Export to

Contact Us

Abstract of Applied Sciences and Engineering

September 2016, Volume 11, 11, pp 19

Dimerization of Xanthorrhizol Using Peroxidase Enzyme from Broccoli Leaves (Brasicca Oleacea L) and İts İnfluence on the Antioxidant and Antimicrobial Activity

Hanafi ., Candra Irawan, Kartini Afriani, Foliatini ., Joko Untung

Hanafi . 1 

Candra Irawan 1 Kartini Afriani 1 Foliatini . 1 Joko Untung 1 
  1. Polytechnic of AKA Bogor, Bogor, Indonesia 1


Abstract:

Xanthorrhizol, one of phytochemical compound in the extract of Curcuma xanthorrhiza Roxb, is a phenolic type compound which has abundant hydroxyl groups in its structure, thus, it is highly potential to be a hydrogen donor. Several studies showed that the hydrogen donor substances are also have antioxidative and antimicrobial capacity. The hydrogen donor substance from xanthorrhizol can be synthesized with the aid of peroxidase enzyme, via an oxidation mechanism. The as-formed radical product of xanthorrhizol will combine with the other radicals to produce dimer compound of xanthorrhizol. This study was aimed to develop modified xanthorrhizol from Curcuma xanthorrhiza Roxb via dimerization process using peroxidase enzyme from raw extract of broccoli as catalyst. This novel product from modification of xanthorrhizol was expected to have higher antioxidant and antimicrobial activity. This experiment was carried out via several steps, as follows : extraction of xanthorrhizol from curcuma, extraction of peroxidase enzyme from broccoli, dimerization process of xanthorrhizol, characterization of products by UV-Vis spectrophotometry, FTIR spectrophotometry, antioxidant assay using DPPH method and antimicrobial assay. The results showed that the extraction of curcuma using ethyl acetate produce viscose liquid with Rf 0.4 which was predicted as xanthorrhizol. In the further step, this extract was dimerized using peroxidase enzyme extracted from broccoli (protein content of 47.85%, activity of 8.05 unit/mL) and produced a substance which expected to be a new compound since there was a moderate shift in wavelength at UV region. FTIR spectrum showed the decrease in intensity at –OH groups region, the disappeareance of benzene ring at 1514 cm-1, and the significant shift in wavenumber from 1728 cm-1 to 1737 cm-1, which confirmed the breaking of the benzene ring and the formation of ketone group. The antioxidant assay using DPPH method revealed the increase of IC50 from 467.5 ppm to 578,9 ppm (without Tween-80) and 1056.6 ppm (with Tween-80). The result of antimicrobial assay showed that xanthorrhizol and its dimerization product have similar inhibition capacity to the growth of gram positive bacteria of Basillus cereus at concentration of 1% with value of 14 mm (strong) but could not inhibit the growth of gram negative bacteria of E.coli and some fungies (Pestalutiopsis, Penicilium and Aspergillus niger) in the concentration range of 0,2 – 1%.

Keywords:

Xanthorrhizol, Dimerization, Peroxidase enzyme, Antioxidant capacity, Antimicrobial capacity

Statistics:

Google Scholor ideas Microsoft Academic Search bing Google Scholor

Funding:

Competing Interests:

Acknowledgement: