusion: Endothelial loss was significantly associated with phacoemulsification power, ultrasound time, elliptical motion and length of surgery.

Contribution/ Originality
This study is one of very few studies which have investigated the effect of phacoemulsification cataract surgery on density and shape of corneal endothelial cells.