L. F. Reichardt, "Neurotrophin-regulated signalling pathways," Philosophical Transactions of the Royal Society of London, vol. 361, pp. 1545 -1564, 2006.
C. Li, L. Zheng, C. Wang, and X. Zhou, "Absence of nerve growth factor and comparison of tyrosine kinase receptor a levels in mature spermatozoa from oligoasthenozoospermic, asthenozoospermic and fertile men," Clinica Chimica Acta., vol. 411, pp. 1482-1486, 2010.
A. C. Server and E. M. Shooter, "Nerve growth factor," Advances in Protein Chemistry, vol. 31, pp. 339–409, 1977.
G. P. Harper, W. Robert, Glanvillel, and H. Thoenen, "The purification of nerve growth factor from Bovine seminal plasma," Journal of Biological Chemistry, vol. 257, pp. 8541-8548, 1982.
M. B. Levanti, A. Germanà, F. De Carlos, E. Ciriaco, J. A. Vega, and Germana, "Effects of increased nerve growth factor plasma levels on the expression of trkA and p75 in rat testicles," Journal of Anatomy, vol. 208, pp. 373-379, 2006
P. Lonnerberg, O. So Der, M. Parvinen, E. M. Ritze´n, and H. Persson, "B-nerve growth factor influences the expression of androgen-binding protein messenger ribonucleic acid in the rat testis," Biology of Reproduction, vol. 47, pp. 381–388, 1992.
W. Jin, A. Tanaka, G. Watanabe, H. Matsuda, and K. Taya, "Effect of NGF on the motility and acrosome reaction of golden hamster spermatozoa in vitro," Journal of Reproduction and Development, vol. 56, pp. 437-443, 2010.
S. Saeednia, H. Bahadoran, F. Amidi, M. H. Asadi, M. Naji, P. Fallahi, and N. A. Nejad, "Nerve growth factor in human semen: Effect of nerve growth factor on the normozoospermic men during cryopreservation process," Iranian Journal of Basic Medical Sciences, vol. 18, pp. 292–299, 2015.
R. Abir, B. Fisch, S. Jin, M. Barnnet, A. Ben-Haroush, C. Felz, G. Kessler-Icekson, D. Feldberg, S. Nitke, and A. Ao, "Presence of NGF and its receptors in ovaries from human fetuses and adults," Molecular Human Reproduction, vol. 11, pp. 229-236, 2005.
L. L. Robinson, J. Townsend, and R. A. Anderson, "The human fetal testis is a site of expression of neurotrophins and their receptors: Regulation of the germ cell and peritubular cell population," Journal of Clinical Endocrinology and Metabolism, vol. 88, pp. 3943–3951, 2003.
D. Muller, M. S. Davidoff, O. Bargheer, H. J. Paust, W. Pusch, Y. Koeva, D. Jezek, A. F. Holstein, and R. Middendorff, "The expression of neurotrophins and their receptors in the prenatal and adult human testis: Evidence for functions in leydig cells," Histochemistry and Cell Biology, vol. 126, pp. 199–211, 2006.
C. Li, Y. F. Sun, K. L. Yi, Y. H. Ma, Y. L. Sun, W. Zhang, and X. Zhou, "Detection of nerve growth factor (NGF) and its specific receptor (TrkA) in ejaculated bovine sperm, and the effects of NGF on sperm function," Theriogenology, vol. 74, pp. 1615–1622, 2010.
E. Blom, "The evaluation of Bull Semen, with special reference to its use in artificial insemination in Danish," Copenhagen: A/S Carl Fr Mortensen. Abstract in Anim Breed Abstr, vol. 19, p. 648, 1950.
Y. Kitiyanant, B. Chaisalee, and K. Pavasuthipaisit, "Evaluation of the acrosome reaction and viability in buffalo sperm using two staining methods: The effects of heparin and calcium ionophore A23187," Inernational Journal of Andrology, vol. 25, pp. 215-222, 2002.
S. Ramu and R. S. Jeyendran, "The hypo-osmotic swelling test for evaluation of sperm membrane integrity," Methods in Molecular Biology, vol. 927, pp. 21-25, 2013.
K. Arvind, "Application of sperm mediated gene transfer technique for evaluation of invitro fertilization in buffalo (Bubalus Bubalis)," MVSc. Thesis submitted to National Dairy Research Institte, Karnal, Haryana, INDIA, 2012.
P. Bosch, W. E. Roudebush, R. A. McGraw, J. M. DeJarnette, C. E. Marshall, J. Massey, B. Brackett, and G. Benjamin, "Bull spermatozoa express receptors for platelet activating factor," Revista Cientifica, vol. 19, pp. 513-521, 2009.
K. Kawamura, N. Kawamura, S. M. Mulders, M. D. Sollewijn, Gelpke, and A. J. Hsueh, "Ovarian brain-derived neurotrophic factor (BDNF) promotes the development of oocytes into preimplantation embryos," in Proceedings National Academy of Science USA, 2005, pp. 9206–9211.
C. Li, G. Watanabe, Q. Weng, W. Jin, C. Furuta, A. K. Suzuki, M. Kawaguchi, and K. Taya, "Expression of nerve growth factor (NGF), and its receptors trkA and p75 in the reproductive organs of the adult male rats," Zoological Science, vol. 22, pp. 933–937, 2005.
C. Li and Z. Xu, "The potential roles of neurotrophins in male reproduction-a review," Reproduction, vol. 145, pp. 89–95, 2013.
C. Shi, K. Lin, X. Xu, S. Zhang, N. Wang, and M. Fan, "Evidence for the involvement of NGF in human sperm motility," Journal of Biomedical Science and Engineering, vol. 5, pp. 534-541, 2012.
M. H. Perrard, M. Vigier, A. Damestoy, C. Chapat, D. Silandre, B. B. Rudkin, and P. Durand, "Beta-nerve growth factor participates in an auto/paracrine pathway of regulation of the meiotic differentiation of rat spermatocytes," Journal of Cellular Physiology, vol. 210, pp. 51-62, 2007.
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Namagiri Lakshmi S. , Anand Laxmi N. (2015). In Vitro Differential Effect of Nerve Growth Factor on Functional Parameters of Murrah Buffalo Spermatozoa in Low and High Fertile Groups. Animal Review, 2(4): 87-98. DOI: 10.18488/journal.ar/2015.2.4/220.127.116.11
Aim of the present study was to examine the influence of in vitro supplementation of Nerve growth factor (NGF) on functional parameters of Murrah buffalo (Bubalusbubalis) spermatozoa from fresh semen, like, motility, plasmalemma integrity, acrosomal integrity, ATP concentration. Fresh semen samples (n=6) were washed in Tris buffer and divided into two equal parts (control and NGF groups). Only in the NGF group, NGF was added to a final concentration of 50 and 100 ng/ml. The samples were incubated at 37o C for different time intervals in TCM 199 medium supplemented with BSA and the effects were observed at 0, 30, 60 and 120 min of incubation. The experiment was performed in low (LF) and high fertile(HF) groups based on previous three years conception rate and taking cutoff value , below and above this value were categorized as LF and HF group. The mean concentration of the buffalo seminal plasma (n=12) NGF was 67.7±3.25ng/ml and 65.5± 2.76 in LF and HF groups respectively. The concentration of NGF in blood plasma was 83.5±7.82 and 68.6±3.82 in HF and LF groups respectively. The concentration of blood plasma NGF being higher (P<0.05) in HF group. With either dose of NGF in vitro significant effect on the total motility (P<0.05), progressive forward motility (P<0.05) was observed. It could be maintained in HF group till 120 min but in LF group it was restricted to 60 min when compared with their respective control. The functional membrane integrity did not differ significantly between groups (control and NGF treated) in both LF and HF groups with either concentration of NGF. The plasma lemma integrity was significantly less (P<0.05) at 120 min of incubation when compared with the initial value at 0 min of incubation. The percentage of acrosomal intact spermatozoa decreased continuously over a period of time in both the groups. As compared to 0 min of incubation, the significant (P<0.05) loss of acrosome was observed at 60 and 120 min of incubation in LF and HF control groups and NGF supplementation could maintain acrosome integrity in HF group for 60 min where as in LF group it could be maintained significantly only till 30 min of incubation when compared with the initial values of respective groups. Viability of spermatozoa was not significantly different when compared between groups with their respective control, However when compared at different time intervals, the viability of sperms in HF and LF supplemented groups was significantly different from initial values only at 120 min of incubation whereas in respective control groups the loss in viability was significant from 60 min itself. With respect to ATP concentration of spermatozoa in different groups it was observed that 100ng dose could increase the concentration of ATP in HF group significantly (P<0.05) at 60 min of incubation only with NGF in vitro when compared with its respective control. In conclusion, all the parameters decreased significantly at 120 min of incubation when compared with their respective initial values for all the groups. In HF group supplementation of NGF@50ng/ml could maintain functional parameters of spermatozoa for a greater duration of time when compared with LF or control group.
Effects of Dried Rumen Contents Level in Rations on the Performance of Shugor Desert Sheep in Halfa Elgadeda, Kassala State, Sudan
A. B. Osman and M. E. Elimam, "The effects of different levels of mesquite (Prosopis Chilensis) pods on the performance of shugor lambs in Halfa Elgadida, Kassala State, Sudan," Gezira Journal of Agricultural Sciences, vol. 10, pp. 95-101, 2012.
M. Yagoub, "Environmental consideration for sustainable development in greater Darfur State," MSc. Thesis, Institute of Environmental Studies, University of Khartoum, Khartoum, Sudan, 1998.
O. Abusuwar and A. Darrag, Pan Arab integration in forage production and processing. Case study. Khartoum, Sudan: Sudan. Arab Organization for Agricultural Development (AOAD), 2002.
F. M. Elhag, "Effect of chopping and wilting on tropical grass land silage quality in South Kordofan, Sudan," African Livestock Research, vol. 2, pp. 11-14, 1992.
A. M. A. Elhag, "Effects of rangeland protection and forage growth stage on vegetation attributes and sheep and goat selection at North Kordofan, Sudan," Sudan Journal of Agricultural Research, vol. 17, pp. 29-38, 2011.
H. Mohmed, "Evaluation of some range plants for goats in Rahad area, Butana plain, Sudan," M.Sc. Thesis, Faculty of Agricultural Sciences, University of Gezira, Wad Medani, Sudan, 2001.
H. M. Asma, "Utilization of upgrading straws of Sorghum, pearl millet and sesame by Nubian goat in the Sudan," Ph.D. Thesis, Department of Animal Science, Faculty of Agricultural Sciences, University of Gezira, Wad Medani, Sudan, 2007.
M. J. Prokop, J. J. Klopfenstein, and T. Messersmith, "Blood and paunch meal in ruminant rations," Journal of Animal Science, vol. 39, pp. 250-250, 1974.
F. A. El-Yassin, J. P. Fontenot, and H. ChesterJones, "Fermentation characteristics and nutritional value of rumenal contents and blood ensiled with untreated or sodium hydroxide- treated wheat straw," Journal of Animal Science, vol. 69, pp. 1751-1759, 1991.
P. J. Van Soest, Nutritional ecology of the ruminant. Ithaca and London: Comstock Publishing Associates, A Division of Cornell University Press, 1982.
MAW, "Ministry of animal Wealth, Sudan," Anuual Report, 2006.
Feedipedia, "Rumen contents, cattle, fresh." Animal Feed Resources Information System- INRA, CIRAD, AFZ and FAO. Available from www.Feedipedia.org, 2012.
F. G. Rios Rincon, R. M. Bermudez-Hurtado, A. Estrada-Angulo, A. S. Juarez-Reyes, and C. Pujol-Manriquez, "Dried ruminal contents as a substitute for alfalfa hay in growing-finishing diets for feedlot cattle," Journal of Animal and Veterinary Advances, vol. 9, pp. 1526-1530, 2010.
G. W. Snedecor and W. G. Cochran, Statistical methods. Ames, Iowa, USA: Iowa State University Press, 1980.
A. S. M. Eltayeb, "Performance and carcass characteristics of desert sheep fed rabaa (Trianthema Pentandra L) ash alkali treated sorghum stover and concentrates," M. Sc Thesis, Faculty of Agricultural Sciences, University of Gezira, Wad Medani, Sudan, 2010.
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Amani A. B. Osman , Asma H.M. Hamed , Mohmed E. Elimam (2015). Effects of Dried Rumen Contents Level in Rations on the Performance of Shugor Desert Sheep in Halfa Elgadeda, Kassala State, Sudan. Animal Review, 2(4): 81-86. DOI: 10.18488/journal.ar/2015.2.4/18.104.22.168
Twelve Shugor Desert lambs at 9-12 month old and weighing about 25kg were used to study effects of different levels of dried rumen contents in rations on lamb’s performance in Halfa Elgadeda, Kassala State, Sudan. The animals were divided into three groups according to body weight and allocated at random to isonitrogenous and isocaloric rations with 0, 5 or 10% dried rumen contents. They were offered the rations ad lib. in one meal at 8 am. They were allowed a 15 days preliminary period and fattened for 49 days. Barseem (Medicago sativa) was fed once weekly. The data was statistically analyzed according to the completely randomized design. Final BW, daily feed intake, total weight gain and daily weight gain varied among different levels of dried rumen contents in rations, but not significantly (P>0.05). They were highest in animals fed 10% dried rumen contents and least in animals fed no dried rumen contents. Final BW (kg) was 30.27, 31.25 and 31.75 in animals fed 0, 5 and 10% dried rumen contents, respectively. Daily feed intake (kg/day) was 01.20, 01.27 and 01.28, respectively. Total weight gain (kg) was 4.25, 4.63 and 5.75, respectively. Daily weight gain (g) was 150, 165 and 207, respectively. Feed conversion ratio varied among levels of dried rumen contents in rations, but not significantly (P>0.05). It was 8.81, 7.87 and 7.61 in animals fed 0, 5 and 10% dried rumen contents, respectively.
This study is one of very few studies which have investigated the effect of dried rumen content on the performance of shugor lambs. Its primary contribution is finding that dried rumen contents had no negative effects on lambs’ performance and it can be added up to 10% in the rations.